TIC40_ARATH
ID TIC40_ARATH Reviewed; 447 AA.
AC Q9FMD5;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 130.
DE RecName: Full=Protein TIC 40, chloroplastic;
DE AltName: Full=Protein PIGMENT DEFECTIVE EMBRYO 120;
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 40;
DE Short=AtTIC40;
DE Flags: Precursor;
GN Name=TIC40; Synonyms=PDE120; OrderedLocusNames=At5g16620; ORFNames=MTG13.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TOPOLOGY, AND DISRUPTION PHENOTYPE.
RX PubMed=12805212; DOI=10.1093/emboj/cdg281;
RA Chou M.L., Fitzpatrick L.M., Tu S.L., Budziszewski G., Potter-Lewis S.,
RA Akita M., Levin J.Z., Keegstra K., Li H.M.;
RT "Tic40, a membrane-anchored co-chaperone homolog in the chloroplast protein
RT translocon.";
RL EMBO J. 22:2970-2980(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=15033972; DOI=10.1074/jbc.m401968200;
RA Vojta A., Alavi M., Becker T., Hoermann F., Kuechler M., Soll J.,
RA Thomson R., Schleiff E.;
RT "The protein translocon of the plastid envelopes.";
RL J. Biol. Chem. 279:21401-21405(2004).
RN [6]
RP FUNCTION, AND INTERACTION WITH TIC110.
RX PubMed=15829604; DOI=10.1105/tpc.105.030700;
RA Inaba T., Alvarez-Huerta M., Li M., Bauer J., Ewers C., Kessler F.,
RA Schnell D.J.;
RT "Arabidopsis tic110 is essential for the assembly and function of the
RT protein import machinery of plastids.";
RL Plant Cell 17:1482-1496(2005).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=15659100; DOI=10.1111/j.1365-313x.2004.02307.x;
RA Kovacheva S., Bedard J., Patel R., Dudley P., Twell D., Rios G., Koncz C.,
RA Jarvis P.;
RT "In vivo studies on the roles of Tic110, Tic40 and Hsp93 during chloroplast
RT protein import.";
RL Plant J. 41:412-428(2005).
RN [8]
RP SUBCELLULAR LOCATION, PROTEOLYTIC PROCESSING, AND TOPOLOGY.
RX PubMed=17060496; DOI=10.1083/jcb.200605162;
RA Li M., Schnell D.J.;
RT "Reconstitution of protein targeting to the inner envelope membrane of
RT chloroplasts.";
RL J. Cell Biol. 175:249-259(2006).
RN [9]
RP FUNCTION, INTERACTION WITH TIC110 AND HSP93, AND MUTAGENESIS OF ASN-396;
RP ASN-405 AND ASN-418.
RX PubMed=17158958; DOI=10.1083/jcb.200609172;
RA Chou M.L., Chu C.C., Chen L.J., Akita M., Li H.M.;
RT "Stimulation of transit-peptide release and ATP hydrolysis by a cochaperone
RT during protein import into chloroplasts.";
RL J. Cell Biol. 175:893-900(2006).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH TIC110, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17535810; DOI=10.1074/jbc.m611545200;
RA Bedard J., Kubis S., Bimanadham S., Jarvis P.;
RT "Functional similarity between the chloroplast translocon component, Tic40,
RT and the human co-chaperone, Hsp70-interacting protein (Hip).";
RL J. Biol. Chem. 282:21404-21414(2007).
RN [11]
RP SUBCELLULAR LOCATION, AND PROTEOLYTIC PROCESSING.
RX PubMed=17883373; DOI=10.1111/j.1365-313x.2007.03279.x;
RA Tripp J., Inoue K., Keegstra K., Froehlich J.E.;
RT "A novel serine/proline-rich domain in combination with a transmembrane
RT domain is required for the insertion of AtTic40 into the inner envelope
RT membrane of chloroplasts.";
RL Plant J. 52:824-838(2007).
RN [12]
RP FUNCTION.
RX PubMed=18657235; DOI=10.1111/j.1365-313x.2008.03638.x;
RA Chiu C.C., Li H.M.;
RT "Tic40 is important for reinsertion of proteins from the chloroplast stroma
RT into the inner membrane.";
RL Plant J. 56:793-801(2008).
RN [13]
RP INTERACTION WITH LTD.
