TIF5A_ARATH
ID TIF5A_ARATH Reviewed; 131 AA.
AC Q8LBM2;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Protein TIFY 5A {ECO:0000303|PubMed:17499004};
DE AltName: Full=Jasmonate ZIM domain-containing protein 8 {ECO:0000303|PubMed:17637675};
GN Name=TIFY5A {ECO:0000303|PubMed:17499004};
GN Synonyms=JAZ8 {ECO:0000303|PubMed:17637675};
GN OrderedLocusNames=At1g30135 {ECO:0000312|Araport:AT1G30135};
GN ORFNames=T2H7 {ECO:0000312|EMBL:AC074176};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17637675; DOI=10.1038/nature06006;
RA Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O.,
RA Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L.,
RA Solano R.;
RT "The JAZ family of repressors is the missing link in jasmonate
RT signalling.";
RL Nature 448:666-671(2007).
RN [6]
RP DOMAIN.
RX PubMed=17675405; DOI=10.1105/tpc.107.050708;
RA Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L.,
RA Farmer E.E.;
RT "A downstream mediator in the growth repression limb of the jasmonate
RT pathway.";
RL Plant Cell 19:2470-2483(2007).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
RN [8]
RP INDUCTION BY WOUNDING AND HERBIVORY.
RX PubMed=18223147; DOI=10.1104/pp.107.115691;
RA Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.;
RT "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in
RT response to wounding and herbivory.";
RL Plant Physiol. 146:952-964(2008).
RN [9]
RP FUNCTION, AND INTERACTION WITH TIFY10A/JAZ1; TIFY10B/JAZ2; TIFY6B/JAZ3;
RP TIFY6A/JAZ4; TIFY11A/JAZ5; TIFY11B/JAZ6; TIFY7/JAZ9; TIFY9/JAZ10 AND
RP TIFY3B/JAZ12.
RX PubMed=19151223; DOI=10.1105/tpc.108.064097;
RA Chung H.S., Howe G.A.;
RT "A critical role for the TIFY motif in repression of jasmonate signaling by
RT a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in
RT Arabidopsis.";
RL Plant Cell 21:131-145(2009).
RN [10]
RP INTERACTION WITH MYC2.
RX PubMed=19309455; DOI=10.1111/j.1365-313x.2009.03852.x;
RA Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.;
RT "The ZIM domain mediates homo- and heteromeric interactions between
RT Arabidopsis JAZ proteins.";
RL Plant J. 59:77-87(2009).
RN [11]
RP INTERACTION WITH MYB21 AND MYB24.
RX PubMed=21447791; DOI=10.1105/tpc.111.083089;
RA Song S., Qi T., Huang H., Ren Q., Wu D., Chang C., Peng W., Liu Y.,
RA Peng J., Xie D.;
RT "The Jasmonate-ZIM domain proteins interact with the R2R3-MYB transcription
RT factors MYB21 and MYB24 to affect Jasmonate-regulated stamen development in
RT Arabidopsis.";
RL Plant Cell 23:1000-1013(2011).
RN [12]
RP FUNCTION, DOMAIN, INTERACTION WITH COI1 AND TPL, SUBCELLULAR LOCATION,
RP MUTAGENESIS OF 9-LEU--LEU-13; PHE-14 AND 102-PRO--MET-106, AND INDUCTION BY
RP JASMONATE.
RX PubMed=22327740; DOI=10.1105/tpc.111.093005;
RA Shyu C., Figueroa P., Depew C.L., Cooke T.F., Sheard L.B., Moreno J.E.,
RA Katsir L., Zheng N., Browse J., Howe G.A.;
RT "JAZ8 lacks a canonical degron and has an EAR motif that mediates
RT transcriptional repression of jasmonate responses in Arabidopsis.";
RL Plant Cell 24:536-550(2012).
RN [13]
RP INTERACTION WITH PSEUDOMONAS SYRINGAE HOPZ1A (MICROBIAL INFECTION).
RC STRAIN=cv. Columbia;
RX PubMed=24204266; DOI=10.1371/journal.ppat.1003715;
RA Jiang S., Yao J., Ma K.-W., Zhou H., Song J., He S.Y., Ma W.;
RT "Bacterial effector activates jasmonate signaling by directly targeting JAZ
RT transcriptional repressors.";
RL PLoS Pathog. 9:e1003715-e1003715(2013).
