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TIC21_ARATH
ID   TIC21_ARATH             Reviewed;         296 AA.
AC   Q9SHU7;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Protein TIC 21, chloroplastic;
DE   AltName: Full=Protein CHLOROPLAST IMPORT APPARATUS 5;
DE            Short=AtCIA5;
DE   AltName: Full=Protein PERMEASE IN CHLOROPLASTS 1;
DE            Short=AtPIC1;
DE   AltName: Full=Translocon at the inner envelope membrane of chloroplasts 21;
DE            Short=AtTIC21;
DE   Flags: Precursor;
GN   Name=TIC21; Synonyms=CIA5, PIC1; OrderedLocusNames=At2g15290;
GN   ORFNames=F27O10.6;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LYS-112 AND GLY-205,
RP   PROTEIN SEQUENCE OF N-TERMINUS, SUBCELLULAR LOCATION, SUBUNIT, AND
RP   DISRUPTION PHENOTYPE.
RX   PubMed=16891400; DOI=10.1105/tpc.106.044305;
RA   Teng Y.S., Su Y.S., Chen L.J., Lee Y.J., Hwang I., Li H.M.;
RT   "Tic21 is an essential translocon component for protein translocation
RT   across the chloroplast inner envelope membrane.";
RL   Plant Cell 18:2247-2257(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=17337631; DOI=10.1105/tpc.106.047407;
RA   Duy D., Wanner G., Meda A.R., von Wiren N., Soll J., Philippar K.;
RT   "PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron
RT   transport.";
RL   Plant Cell 19:986-1006(2007).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=19460081; DOI=10.1111/j.1420-9101.2009.01755.x;
RA   Lv H.X., Guo G.Q., Yang Z.N.;
RT   "Translocons on the inner and outer envelopes of chloroplasts share similar
RT   evolutionary origin in Arabidopsis thaliana.";
RL   J. Evol. Biol. 22:1418-1428(2009).
RN   [7]
RP   FUNCTION, IDENTIFICATION IN TRANSLOCON COMPLEX, AND DISRUPTION PHENOTYPE.
RX   PubMed=19531596; DOI=10.1105/tpc.108.063552;
RA   Kikuchi S., Oishi M., Hirabayashi Y., Lee D.W., Hwang I., Nakai M.;
RT   "A 1-megadalton translocation complex containing Tic20 and Tic21 mediates
RT   chloroplast protein import at the inner envelope membrane.";
RL   Plant Cell 21:1781-1797(2009).
RN   [8]
RP   FUNCTION.
RX   PubMed=21343424; DOI=10.1104/pp.110.170233;
RA   Duy D., Stuebe R., Wanner G., Philippar K.;
RT   "The chloroplast permease PIC1 regulates plant growth and development by
RT   directing homeostasis and transport of iron.";
RL   Plant Physiol. 155:1709-1722(2011).
CC   -!- FUNCTION: Involved in chloroplast protein import across the inner
CC       envelope membrane. Acts also as a chloroplast permease regulating the
CC       iron transport and homeostasis. Involved in the uptake and
CC       sequestration of iron in plastids. {ECO:0000269|PubMed:17337631,
CC       ECO:0000269|PubMed:19460081, ECO:0000269|PubMed:19531596,
CC       ECO:0000269|PubMed:21343424}.
CC   -!- SUBUNIT: Homomultimer. Part of the translocon complex.
CC       {ECO:0000269|PubMed:16891400, ECO:0000269|PubMed:19531596}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC       {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Ubiquitous. Highest expression in green tissues and
CC       very low levels in mature pollen. {ECO:0000269|PubMed:17337631}.
CC   -!- DISRUPTION PHENOTYPE: Seedling lethal when grown on soil. Albino or
CC       chlorotic dwarfed plants that accumulate unprocessed precursor proteins
CC       and chloroplast ferritin clusters when grown in vitro.
