ID   TIBC_ECOH1              Reviewed;         406 AA.
AC   Q9S4K6; E3PBM2;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 60.
DE   RecName: Full=Glycosyltransferase TibC;
DE            EC=2.4.-.-;
GN   Name=tibC; OrderedLocusNames=ETEC_2140;
OS   Escherichia coli O78:H11 (strain H10407 / ETEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316401;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=H10407 / ETEC;
RX   PubMed=10417177; DOI=10.1128/iai.67.8.4084-4091.1999;
RA   Lindenthal C., Elsinghorst E.A.;
RT   "Identification of a glycoprotein produced by enterotoxigenic Escherichia
RT   coli.";
RL   Infect. Immun. 67:4084-4091(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H10407 / ETEC;
RX   PubMed=20802035; DOI=10.1128/jb.00710-10;
RA   Crossman L.C., Chaudhuri R.R., Beatson S.A., Wells T.J., Desvaux M.,
RA   Cunningham A.F., Petty N.K., Mahon V., Brinkley C., Hobman J.L.,
RA   Savarino S.J., Turner S.M., Pallen M.J., Penn C.W., Parkhill J.,
RA   Turner A.K., Johnson T.J., Thomson N.R., Smith S.G., Henderson I.R.;
RT   "A commensal gone bad: complete genome sequence of the prototypical
RT   enterotoxigenic Escherichia coli strain H10407.";
RL   J. Bacteriol. 192:5822-5831(2010).
RN   [3]
RP   FUNCTION, AND MUTAGENESIS OF GLU-90; LEU-348 AND CYS-358.
RC   STRAIN=469ST / ETEC, O128:H- / 147/1 / ETEC, O6:H- / 117/86 / ETEC, and
RC   TX-1 / ETEC;
RX   PubMed=11953358; DOI=10.1128/iai.70.5.2264-2270.2002;
RA   Moormann C., Benz I., Schmidt M.A.;
RT   "Functional substitution of the TibC protein of enterotoxigenic Escherichia
RT   coli strains for the autotransporter adhesin heptosyltransferase of the
RT   AIDA system.";
RL   Infect. Immun. 70:2264-2270(2002).
CC   -!- FUNCTION: Glycosylates the TibA adhesin. {ECO:0000269|PubMed:11953358}.
CC   -!- MISCELLANEOUS: Able to functionally replace the autotransporter adhesin
CC       heptosyltransferase (AAH) of the AIDA system in fully restoring
CC       activity to the AIDA-I adhesin depending on the modification of AIDA
CC       with heptose residues.
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DR   EMBL; AF131891; AAD46996.1; -; Genomic_DNA.
DR   EMBL; FN649414; CBJ01642.1; -; Genomic_DNA.
DR   RefSeq; WP_000105682.1; NC_017633.1.
DR   PDB; 4RAP; X-ray; 2.88 A; A/B/C/D/E/F/G/H/I/J/K/L=1-406.
DR   PDBsum; 4RAP; -.
DR   AlphaFoldDB; Q9S4K6; -.
DR   SMR; Q9S4K6; -.
DR   PRIDE; Q9S4K6; -.
DR   EnsemblBacteria; CBJ01642; CBJ01642; ETEC_2140.
DR   KEGG; elh:ETEC_2140; -.
DR   HOGENOM; CLU_044689_0_0_6; -.
DR   OMA; EFYTPWR; -.
DR   Proteomes; UP000006877; Chromosome.
DR   GO; GO:0016757; F:glycosyltransferase activity; IDA:CACAO.
DR   CDD; cd03789; GT9_LPS_heptosyltransferase; 1.
DR   InterPro; IPR030929; Aah/TibC.
DR   InterPro; IPR002201; Glyco_trans_9.
DR   Pfam; PF01075; Glyco_transf_9; 1.
DR   TIGRFAMs; TIGR04414; hepto_Aah_TibC; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Glycosyltransferase; Transferase.
FT   CHAIN           1..406
FT                   /note="Glycosyltransferase TibC"
FT                   /id="PRO_0000076354"
FT   MUTAGEN         90
FT                   /note="E->K: No effect."
FT                   /evidence="ECO:0000269|PubMed:11953358"
FT   MUTAGEN         348
FT                   /note="L->P: No effect."
FT                   /evidence="ECO:0000269|PubMed:11953358"
FT   MUTAGEN         358
FT                   /note="C->R: Loss of glycosyltransferase activity."
FT                   /evidence="ECO:0000269|PubMed:11953358"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           21..23
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          25..28
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          31..36
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          41..47
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   TURN            48..50
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          53..65
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          74..79
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          81..85
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          87..91
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          98..102
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           108..125
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          128..132
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           135..141
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           142..144
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          148..151
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          162..166
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          176..180
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           182..185
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   TURN            187..189
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           190..195
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          218..221
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           228..230
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           237..247
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          251..254
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          259..263
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          266..269
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           283..291
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          294..298
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           303..309
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          314..317
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   STRAND          319..321
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           323..325
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           342..344
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   TURN            359..363
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   TURN            365..368
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           369..372
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   HELIX           376..390
FT                   /evidence="ECO:0007829|PDB:4RAP"
FT   TURN            395..397
FT                   /evidence="ECO:0007829|PDB:4RAP"
SQ   SEQUENCE   406 AA;  46325 MW;  3FDDEA5C9D2FBDD5 CRC64;
     MSTLKNTFFI TPPDTPTQAG PENIFYDFND GARVLLPEGK WHVRLLDADS ENILFCCDVD
     KGWVTSSKKY FVRFRIQVFR QGEETPLLDE TLKLKDRPVL ISFPTGTLGD LLGWFPYAER
     FQSLHKCRLE CTMSQDIIDL LAPQYPQIQF STPDKPRTVA PYATYRVGLY FGGDTNNQPV
     DFRKVGFHRS AGYILGVDPR EAPVRLDLSA PRVIQEPYVC IATQSTCQAK YWNNGTGWSE
     VIAHLKSLGY RVMCIDRDAH YGQGFVWNHI PWGAEDFTGK LPLQERVNLL RHASFFIGLP
     SGLSWLAWAT RIPVVLISGF SLPNSEFYTP WRVFNSHGCY GCWDDTSLNF DHHDFLWCPR
     HKNTDRQFEC TRLITGAQVN GVINKLHRSL TEQGVEATLK KGVSNE