RN [14]
RP FUNCTION, AND INTERACTION WITH RHD6 AND RSL1.
RX PubMed=31988260; DOI=10.1105/tpc.19.00617;
RA Han X., Zhang M., Yang M., Hu Y.;
RT "Arabidopsis JAZ proteins interact with and suppress RHD6 transcription
RT factor to regulate jasmonate-stimulated root hair development.";
RL Plant Cell 32:1049-1062(2020).
CC -!- FUNCTION: Repressor of jasmonate responses. Unable to associate
CC strongly with COI1 in the presence of jasmonoyl-isoleucine (JA-Ile) and
CC is therefore more resistant to JA-mediated-degradation than other
CC TIFY/JAZ proteins. Repress gene expression through direct recruitment
CC of the corepressor TOPLESS to cognate transcription factors
CC (PubMed:19151223, PubMed:22327740). Interacts with and suppresses RHD6
CC and RSL1 transcription factor activities to negatively regulate
CC jasmonate-stimulated root hair development (PubMed:31988260).
CC {ECO:0000269|PubMed:19151223, ECO:0000269|PubMed:22327740,
CC ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: Interacts with TPL and weakly with COI1, but not with
CC AFPH2/NINJA (PubMed:22327740). Interacts with MYC2, MYB21, MYB24,
CC TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY6A/JAZ4, TIFY11A/JAZ5,
CC TIFY11B/JAZ6, TIFY7/JAZ9, TIFY9/JAZ10 and TIFY3B/JAZ12
CC (PubMed:19151223, PubMed:19309455, PubMed:21447791, PubMed:22327740).
CC Interacts with RHD6 and RSL1 (PubMed:31988260).
CC {ECO:0000269|PubMed:19151223, ECO:0000269|PubMed:19309455,
CC ECO:0000269|PubMed:21447791, ECO:0000269|PubMed:22327740,
CC ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: (Microbial infection) Interacts with the pathogenic
CC Pseudomonas syringae HopZ1a protein. {ECO:0000269|PubMed:24204266}.
CC -!- INTERACTION:
CC Q8LBM2; O80748: B-box domain protein 26; NbExp=3; IntAct=EBI-2312143, EBI-15191535;
CC Q8LBM2; Q9LNJ5: BHLH13; NbExp=7; IntAct=EBI-2312143, EBI-4434261;
CC Q8LBM2; Q39204: MYC2; NbExp=7; IntAct=EBI-2312143, EBI-1792336;
CC Q8LBM2; Q9FIP9: MYC3; NbExp=2; IntAct=EBI-2312143, EBI-15845995;
CC Q8LBM2; O49687: MYC4; NbExp=4; IntAct=EBI-2312143, EBI-15406909;
CC Q8LBM2; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-2312143, EBI-4426144;
CC Q8LBM2; Q9LMA8: TIFY10A; NbExp=12; IntAct=EBI-2312143, EBI-1388539;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:22327740}.
CC -!- INDUCTION: (Microbial infection) Triggered to degradation by the
CC pathogenic Pseudomonas syringae HopZ1a protein in a COI1-dependent
CC manner, thereby activating host jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q9LMA8}.
CC -!- INDUCTION: Up-regulated by jasmonate, wounding and herbivory.
CC {ECO:0000269|PubMed:18223147, ECO:0000269|PubMed:22327740}.
CC -!- DOMAIN: The jas domain (105-127) lacks the canonical jasmonoyl-
CC isoleucine (JA-Ile) degron LPIARR that strongly interact with COI1 in
CC the presence of JA-Ile in other TIFY/JAZ proteins (PubMed:22327740).
CC The LxLxL-type EAR (ERF-associated amphiphilic repression) motif (9-13)
CC is required for the interaction with the corepressor TOPLESS
CC (PubMed:22327740). The jas domain (105-127) is required for interaction
CC with Pseudomonas syringae HopZ1a (By similarity).
CC {ECO:0000250|UniProtKB:Q9C9E3, ECO:0000269|PubMed:22327740}.
CC -!- PTM: (Microbial infection) Acetylated by Pseudomonas syringae HopZ1a.
CC {ECO:0000250|UniProtKB:Q9C9E3}.
CC -!- PTM: Ubiquitinated. {ECO:0000305}.
