TIC56_ARATH
ID TIC56_ARATH Reviewed; 527 AA.
AC Q7Y1W1; Q7Y1Z4; Q9M016;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Protein TIC 56, chloroplastic {ECO:0000303|PubMed:23372012};
DE AltName: Full=Translocon at the inner envelope membrane of chloroplasts 56 {ECO:0000303|PubMed:23372012};
DE Short=AtTIC56 {ECO:0000303|PubMed:23372012};
DE Flags: Precursor;
GN Name=TIC56 {ECO:0000303|PubMed:23372012};
GN OrderedLocusNames=At5g01590 {ECO:0000312|Araport:AT5G01590};
GN ORFNames=F7A7_110 {ECO:0000312|EMBL:CAB82275.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAP41935.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, COMPONENT OF THE 1-MD COMPLEX, SUBUNIT, IDENTIFICATION BY MASS
RP SPECTROMETRY, DEAMIDATION AT ASN-350, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23372012; DOI=10.1126/science.1229262;
RA Kikuchi S., Bedard J., Hirano M., Hirabayashi Y., Oishi M., Imai M.,
RA Takase M., Ide T., Nakai M.;
RT "Uncovering the protein translocon at the chloroplast inner envelope
RT membrane.";
RL Science 339:571-574(2013).
CC -!- FUNCTION: Involved in protein precursor import into chloroplasts. May
CC be part of an intermediate translocation complex acting as a protein-
CC conducting channel at the inner envelope.
CC {ECO:0000269|PubMed:23372012}.
CC -!- SUBUNIT: Part of the Tic complex. Component of the 1-MD complex,
CC composed of TIC20-I, TIC214, TIC100 and TIC56. Interacts with the
CC translocating preproteins. Hydrolysis of ATP is essential for the
CC formation of this complex (PubMed:23372012). The 1-MD complex interacts
CC with TIC21 (By similarity). {ECO:0000250|UniProtKB:Q8GZ79,
CC ECO:0000269|PubMed:23372012}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000269|PubMed:23372012}.
CC -!- DISRUPTION PHENOTYPE: Severe defects during embryogenesis, producing
CC abnormal embryos and thereby leading to a lethality of young seedlings.
CC {ECO:0000269|PubMed:23372012}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82275.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL161946; CAB82275.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90363.1; -; Genomic_DNA.
DR EMBL; AY050767; AAP41935.1; -; mRNA.
DR EMBL; BT008696; AAP40502.1; -; mRNA.
DR PIR; T48180; T48180.
DR RefSeq; NP_568092.4; NM_120237.6.
DR AlphaFoldDB; Q7Y1W1; -.
DR STRING; 3702.AT5G01590.1; -.
DR TCDB; 3.A.9.1.2; the chloroplast envelope protein translocase (cept or tic-toc) family.
DR iPTMnet; Q7Y1W1; -.
DR PaxDb; Q7Y1W1; -.
DR PRIDE; Q7Y1W1; -.
DR ProteomicsDB; 234352; -.
DR EnsemblPlants; AT5G01590.1; AT5G01590.1; AT5G01590.
DR GeneID; 830369; -.
DR Gramene; AT5G01590.1; AT5G01590.1; AT5G01590.
DR KEGG; ath:AT5G01590; -.
DR Araport; AT5G01590; -.
DR TAIR; locus:2149735; AT5G01590.
DR eggNOG; ENOG502QR08; Eukaryota.
DR HOGENOM; CLU_038987_0_0_1; -.
DR InParanoid; Q7Y1W1; -.
DR OMA; EFRESPV; -.
DR OrthoDB; 617126at2759; -.
DR PhylomeDB; Q7Y1W1; -.
DR PRO; PR:Q7Y1W1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q7Y1W1; baseline and differential.
DR Genevisible; Q7Y1W1; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009941; C:chloroplast envelope; HDA:TAIR.
DR GO; GO:0009706; C:chloroplast inner membrane; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0045037; P:protein import into chloroplast stroma; IDA:TAIR.
DR InterPro; IPR025640; GYF_2.
DR InterPro; IPR037471; TIC56.
DR PANTHER; PTHR37755; PTHR37755; 1.
DR Pfam; PF14237; GYF_2; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Protein transport;
KW Reference proteome; Transit peptide; Transport.
FT TRANSIT 1..48
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 49..527
FT /note="Protein TIC 56, chloroplastic"
FT /id="PRO_0000431666"
FT REGION 43..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 491..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..509
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 350
FT /note="Deamidated asparagine"
FT /evidence="ECO:0000269|PubMed:23372012"
FT CONFLICT 259
FT /note="T -> K (in Ref. 3; AAP40502)"
FT /evidence="ECO:0000305"
FT CONFLICT 414
FT /note="M -> V (in Ref. 3; AAP40502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 61625 MW; 2288A8B688D0E79E CRC64;
MSSMNFNPFQ NWFEKPPNPV PSINFVSLAD SFFPKSQSPN FASIGLPKFS KKSPKPETAG
TDEPGPYKQI AEQFLWECEN IPDYRHTPEV DKLLNEDPVF EKKENPSTEE IEAEQKWWES
FRASPVVQFM TRAEEIADDM NKMELEDNDT PYRKEDKDYW RAIPHVPGFD GRPMPRKAIK
SKEESDDKFW DFMKQFLFGL WGFRQRPYPP GRPIDVAQAI GYKRLEKRYY DFIMKTGGWW
YKDRLGRSRG PCEIITLKTA YGAGIIDRDT FIWGEDMDEW APIHMVYGLE PAIATWEVRL
GAAATAFLHK LQKGIPPWVP LKGREPKTYK QLQKEAIESK KRDMAVLEAN GGVWPGVRTP
SHALFLWASG SELTTVLESD HMPNKFIPKQ LRLELAKVIP GLRPWEVISI EQAMDQISYG
GEWYREPLGT YTTGPPYIRE WNRSVMRLFR IFYNLSVRVG QKLERTVPGF DTIMDKVQKD
YDKRIARRMK RREEELREED LKHYSGRTDE DEEEEEEEDD DSNSKKD
