TIF6A_ARATH
ID TIF6A_ARATH Reviewed; 310 AA.
AC Q58G47; Q9LP75;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Protein TIFY 6A;
DE AltName: Full=Jasmonate ZIM domain-containing protein 4;
GN Name=TIFY6A; Synonyms=JAZ4; OrderedLocusNames=At1g48500; ORFNames=T1N15.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Redman J.C., Wang W.,
RA Monaghan E.L., Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17637675; DOI=10.1038/nature06006;
RA Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O.,
RA Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L.,
RA Solano R.;
RT "The JAZ family of repressors is the missing link in jasmonate
RT signalling.";
RL Nature 448:666-671(2007).
RN [6]
RP DOMAIN.
RX PubMed=17675405; DOI=10.1105/tpc.107.050708;
RA Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L.,
RA Farmer E.E.;
RT "A downstream mediator in the growth repression limb of the jasmonate
RT pathway.";
RL Plant Cell 19:2470-2483(2007).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
RN [8]
RP INDUCTION BY WOUNDING AND HERBIVORY.
RX PubMed=18223147; DOI=10.1104/pp.107.115691;
RA Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.;
RT "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in
RT response to wounding and herbivory.";
RL Plant Physiol. 146:952-964(2008).
RN [9]
RP FUNCTION, INTERACTION WITH TIFY10A/JAZ1; TIFY6B/JAZ3; TIFY5A/JAZ8;
RP TIFY9/JAZ10 AND TIFY3A/JAZ11, AND SUBUNIT.
RX PubMed=19151223; DOI=10.1105/tpc.108.064097;
RA Chung H.S., Howe G.A.;
RT "A critical role for the TIFY motif in repression of jasmonate signaling by
RT a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in
RT Arabidopsis.";
RL Plant Cell 21:131-145(2009).
RN [10]
RP INTERACTION WITH MYC2, AND SUBUNIT.
RX PubMed=19309455; DOI=10.1111/j.1365-313x.2009.03852.x;
RA Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.;
RT "The ZIM domain mediates homo- and heteromeric interactions between
RT Arabidopsis JAZ proteins.";
RL Plant J. 59:77-87(2009).
RN [11]
RP INTERACTION WITH AFPH2/NINJA.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [12]
RP FUNCTION, AND INTERACTION WITH RHD6 AND RSL1.
RX PubMed=31988260; DOI=10.1105/tpc.19.00617;
RA Han X., Zhang M., Yang M., Hu Y.;
RT "Arabidopsis JAZ proteins interact with and suppress RHD6 transcription
RT factor to regulate jasmonate-stimulated root hair development.";
RL Plant Cell 32:1049-1062(2020).
CC -!- FUNCTION: Repressor of jasmonate responses (PubMed:19151223). Interacts
CC with and suppresses RHD6 and RSL1 transcription factor activities to
CC negatively regulate jasmonate-stimulated root hair development
CC (PubMed:31988260). {ECO:0000269|PubMed:19151223,
CC ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: Homo- and heterodimer. Interacts with MYC2, AFPH2/NINJA,
CC TIFY10A/JAZ1, TIFY6B/JAZ3, TIFY5A/JAZ8, TIFY9/JAZ10 and TIFY3A/JAZ11
CC (PubMed:19151223, PubMed:19309455, PubMed:20360743). Interacts with
CC RHD6 and RSL1 (PubMed:31988260). {ECO:0000269|PubMed:19151223,
CC ECO:0000269|PubMed:19309455, ECO:0000269|PubMed:20360743,
CC ECO:0000269|PubMed:31988260}.
CC -!- INTERACTION:
CC Q58G47; Q9SV55: AFPH2; NbExp=2; IntAct=EBI-2312053, EBI-1787005;
CC Q58G47; Q9LNJ5: BHLH13; NbExp=4; IntAct=EBI-2312053, EBI-4434261;
CC Q58G47; Q9LVI4: TIFY6B; NbExp=3; IntAct=EBI-2312053, EBI-1792431;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q58G47-1; Sequence=Displayed;
CC -!- INDUCTION: Up-regulated by wounding and herbivory.