CC -!- MISCELLANEOUS: TIFY5A/JAZ8-mediated repression does not require the
CC tify domain, which, in other TIFY/JAZ proteins, recruits the
CC corepressor TOPLESS through the adapter protein AFPH2/NINJA.
CC {ECO:0000305|PubMed:22327740}.
CC -!- SIMILARITY: Belongs to the TIFY/JAZ family. {ECO:0000305}.
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DR EMBL; AC074176; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE31184.1; -; Genomic_DNA.
DR EMBL; AY087122; AAM64680.1; -; mRNA.
DR EMBL; BT026098; ABG48454.1; -; mRNA.
DR RefSeq; NP_564349.1; NM_102753.4.
DR AlphaFoldDB; Q8LBM2; -.
DR SMR; Q8LBM2; -.
DR BioGRID; 25128; 31.
DR DIP; DIP-53277N; -.
DR IntAct; Q8LBM2; 17.
DR STRING; 3702.AT1G30135.1; -.
DR iPTMnet; Q8LBM2; -.
DR PaxDb; Q8LBM2; -.
DR PRIDE; Q8LBM2; -.
DR EnsemblPlants; AT1G30135.1; AT1G30135.1; AT1G30135.
DR GeneID; 839893; -.
DR Gramene; AT1G30135.1; AT1G30135.1; AT1G30135.
DR KEGG; ath:AT1G30135; -.
DR Araport; AT1G30135; -.
DR TAIR; locus:505006155; AT1G30135.
DR eggNOG; ENOG502S434; Eukaryota.
DR HOGENOM; CLU_113486_0_0_1; -.
DR InParanoid; Q8LBM2; -.
DR OMA; CDITELQ; -.
DR OrthoDB; 1537989at2759; -.
DR PhylomeDB; Q8LBM2; -.
DR PRO; PR:Q8LBM2; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8LBM2; baseline and differential.
DR Genevisible; Q8LBM2; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031347; P:regulation of defense response; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009611; P:response to wounding; IBA:GO_Central.
DR InterPro; IPR018467; CCT_CS.
DR InterPro; IPR040390; TIFY/JAZ.
DR InterPro; IPR010399; Tify_dom.
DR PANTHER; PTHR33077; PTHR33077; 1.
DR Pfam; PF09425; Jas_motif; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW Acetylation; Jasmonic acid signaling pathway; Nucleus; Plant defense;
KW Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..131
FT /note="Protein TIFY 5A"
FT /id="PRO_0000300645"
FT DOMAIN 39..74
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT REGION 14..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 9..13
FT /note="EAR"
FT /evidence="ECO:0000305"
FT MOTIF 105..127
FT /note="Jas"
FT /evidence="ECO:0000255"
FT MOTIF 106..113
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 16..44
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 74..119
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 9..13
FT /note="LELRL->AAAAA: Loss of transcriptional repression
FT activity."
FT /evidence="ECO:0000269|PubMed:22327740"
FT MUTAGEN 9..13
FT /note="Missing: Loss of transcriptional repression
FT activity."
FT /evidence="ECO:0000269|PubMed:22327740"
FT MUTAGEN 9
FT /note="L->A: Decreased transcriptional repression
FT activity."
FT MUTAGEN 10
FT /note="E->A: Decreased transcriptional repression
FT activity."
FT MUTAGEN 11
FT /note="L->A: Loss of transcriptional repression activity."
FT MUTAGEN 12
FT /note="R->A: Decreased transcriptional repression
FT activity."
FT MUTAGEN 13
FT /note="L->A: Decreased transcriptional repression
FT activity."
FT MUTAGEN 14
FT /note="F->A: No effect on transcriptional repression
FT activity."
FT /evidence="ECO:0000269|PubMed:22327740"
FT MUTAGEN 102..106
FT /note="PKASM->LPIAR: Jasmonoyl-isoleucine-dependent
FT interaction with COI1, but no effect on interaction with
FT MYC2."
FT /evidence="ECO:0000269|PubMed:22327740"
SQ SEQUENCE 131 AA; 14770 MW; 4C4F6E24842E5B2B CRC64;
MKLQQNCDLE LRLFPTSYDS DSSDTTSVVE STSSGNPQPN EESQRITIFY NGKMCFSSDV
THLQARSIIS IASREMKTKS SSNGSDPPNK STSFHHNQLP NPKASMKKSL QSFLQKRKIR
IQATSPYHSR R