RX PubMed=21505433; DOI=10.1038/ncomms1278;
RA Ouyang M., Li X., Ma J., Chi W., Xiao J., Zou M., Chen F., Lu C., Zhang L.;
RT "LTD is a protein required for sorting light-harvesting chlorophyll-binding
RT proteins to the chloroplast SRP pathway.";
RL Nat. Commun. 2:277-277(2011).
RN [14]
RP REVIEW.
RX PubMed=20100520; DOI=10.1016/j.bbamcr.2010.01.015;
RA Kovacs-Bogdan E., Soll J., Bolter B.;
RT "Protein import into chloroplasts: the Tic complex and its regulation.";
RL Biochim. Biophys. Acta 1803:740-747(2010).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. Part
CC of the motor complex consisting of a co-chaperone (TIC40) and a
CC chaperone (HSP93) associated with the import channel (TIC110). Causes
CC the release of bound transit peptides from TIC110 and stimulates ATP
CC hydrolysis by HSP93. Involved in reinsertion of proteins from the
CC chloroplast stroma into the inner membrane.
CC {ECO:0000269|PubMed:12805212, ECO:0000269|PubMed:15659100,
CC ECO:0000269|PubMed:15829604, ECO:0000269|PubMed:17158958,
CC ECO:0000269|PubMed:17535810, ECO:0000269|PubMed:18657235}.
CC -!- SUBUNIT: Part of the Tic complex. Interacts with HSP93, TIC110 and LTD.
CC {ECO:0000269|PubMed:15829604, ECO:0000269|PubMed:17158958,
CC ECO:0000269|PubMed:17535810, ECO:0000269|PubMed:21505433}.
CC -!- INTERACTION:
CC Q9FMD5; Q9FI56: CLPC1; NbExp=3; IntAct=EBI-639157, EBI-2297694;
CC Q9FMD5; Q8LPR9: TIC110; NbExp=3; IntAct=EBI-639157, EBI-639092;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:12805212, ECO:0000269|PubMed:17060496,
CC ECO:0000269|PubMed:17535810, ECO:0000269|PubMed:17883373}; Single-pass
CC membrane protein {ECO:0000269|PubMed:12805212,
CC ECO:0000269|PubMed:17060496, ECO:0000269|PubMed:17535810,
CC ECO:0000269|PubMed:17883373}.
CC -!- TISSUE SPECIFICITY: Expressed in seedlings, flowers, leaves, stems and
CC roots. {ECO:0000269|PubMed:15033972, ECO:0000269|PubMed:15659100}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:15659100}.
CC -!- DOMAIN: The C-terminal half (89-105) of the Ser/Pro-rich region and the
CC transmembrane domain are necessary and sufficient for membrane
CC integration.
CC -!- DOMAIN: The TPR region (238-373) interacts with TIC110.
CC -!- DOMAIN: The STI1 2 domain (386-425) has a stimulatory effect on HSP93
CC ATP hydrolysis.
CC -!- DISRUPTION PHENOTYPE: Small and chlorotic, but not seedling lethal.
CC Defective in chloroplast protein import. {ECO:0000269|PubMed:12805212,
CC ECO:0000269|PubMed:15659100, ECO:0000269|PubMed:17535810}.
CC -!- MISCELLANEOUS: Inserts into the inner envelope membrane from the stroma
CC after import from the cytoplasm. The transit peptide undergoes a two-
CC step processing. The initial cleavage to generate the intermediate
CC found in the stroma is mediated by the stromal processing peptidase
CC (SPP) while the final processing step by a signal peptidase I-type
CC (SPase I), possibly PLSP1, requires association with the inner
CC membrane.
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DR EMBL; AB008270; BAB10189.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92318.1; -; Genomic_DNA.
DR EMBL; AF428299; AAL16131.1; -; mRNA.
DR EMBL; AY093010; AAM13009.1; -; mRNA.
DR EMBL; BT006595; AAP31939.1; -; mRNA.
DR RefSeq; NP_197165.1; NM_121668.4.
DR PDB; 2LNM; NMR; -; A=386-447.
DR PDBsum; 2LNM; -.
DR AlphaFoldDB; Q9FMD5; -.
DR BMRB; Q9FMD5; -.
DR SMR; Q9FMD5; -.
DR BioGRID; 16800; 7.
DR IntAct; Q9FMD5; 3.
DR STRING; 3702.AT5G16620.1; -.
