ID   TIAS_METV3              Reviewed;         508 AA.
AC   D7DRZ8;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   10-AUG-2010, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS {ECO:0000255|HAMAP-Rule:MF_01892};
DE            Short=tRNA(Ile2)-agm2C synthetase {ECO:0000255|HAMAP-Rule:MF_01892};
DE            EC=6.3.4.22 {ECO:0000255|HAMAP-Rule:MF_01892};
DE   AltName: Full=tRNA(Ile2) agmatidine synthetase {ECO:0000255|HAMAP-Rule:MF_01892};
GN   Name=tiaS {ECO:0000255|HAMAP-Rule:MF_01892}; OrderedLocusNames=Mvol_0248;
OS   Methanococcus voltae (strain ATCC BAA-1334 / A3).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanococcaceae; Methanococcus.
OX   NCBI_TaxID=456320;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-1334 / A3;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L.,
RA   Pitluck S., Lowry S., Clum A., Land M., Hauser L., Kyrpides N.,
RA   Mikhailova N., Whitman W.B., Woyke T.;
RT   "Complete sequence of Methanococcus voltae A3.";
RL   Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ATP-dependent agmatine transferase that catalyzes the
CC       formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34)
CC       of tRNA(Ile2), converting the codon specificity from AUG to AUA.
CC       {ECO:0000255|HAMAP-Rule:MF_01892}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=agmatine + ATP + cytidine(34) in tRNA(Ile2) + H2O = 2-
CC         agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 H(+) + 2 phosphate;
CC         Xref=Rhea:RHEA:43608, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10626,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58145, ChEBI:CHEBI:82748,
CC         ChEBI:CHEBI:83545, ChEBI:CHEBI:456215; EC=6.3.4.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01892};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01892}.
CC   -!- SIMILARITY: Belongs to the TiaS family. {ECO:0000255|HAMAP-
CC       Rule:MF_01892}.
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DR   EMBL; CP002057; ADI35908.1; -; Genomic_DNA.
DR   RefSeq; WP_013179636.1; NC_014222.1.
DR   AlphaFoldDB; D7DRZ8; -.
DR   SMR; D7DRZ8; -.
DR   STRING; 456320.Mvol_0248; -.
DR   EnsemblBacteria; ADI35908; ADI35908; Mvol_0248.
DR   GeneID; 9275468; -.
DR   KEGG; mvo:Mvol_0248; -.
DR   eggNOG; arCOG01115; Archaea.
DR   HOGENOM; CLU_675459_0_0_2; -.
DR   OrthoDB; 58019at2157; -.
DR   Proteomes; UP000007722; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR   GO; GO:0002101; P:tRNA wobble cytosine modification; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01892; tRNA_Ile2_agm2C_synt; 1.
DR   InterPro; IPR013696; DUF1743.
DR   InterPro; IPR024913; tRNA_Ile2__agm2C_synt.
DR   Pfam; PF08489; DUF1743; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding; Reference proteome;
KW   tRNA processing.
FT   CHAIN           1..508
FT                   /note="tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS"
FT                   /id="PRO_0000407295"
FT   DNA_BIND        367..427
FT                   /note="OB"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01892"
SQ   SEQUENCE   508 AA;  57821 MW;  92DEDC60858C1C4C CRC64;
     MYIGIDDTDS REKYCTTYVG TLIVEELLKL GYILEEPRLI RMNPMVKYKT RGNGGVCLKI
     VGKIGAEDTK KNNSNKNNSN NGTPKNIEYD YKQALEDFNR GNIDYEALSK ESNCKSSLKS
     HIKSLKSLKS YRTTLNPEIT EESYKLSKSE YLEVKTIVSS IVEKYTDFDC STTNPGIVLI
     NSRLTRQKKA ILKNYYNSVL TEIVSLDEAE AIIKKVGAEY IKYKKGLGII GSLGAISSYF
     SENQTYTYEL LAYRENDKWG TERYVIDSSV VEMDKMTYPY TFNNVDNNKN IIAPNTKCPV
     LYGIRGVSKE ILFNAKEIVE SENIDKYMIY RTNQGTDHHL RIMNIADARE NTGAILSGYI
     SEEFTEITGG HVLIELTDST GSINCIAYEP TKKFRHIIRE LAIGDLITVY GTIREEPYQL
     NIEKINVVKL NNLYEKVKKC ECGGTLKSKG VSSGYKCNKC GKRLKYDEIP KIQIVRNLRE
     GFYEVPPSAR RHLSMPLSLI NYLPNNLR