CC {ECO:0000269|PubMed:18223147}.
CC -!- DOMAIN: The jas domain (259-284) is required for interaction with COI1.
CC {ECO:0000250|UniProtKB:Q7XPM8}.
CC -!- PTM: Ubiquitinated. Targeted for degradation by the SCF(COI1) E3
CC ubiquitin ligase-proteasome pathway during jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q7XPM8}.
CC -!- SIMILARITY: Belongs to the TIFY/JAZ family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF79697.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC020889; AAF79697.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32302.1; -; Genomic_DNA.
DR EMBL; AY954762; AAX55088.1; -; mRNA.
DR EMBL; DQ132675; AAZ52705.1; -; mRNA.
DR PIR; H96524; H96524.
DR RefSeq; NP_175283.2; NM_103746.3. [Q58G47-1]
DR AlphaFoldDB; Q58G47; -.
DR SMR; Q58G47; -.
DR BioGRID; 26496; 21.
DR DIP; DIP-53273N; -.
DR IntAct; Q58G47; 12.
DR STRING; 3702.AT1G48500.1; -.
DR PaxDb; Q58G47; -.
DR PRIDE; Q58G47; -.
DR EnsemblPlants; AT1G48500.1; AT1G48500.1; AT1G48500. [Q58G47-1]
DR GeneID; 841271; -.
DR Gramene; AT1G48500.1; AT1G48500.1; AT1G48500. [Q58G47-1]
DR KEGG; ath:AT1G48500; -.
DR Araport; AT1G48500; -.
DR TAIR; locus:2198018; AT1G48500.
DR eggNOG; ENOG502QWAG; Eukaryota.
DR InParanoid; Q58G47; -.
DR OMA; MSECVSC; -.
DR PhylomeDB; Q58G47; -.
DR PRO; PR:Q58G47; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q58G47; baseline and differential.
DR Genevisible; Q58G47; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009861; P:jasmonic acid and ethylene-dependent systemic resistance; IMP:TAIR.
DR GO; GO:0009867; P:jasmonic acid mediated signaling pathway; IMP:TAIR.
DR GO; GO:0031347; P:regulation of defense response; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009409; P:response to cold; IMP:TAIR.
DR GO; GO:0009611; P:response to wounding; IBA:GO_Central.
DR InterPro; IPR018467; CCT_CS.
DR InterPro; IPR040390; TIFY/JAZ.
DR InterPro; IPR010399; Tify_dom.
DR PANTHER; PTHR33077; PTHR33077; 1.
DR Pfam; PF09425; Jas_motif; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Jasmonic acid signaling pathway; Nucleus;
KW Plant defense; Reference proteome; Transcription; Transcription regulation;
KW Ubl conjugation.
FT CHAIN 1..310
FT /note="Protein TIFY 6A"
FT /id="PRO_0000300647"
FT DOMAIN 141..176
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT MOTIF 259..284
FT /note="Jas"
FT /evidence="ECO:0000255"
FT MOTIF 261..268
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
SQ SEQUENCE 310 AA; 33632 MW; 83E08ACF3E387ADC CRC64;
MERDFLGLGS KLSPITVKEE TNEDSAPSRG MMDWSFSSKV GSGPQFLSFG TSQQETRVNT
VNDHLLSSAA MDQNQRTYFS SLQEDRVFPG SSQQDQTTIT VSMSEPNYIN SFINHQHLGG
SPIMAPPVSV FPAPTTIRSS SKPLPPQLTI FYAGSVLVYQ DIAPEKAQAI MLLAGNGPHA
KPVSQPKPQK LVHHSLPTTD PPTMPPSFLP SISYIVSETR SSGSNGVTGL GPTKTKASLA
STRNNQTAAF SMAPTVGLPQ TRKASLARFL EKRKERVINV SPYYVDNKSS IDCRTLMSEC
VSCPPAHHLH
