TIF3B_ARATH
ID TIF3B_ARATH Reviewed; 187 AA.
AC Q9C5K8;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Protein TIFY 3B {ECO:0000303|PubMed:17499004};
DE AltName: Full=Jasmonate ZIM domain-containing protein 12 {ECO:0000303|PubMed:17637675, ECO:0000303|PubMed:19151223};
GN Name=TIFY3B {ECO:0000303|PubMed:17499004};
GN Synonyms=JAZ12 {ECO:0000303|PubMed:17637675, ECO:0000303|PubMed:19151223};
GN OrderedLocusNames=At5g20900 {ECO:0000312|Araport:AT5G20900};
GN ORFNames=F22D1.70 {ECO:0000312|EMBL:AF296834};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17637675; DOI=10.1038/nature06006;
RA Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O.,
RA Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L.,
RA Solano R.;
RT "The JAZ family of repressors is the missing link in jasmonate
RT signalling.";
RL Nature 448:666-671(2007).
RN [5]
RP DOMAIN.
RX PubMed=17675405; DOI=10.1105/tpc.107.050708;
RA Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L.,
RA Farmer E.E.;
RT "A downstream mediator in the growth repression limb of the jasmonate
RT pathway.";
RL Plant Cell 19:2470-2483(2007).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
RN [7]
RP INDUCTION BY WOUNDING AND HERBIVORY.
RX PubMed=18223147; DOI=10.1104/pp.107.115691;
RA Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.;
RT "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in
RT response to wounding and herbivory.";
RL Plant Physiol. 146:952-964(2008).
RN [8]
RP FUNCTION, AND INTERACTION WITH TIFY10A/JAZ1; TIFY10B/JAZ2; TIFY11A/JAZ5;
RP TIFY11B/JAZ6; TIFY5A/JAZ8 AND TIFY9/JAZ10.
RX PubMed=19151223; DOI=10.1105/tpc.108.064097;
RA Chung H.S., Howe G.A.;
RT "A critical role for the TIFY motif in repression of jasmonate signaling by
RT a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in
RT Arabidopsis.";
RL Plant Cell 21:131-145(2009).
RN [9]
RP INTERACTION WITH MYC2.
RX PubMed=19309455; DOI=10.1111/j.1365-313x.2009.03852.x;
RA Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.;
RT "The ZIM domain mediates homo- and heteromeric interactions between
RT Arabidopsis JAZ proteins.";
RL Plant J. 59:77-87(2009).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [11]
RP INTERACTION WITH TIFY10A/JAZ1 AND AFPH2/NINJA.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [12]
RP INTERACTION WITH PSEUDOMONAS SYRINGAE HOPZ1A (MICROBIAL INFECTION).
RC STRAIN=cv. Columbia;
RX PubMed=24204266; DOI=10.1371/journal.ppat.1003715;
RA Jiang S., Yao J., Ma K.-W., Zhou H., Song J., He S.Y., Ma W.;
RT "Bacterial effector activates jasmonate signaling by directly targeting JAZ
RT transcriptional repressors.";
RL PLoS Pathog. 9:e1003715-e1003715(2013).
CC -!- FUNCTION: Repressor of jasmonate responses.
CC {ECO:0000269|PubMed:19151223}.
CC -!- SUBUNIT: Interacts with MYC2, AFPH2/NINJA, TIFY10A/JAZ1, TIFY10B/JAZ2,
CC TIFY11A/JAZ5, TIFY11B/JAZ6, TIFY5A/JAZ8 and TIFY9/JAZ10.
CC {ECO:0000269|PubMed:19151223, ECO:0000269|PubMed:19309455,
CC ECO:0000269|PubMed:20360743}.
CC -!- SUBUNIT: (Microbial infection) Interacts with the pathogenic
CC Pseudomonas syringae HopZ1a protein. {ECO:0000269|PubMed:24204266}.