DR TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR iPTMnet; Q9FMD5; -.
DR MetOSite; Q9FMD5; -.
DR PaxDb; Q9FMD5; -.
DR PRIDE; Q9FMD5; -.
DR ProteomicsDB; 234429; -.
DR EnsemblPlants; AT5G16620.1; AT5G16620.1; AT5G16620.
DR GeneID; 831524; -.
DR Gramene; AT5G16620.1; AT5G16620.1; AT5G16620.
DR KEGG; ath:AT5G16620; -.
DR Araport; AT5G16620; -.
DR TAIR; locus:2174155; AT5G16620.
DR eggNOG; KOG1308; Eukaryota.
DR HOGENOM; CLU_038645_1_0_1; -.
DR InParanoid; Q9FMD5; -.
DR OMA; WATSNVK; -.
DR OrthoDB; 950146at2759; -.
DR PhylomeDB; Q9FMD5; -.
DR PRO; PR:Q9FMD5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMD5; baseline and differential.
DR Genevisible; Q9FMD5; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0031897; C:Tic complex; TAS:TAIR.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IMP:TAIR.
DR InterPro; IPR041243; STI1.
DR InterPro; IPR006636; STI1_HS-bd.
DR Pfam; PF17830; STI1; 1.
DR SMART; SM00727; STI1; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Membrane; Plastid; Plastid inner membrane;
KW Protein transport; Reference proteome; Repeat; Transit peptide;
KW Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..43
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 44..76
FT /note="Chloroplast; inner membrane"
FT /evidence="ECO:0000255"
FT CHAIN 77..447
FT /note="Protein TIC 40, chloroplastic"
FT /id="PRO_0000413673"
FT TOPO_DOM 77..104
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..447
FT /note="Stromal"
FT /evidence="ECO:0000255"
FT DOMAIN 310..344
FT /note="STI1 1"
FT DOMAIN 386..425
FT /note="STI1 2"
FT REGION 148..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 231..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 174..200
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..260
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 396
FT /note="N->A: Loss of stimulation of HSP93 ATP hydrolysis."
FT /evidence="ECO:0000269|PubMed:17158958"
FT MUTAGEN 405
FT /note="N->A: Loss of stimulation of HSP93 ATP hydrolysis."
FT /evidence="ECO:0000269|PubMed:17158958"
FT MUTAGEN 418
FT /note="N->A: No effect on stimulation of HSP93 ATP
FT hydrolysis."
FT /evidence="ECO:0000269|PubMed:17158958"
FT HELIX 387..393
FT /evidence="ECO:0007829|PDB:2LNM"
FT HELIX 397..402
FT /evidence="ECO:0007829|PDB:2LNM"
FT HELIX 406..415
FT /evidence="ECO:0007829|PDB:2LNM"
FT HELIX 419..421
FT /evidence="ECO:0007829|PDB:2LNM"
FT HELIX 422..425
FT /evidence="ECO:0007829|PDB:2LNM"
FT HELIX 429..441
FT /evidence="ECO:0007829|PDB:2LNM"
FT STRAND 442..444
FT /evidence="ECO:0007829|PDB:2LNM"
SQ SEQUENCE 447 AA; 48903 MW; 52D3E7222AB461CE CRC64;
MENLTLVSCS ASSPKLLIGC NFTSSLKNPT GFSRRTPNIV LRCSKISASA QSQSPSSRPE
NTGEIVVVKQ RSKAFASIFS SSRDQQTTSV ASPSVPVPPP SSSTIGSPLF WIGVGVGLSA
LFSYVTSNLK KYAMQTAMKT MMNQMNTQNS QFNNSGFPSG SPFPFPFPPQ TSPASSPFQS
QSQSSGATVD VTATKVETPP STKPKPTPAK DIEVDKPSVV LEASKEKKEE KNYAFEDISP
EETTKESPFS NYAEVSETNS PKETRLFEDV LQNGAGPANG ATASEVFQSL GGGKGGPGLS
VEALEKMMED PTVQKMVYPY LPEEMRNPET FKWMLKNPQY RQQLQDMLNN MSGSGEWDKR
MTDTLKNFDL NSPEVKQQFN QIGLTPEEVI SKIMENPDVA MAFQNPRVQA ALMECSENPM
NIMKYQNDKE VMDVFNKISQ LFPGMTG
