ID   TIAS_IGNAA              Reviewed;         464 AA.
AC   E0SPG5;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   02-NOV-2010, sequence version 1.
DT   25-MAY-2022, entry version 49.
DE   RecName: Full=tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS {ECO:0000255|HAMAP-Rule:MF_01892};
DE            Short=tRNA(Ile2)-agm2C synthetase {ECO:0000255|HAMAP-Rule:MF_01892};
DE            EC=6.3.4.22 {ECO:0000255|HAMAP-Rule:MF_01892};
DE   AltName: Full=tRNA(Ile2) agmatidine synthetase {ECO:0000255|HAMAP-Rule:MF_01892};
GN   Name=tiaS {ECO:0000255|HAMAP-Rule:MF_01892}; OrderedLocusNames=Igag_0055;
OS   Ignisphaera aggregans (strain DSM 17230 / JCM 13409 / AQ1.S1).
OC   Archaea; Crenarchaeota; Thermoprotei; Desulfurococcales;
OC   Desulfurococcaceae; Ignisphaera.
OX   NCBI_TaxID=583356;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 17230 / JCM 13409 / AQ1.S1;
RX   PubMed=21304693; DOI=10.4056/sigs.1072907;
RA   Goker M., Held B., Lapidus A., Nolan M., Spring S., Yasawong M., Lucas S.,
RA   Glavina Del Rio T., Tice H., Cheng J.F., Goodwin L., Tapia R., Pitluck S.,
RA   Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A.,
RA   Palaniappan K., Brambilla E., Land M., Hauser L., Chang Y.J.,
RA   Jeffries C.D., Brettin T., Detter J.C., Han C., Rohde M., Sikorski J.,
RA   Woyke T., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P.,
RA   Kyrpides N.C., Klenk H.P.;
RT   "Complete genome sequence of Ignisphaera aggregans type strain (AQ1.S1).";
RL   Stand. Genomic Sci. 3:66-75(2010).
CC   -!- FUNCTION: ATP-dependent agmatine transferase that catalyzes the
CC       formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34)
CC       of tRNA(Ile2), converting the codon specificity from AUG to AUA.
CC       {ECO:0000255|HAMAP-Rule:MF_01892}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-
CC         agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate;
CC         Xref=Rhea:RHEA:43608, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10626,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58145, ChEBI:CHEBI:82748,
CC         ChEBI:CHEBI:83545, ChEBI:CHEBI:456215; EC=6.3.4.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01892};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01892}.
CC   -!- SIMILARITY: Belongs to the TiaS family. {ECO:0000255|HAMAP-
CC       Rule:MF_01892}.
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DR   EMBL; CP002098; ADM26908.1; -; Genomic_DNA.
DR   AlphaFoldDB; E0SPG5; -.
DR   SMR; E0SPG5; -.
DR   STRING; 583356.Igag_0055; -.
DR   EnsemblBacteria; ADM26908; ADM26908; Igag_0055.
DR   KEGG; iag:Igag_0055; -.
DR   HOGENOM; CLU_675459_0_0_2; -.
DR   OMA; GMCTTYL; -.
DR   Proteomes; UP000001304; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR   GO; GO:0002101; P:tRNA wobble cytosine modification; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01892; tRNA_Ile2_agm2C_synt; 1.
DR   InterPro; IPR013696; DUF1743.
DR   InterPro; IPR024913; tRNA_Ile2__agm2C_synt.
DR   Pfam; PF08489; DUF1743; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome;
KW   tRNA processing.
FT   CHAIN           1..464
FT                   /note="tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS"
FT                   /id="PRO_0000407294"
SQ   SEQUENCE   464 AA;  52750 MW;  7CF064D6DF2DA563 CRC64;
     MYENFCIYHI GLDDFDLLSY GCTTHVATYL LHSLAKRFSS IVFIDYPNLV RLNPSIPWKT
     RGNGAIAIRI AMECTDLNNL LEAAEDIIDE YYEKYGVRTK DVVSSDREPG LVVVRNGIVN
     DMGSLYITAL TDVLLPEIVK KKIDKYGREN IVVSKRYCGR GIVGAAASVG WIAIDSDYTY
     ELLVYRSEKF YSLDRCVNEE SVKYFDSITK DRTFNNIDVD SNRILITSHG RDPILYGVRG
     EDPEVLLKAL DIIRVCEPIS SWTIFRTNQA TDAHAIDRSV SSLRVYRTGK IRVTISSKPS
     IGMGGTVIIN GFDATGSITL AFFRPSYLNR YASMLIPGDV IEANGHVKPW NIGPVFHVEK
     FSVLKLAPLY RCKAPRCPYC RKRLTKMGRG KGYKCDKCGY SVINPDLECE YIERKIRLGL
     YIPPPRALKH LIKPIERYGK EKYRHRYPIE LKLSQITKIL ETSI