CC -!- INTERACTION:
CC Q9C5K8; Q9SV55: AFPH2; NbExp=7; IntAct=EBI-2312231, EBI-1787005;
CC Q9C5K8; P32068: ASA1; NbExp=3; IntAct=EBI-2312231, EBI-25514634;
CC Q9C5K8; Q9LNJ5: BHLH13; NbExp=7; IntAct=EBI-2312231, EBI-4434261;
CC Q9C5K8; Q39204: MYC2; NbExp=7; IntAct=EBI-2312231, EBI-1792336;
CC Q9C5K8; Q9FIP9: MYC3; NbExp=2; IntAct=EBI-2312231, EBI-15845995;
CC Q9C5K8; O49687: MYC4; NbExp=4; IntAct=EBI-2312231, EBI-15406909;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- INDUCTION: (Microbial infection) Triggered to degradation by the
CC pathogenic Pseudomonas syringae HopZ1a protein in a COI1-dependent
CC manner, thereby activating host jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q9LMA8}.
CC -!- INDUCTION: Up-regulated by wounding and herbivory.
CC {ECO:0000269|PubMed:18223147}.
CC -!- DOMAIN: The jas domain (139-163) is required for interaction with COI1
CC and Pseudomonas syringae HopZ1a. {ECO:0000250|UniProtKB:Q7XPM8,
CC ECO:0000250|UniProtKB:Q9C9E3}.
CC -!- PTM: (Microbial infection) Acetylated by Pseudomonas syringae HopZ1a.
CC {ECO:0000250|UniProtKB:Q9C9E3}.
CC -!- PTM: Ubiquitinated. Targeted for degradation by the SCF(COI1) E3
CC ubiquitin ligase-proteasome pathway during jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q7XPM8}.
CC -!- SIMILARITY: Belongs to the TIFY/JAZ family. {ECO:0000305}.
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DR EMBL; AF296834; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED92902.1; -; Genomic_DNA.
DR EMBL; AF360184; AAK25894.1; -; mRNA.
DR EMBL; AY051013; AAK93690.1; -; mRNA.
DR RefSeq; NP_197590.1; NM_122098.5.
DR AlphaFoldDB; Q9C5K8; -.
DR SMR; Q9C5K8; -.
DR BioGRID; 17489; 23.
DR DIP; DIP-53279N; -.
DR IntAct; Q9C5K8; 16.
DR STRING; 3702.AT5G20900.1; -.
DR iPTMnet; Q9C5K8; -.
DR PaxDb; Q9C5K8; -.
DR PRIDE; Q9C5K8; -.
DR ProteomicsDB; 234354; -.
DR EnsemblPlants; AT5G20900.1; AT5G20900.1; AT5G20900.
DR GeneID; 832214; -.
DR Gramene; AT5G20900.1; AT5G20900.1; AT5G20900.
DR KEGG; ath:AT5G20900; -.
DR Araport; AT5G20900; -.
DR TAIR; locus:2147142; AT5G20900.
DR eggNOG; ENOG502S12Y; Eukaryota.
DR HOGENOM; CLU_088770_2_0_1; -.
DR InParanoid; Q9C5K8; -.
DR OMA; IQPISFC; -.
DR OrthoDB; 1501574at2759; -.
DR PhylomeDB; Q9C5K8; -.
DR PRO; PR:Q9C5K8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9C5K8; baseline and differential.
DR Genevisible; Q9C5K8; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031347; P:regulation of defense response; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009611; P:response to wounding; IBA:GO_Central.
DR InterPro; IPR018467; CCT_CS.
DR InterPro; IPR040390; TIFY/JAZ.
DR InterPro; IPR010399; Tify_dom.
DR PANTHER; PTHR33077; PTHR33077; 1.
DR Pfam; PF09425; Jas_motif; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW Acetylation; Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..187
FT /note="Protein TIFY 3B"
FT /id="PRO_0000300642"
FT DOMAIN 51..86
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 139..163
FT /note="Jas"
FT /evidence="ECO:0000255"
FT MOTIF 141..148
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 36..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 187 AA; 19933 MW; 4F0C66DECBB4F0E1 CRC64;
MTKVKDEPRA SVEGGCGVAD GDGGAAEIGG TGSVEKSINE VRSTEIQTAE PTVPPNQLTI
FFGGSVTVFD GLPSEKVQEI LRIAAKAMET KNSTSISPVS SPALNRAPSF SSTSNVASPA
AQPFPIQPIS FCRSTADLPI ARRHSLQRFL EKRRDRLVNK NPYPTSDFKK TDVPTGNVSI
KEEFPTA
