TIC20_PEA
ID TIC20_PEA Reviewed; 253 AA.
AC Q9ZST8;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=Protein TIC 20, chloroplastic;
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 20;
DE Short=PsTIC20;
DE Flags: Precursor;
GN Name=TIC20; Synonyms=IAP21, TIC(21);
OS Pisum sativum (Garden pea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 83-97, FUNCTION,
RP SUBCELLULAR LOCATION, AND INTERACTION WITH TIC22.
RX PubMed=9817756; DOI=10.1083/jcb.143.4.991;
RA Kouranov A., Chen X., Fuks B., Schnell D.J.;
RT "Tic20 and Tic22 are new components of the protein import apparatus at the
RT chloroplast inner envelope membrane.";
RL J. Cell Biol. 143:991-1002(1998).
RN [2]
RP FUNCTION.
RX PubMed=8707818; DOI=10.1083/jcb.134.2.315;
RA Ma Y., Kouranov A., LaSala S.E., Schnell D.J.;
RT "Two components of the chloroplast protein import apparatus, IAP86 and
RT IAP75, interact with the transit sequence during the recognition and
RT translocation of precursor proteins at the outer envelope.";
RL J. Cell Biol. 134:315-327(1996).
RN [3]
RP FUNCTION.
RX PubMed=9412463; DOI=10.1083/jcb.139.7.1677;
RA Kouranov A., Schnell D.J.;
RT "Analysis of the interactions of preproteins with the import machinery over
RT the course of protein import into chloroplasts.";
RL J. Cell Biol. 139:1677-1685(1997).
RN [4]
RP INDUCTION BY HEAT.
RX PubMed=19403728; DOI=10.1104/pp.109.137265;
RA Dutta S., Mohanty S., Tripathy B.C.;
RT "Role of temperature stress on chloroplast biogenesis and protein import in
RT pea.";
RL Plant Physiol. 150:1050-1061(2009).
RN [5]
RP REVIEW.
RX PubMed=20100520; DOI=10.1016/j.bbamcr.2010.01.015;
RA Kovacs-Bogdan E., Soll J., Bolter B.;
RT "Protein import into chloroplasts: the Tic complex and its regulation.";
RL Biochim. Biophys. Acta 1803:740-747(2010).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope. Seems to be specific for
CC photosynthesis-related pre-proteins. {ECO:0000269|PubMed:8707818,
CC ECO:0000269|PubMed:9412463, ECO:0000269|PubMed:9817756}.
CC -!- SUBUNIT: Part of the Tic complex. Interacts with TIC22 and with the Toc
CC complex. {ECO:0000269|PubMed:9817756}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- INDUCTION: Down-regulated by heat. {ECO:0000269|PubMed:19403728}.
CC -!- SIMILARITY: Belongs to the Tic20 family. {ECO:0000305}.
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DR EMBL; AF095285; AAC64607.1; -; mRNA.
DR AlphaFoldDB; Q9ZST8; -.
DR TCDB; 3.A.9.1.1; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR EnsemblPlants; Psat7g252800.1; Psat7g252800.1.cds; Psat7g252800.
DR Gramene; Psat7g252800.1; Psat7g252800.1.cds; Psat7g252800.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR005691; Tic20.
DR PANTHER; PTHR33510; PTHR33510; 1.
DR Pfam; PF16166; TIC20; 1.
DR TIGRFAMs; TIGR00994; 3a0901s05TIC20; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Membrane; Plastid;
KW Plastid inner membrane; Protein transport; Transit peptide; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..82
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:9817756"
FT CHAIN 83..253
FT /note="Protein TIC 20, chloroplastic"
FT /id="PRO_0000413667"
FT TRANSMEM 101..120
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 174..194
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 253 AA; 29157 MW; 3414FCFFBFE04FBA CRC64;
MIQNGGTVSQ GSVLCYACQI PAKVAVSSIR SFWGHSLENK PRGMTFTDMS ATSSLLLSGG
QNFLSRTIPV LPTLHKSSTT PRATKDSSGF RFPPMTKKPR WWWRTLSCIP YLLPFHQAWM
YARTAYHLHP FIPYFQPMTY PFLMAIGTLP RWSLIAYFLI AYLTIVRRKE WPHFFRFHVA
VGMLIEIALQ VTGIVSRWMP RSFYWGKLGM HFWTTAFFVF LFTTIECIRC ALVGMYADVP
FVCDAAYIQI PHE