CC       {ECO:0000269|PubMed:16891400, ECO:0000269|PubMed:17337631,
CC       ECO:0000269|PubMed:19460081, ECO:0000269|PubMed:19531596}.
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DR   EMBL; AC007267; AAD26904.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC06383.1; -; Genomic_DNA.
DR   EMBL; AY057510; AAL09751.1; -; mRNA.
DR   EMBL; AY065215; AAL38691.1; -; mRNA.
DR   EMBL; AY122931; AAM67464.1; -; mRNA.
DR   PIR; B84527; B84527.
DR   RefSeq; NP_565372.1; NM_127089.4.
DR   AlphaFoldDB; Q9SHU7; -.
DR   STRING; 3702.AT2G15290.1; -.
DR   TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR   PaxDb; Q9SHU7; -.
DR   PRIDE; Q9SHU7; -.
DR   ProteomicsDB; 246465; -.
DR   EnsemblPlants; AT2G15290.1; AT2G15290.1; AT2G15290.
DR   GeneID; 816018; -.
DR   Gramene; AT2G15290.1; AT2G15290.1; AT2G15290.
DR   KEGG; ath:AT2G15290; -.
DR   Araport; AT2G15290; -.
DR   TAIR; locus:2049113; AT2G15290.
DR   eggNOG; ENOG502QTAI; Eukaryota.
DR   HOGENOM; CLU_083138_0_0_1; -.
DR   InParanoid; Q9SHU7; -.
DR   OMA; GIFWAVC; -.
DR   OrthoDB; 1631947at2759; -.
DR   PhylomeDB; Q9SHU7; -.
DR   PRO; PR:Q9SHU7; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SHU7; baseline and differential.
DR   Genevisible; Q9SHU7; AT.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0009941; C:chloroplast envelope; IDA:TAIR.
DR   GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR   GO; GO:0005375; F:copper ion transmembrane transporter activity; IGI:TAIR.
DR   GO; GO:0005381; F:iron ion transmembrane transporter activity; IGI:TAIR.
DR   GO; GO:0042803; F:protein homodimerization activity; IPI:TAIR.
DR   GO; GO:0006875; P:cellular metal ion homeostasis; IMP:TAIR.
DR   GO; GO:0045037; P:protein import into chloroplast stroma; IMP:TAIR.
DR   InterPro; IPR022051; DUF3611.
DR   PANTHER; PTHR34548; PTHR34548; 2.
DR   Pfam; PF12263; DUF3611; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Membrane; Plastid;
KW   Plastid inner membrane; Protein transport; Reference proteome;
KW   Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT   TRANSIT         1..90
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000269|PubMed:16891400"
FT   CHAIN           91..296
FT                   /note="Protein TIC 21, chloroplastic"
FT                   /id="PRO_0000413207"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        156..176
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        208..228
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        250..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         112
FT                   /note="K->C: Strongly reduced protein translocation across
FT                   the inner envelope membrane."
FT                   /evidence="ECO:0000269|PubMed:16891400"
FT   MUTAGEN         205
FT                   /note="G->E: In cia5-1; albino plants."
FT                   /evidence="ECO:0000269|PubMed:16891400"
SQ   SEQUENCE   296 AA;  31276 MW;  7178861D5061E024 CRC64;
     MQSLLLPPAS SSGVSAVALR PGFQHSFNHQ SLSTRSLPLF NPLLLAPKKK TTISSYQSPP
     SLSVYGFQIG GSKPSFTPST VAFSYPTSPS SVPGDNEVDK AKLAQVAKRL EKTSRYFKRL
     GSIGFWGQLV STVVAAVILS FSIVVTGKPT SPATFYATAS GIAAAFVSVF WSFGYIRLSE
     RLRRTSIDPA KAPPRADVVK GLRSGIMVNI LGMGSALLGM QATVGFLVAK ALTTSANPFY
     QGVSQGYSPV LALDVFLVQA SANTLLSHFL GLVCSLELLR SVTVPNSESV VVPKVA
 
 
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