TIC22_PEA
ID TIC22_PEA Reviewed; 252 AA.
AC Q9ZST9;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Protein TIC 22, chloroplastic;
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 22;
DE Short=PsTIC22;
DE Flags: Precursor;
GN Name=TIC22; Synonyms=IAP25;
OS Pisum sativum (Garden pea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-70; 119-127 AND 132-147,
RP FUNCTION, SUBCELLULAR LOCATION, AND INTERACTION WITH TIC20.
RX PubMed=9817756; DOI=10.1083/jcb.143.4.991;
RA Kouranov A., Chen X., Fuks B., Schnell D.J.;
RT "Tic20 and Tic22 are new components of the protein import apparatus at the
RT chloroplast inner envelope membrane.";
RL J. Cell Biol. 143:991-1002(1998).
RN [2]
RP FUNCTION.
RX PubMed=8707818; DOI=10.1083/jcb.134.2.315;
RA Ma Y., Kouranov A., LaSala S.E., Schnell D.J.;
RT "Two components of the chloroplast protein import apparatus, IAP86 and
RT IAP75, interact with the transit sequence during the recognition and
RT translocation of precursor proteins at the outer envelope.";
RL J. Cell Biol. 134:315-327(1996).
RN [3]
RP FUNCTION.
RX PubMed=9412463; DOI=10.1083/jcb.139.7.1677;
RA Kouranov A., Schnell D.J.;
RT "Analysis of the interactions of preproteins with the import machinery over
RT the course of protein import into chloroplasts.";
RL J. Cell Biol. 139:1677-1685(1997).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=10455201; DOI=10.1074/jbc.274.35.25181;
RA Kouranov A., Wang H., Schnell D.J.;
RT "Tic22 is targeted to the intermembrane space of chloroplasts by a novel
RT pathway.";
RL J. Biol. Chem. 274:25181-25186(1999).
RN [5]
RP INTERACTION WITH TIC32.
RX PubMed=15286175; DOI=10.1242/jcs.01265;
RA Nada A., Soll J.;
RT "Inner envelope protein 32 is imported into chloroplasts by a novel
RT pathway.";
RL J. Cell Sci. 117:3975-3982(2004).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=17725642; DOI=10.1111/j.1742-4658.2007.06023.x;
RA Vojta L., Soll J., Boelter B.;
RT "Protein transport in chloroplasts - targeting to the intermembrane
RT space.";
RL FEBS J. 274:5043-5054(2007).
RN [7]
RP INTERACTION WITH TOC64.
RX PubMed=17306301; DOI=10.1016/j.jmb.2007.01.047;
RA Qbadou S., Becker T., Bionda T., Reger K., Ruprecht M., Soll J.,
RA Schleiff E.;
RT "Toc64--a preprotein-receptor at the outer membrane with bipartide
RT function.";
RL J. Mol. Biol. 367:1330-1346(2007).
RN [8]
RP REVIEW.
RX PubMed=20100520; DOI=10.1016/j.bbamcr.2010.01.015;
RA Kovacs-Bogdan E., Soll J., Bolter B.;
RT "Protein import into chloroplasts: the Tic complex and its regulation.";
RL Biochim. Biophys. Acta 1803:740-747(2010).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts.
CC Imported into the intermembrane space via the Toc translocon. May be
CC involved in the import pathway used by proteins without a cleavable N-
CC terminal pre-sequence. {ECO:0000269|PubMed:8707818,
CC ECO:0000269|PubMed:9412463, ECO:0000269|PubMed:9817756}.
CC -!- SUBUNIT: Part of the Tic complex. Interacts with TIC20 and with the Toc
CC complex. Interacts with TIC32 during its import into the chloroplast.
CC {ECO:0000269|PubMed:15286175, ECO:0000269|PubMed:17306301,
CC ECO:0000269|PubMed:9817756}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast intermembrane space
CC {ECO:0000269|PubMed:10455201, ECO:0000269|PubMed:17725642,
CC ECO:0000269|PubMed:9817756}; Peripheral membrane protein
CC {ECO:0000269|PubMed:10455201, ECO:0000269|PubMed:17725642,
CC ECO:0000269|PubMed:9817756}.
CC -!- DOMAIN: The N-terminus (1-54) is necessary and sufficient for targeting
CC to the intermembrane space by an import pathway that is distinct from
CC the general import pathway utilized by stromal preproteins.
CC -!- SIMILARITY: Belongs to the Tic22 family. {ECO:0000305}.
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DR EMBL; AF095284; AAC64606.1; -; mRNA.
DR AlphaFoldDB; Q9ZST9; -.
DR SMR; Q9ZST9; -.
DR IntAct; Q9ZST9; 3.
DR TCDB; 3.A.9.1.1; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR EnsemblPlants; Psat5g042920.1; Psat5g042920.1.cds; Psat5g042920.
DR EnsemblPlants; Psat5g042920.2; Psat5g042920.2.cds; Psat5g042920.
DR EnsemblPlants; Psat5g042920.4; Psat5g042920.4.cds; Psat5g042920.
DR Gramene; Psat5g042920.1; Psat5g042920.1.cds; Psat5g042920.
DR Gramene; Psat5g042920.2; Psat5g042920.2.cds; Psat5g042920.
DR Gramene; Psat5g042920.4; Psat5g042920.4.cds; Psat5g042920.
DR GO; GO:0031972; C:chloroplast intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR005692; Tic22.
DR InterPro; IPR007378; Tic22-like.
DR PANTHER; PTHR33926; PTHR33926; 1.
DR Pfam; PF04278; Tic22; 1.
DR TIGRFAMs; TIGR00995; 3a0901s06TIC22; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Membrane; Plastid;
KW Protein transport; Transit peptide; Transport.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:9817756"
FT CHAIN 51..252
FT /note="Protein TIC 22, chloroplastic"
FT /id="PRO_0000413672"
SQ SEQUENCE 252 AA; 28200 MW; 722F44E1F39C3CD5 CRC64;
MESQGQWNPL LSFSRFINHH SNHLATRLEE TKRLAGTLIQ SHTRTKPAFA ATLTPNHVAK
SLAGTSVYTV SNSDNEFVLM SDAEGAKSIG LLCFRQEDAE AFLAQVRSRK KEFRGGAKVV
PITLDQVYML KVEGIAFRFL PDPVQIKNAL ELRAANRGSF DGVPVFQSDL LVVKKKNKRY
CPVYFSKEDL EYELSKVSRS SKGVGVSQHI MVGSFEDVLK KMELSEKSSG WEDLVFIPPG
KKHSQHMQEV IA
