TIF3_CRYNH
ID TIF3_CRYNH Reviewed; 511 AA.
AC J9VI89;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2013, sequence version 2.
DT 03-AUG-2022, entry version 48.
DE RecName: Full=Probable eukaryotic translation initiation factor 4H {ECO:0000305};
GN Name=TIF3 {ECO:0000303|PubMed:27611567};
GN ORFNames=CNAG_06906 {ECO:0000312|EMBL:AFR94162.2};
OS Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN [1] {ECO:0000312|Proteomes:UP000010091}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA Cuomo C.A., Dietrich F.S.;
RT "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT grubii reveals complex RNA expression and microevolution leading to
RT virulence attenuation.";
RL PLoS Genet. 10:E1004261-E1004261(2014).
RN [2] {ECO:0000305}
RP IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, DISRUPTION
RP PHENOTYPE, AND PHOSPHORYLATION AT SER-334.
RX PubMed=27611567; DOI=10.1371/journal.ppat.1005873;
RA Park H.S., Chow E.W., Fu C., Soderblom E.J., Moseley M.A., Heitman J.,
RA Cardenas M.E.;
RT "Calcineurin Targets Involved in Stress Survival and Fungal Virulence.";
RL PLoS Pathog. 12:e1005873-e1005873(2016).
CC -!- FUNCTION: Probable translation initiation factor. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, P-body {ECO:0000269|PubMed:27611567}.
CC Note=Localizes to P-bodies during response to heat stress.
CC {ECO:0000269|PubMed:27611567}.
CC -!- DISRUPTION PHENOTYPE: Sensitive to dithiothreitol-induced ER stress
CC (PubMed:27611567). Abnormal sexual reproduction; decreases pheromone
CC MFalpha1 expression and abolishes hyphal elongation (PubMed:27611567).
CC {ECO:0000269|PubMed:27611567}.
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DR EMBL; CP003822; AFR94162.2; -; Genomic_DNA.
DR RefSeq; XP_012048004.1; XM_012192614.1.
DR AlphaFoldDB; J9VI89; -.
DR SMR; J9VI89; -.
DR EnsemblFungi; AFR94162; AFR94162; CNAG_06906.
DR GeneID; 23890060; -.
DR VEuPathDB; FungiDB:CNAG_06906; -.
DR HOGENOM; CLU_615667_0_0_1; -.
DR Proteomes; UP000010091; Chromosome 3.
DR GO; GO:0000932; C:P-body; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003743; F:translation initiation factor activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis;
KW RNA-binding.
FT CHAIN 1..511
FT /note="Probable eukaryotic translation initiation factor
FT 4H"
FT /id="PRO_0000451161"
FT DOMAIN 86..162
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 25..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 154..511
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 154..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..320
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 360..414
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..452
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 453..469
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 491..511
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 334
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:27611567"
SQ SEQUENCE 511 AA; 54752 MW; EE5E092758841EBB CRC64;
MAPKKKGQKM ALHEFFEEAA TSGTSWADDD FDLPTAPAAR EESGSGLKRG DPGYFESLPD
RGSRQATFAG APVQREELPL PTVPPFTAFI GNLSFEPDVE DEVRAFFNDL DPVSVRIVKD
PQGKPKGFGY AEFKTQDGLK QALDRSMSQL QGRTIRVNVA EAPSTSRHPP SAAEEASQWR
RSTPLASRES SSQPSRRTGG PSEPAADLDW SVARGAKFTP SAPAAPLSGV RRDSSGPGHT
REPRDPGVSD TADQWRSNKP LAEKVDRDVP PHQAGVAPPI VSPSLADTEQ TWSRGTKLRT
PTTTSRQSSA DSTPSSGAPQ ERRKLNLKPR TAGSPSATAN ATPAAPASGI FGAAKPIDSA
AREKAAEEKL AQREGERRKA REEAEKQKAA AGDKPVEGEK LGWREEKLRS IKAAQDKVAG
KPTTAPATTT NTGAGRKGSA DRAKKDEQGF EQVQPSRKSS QTGATSENKP KKDYSTRPQF
SFAAAAGAIR NDLVEDKDEE EVTKGVEEVK I
