TIF7_ARATH
ID TIF7_ARATH Reviewed; 267 AA.
AC Q8W4J8; A8MSB0; F4I6W0; Q0WLN3; Q2V4D4; Q9CAC0;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Protein TIFY 7;
DE AltName: Full=Jasmonate ZIM domain-containing protein 9;
GN Name=TIFY7; Synonyms=JAZ9; OrderedLocusNames=At1g70700; ORFNames=F5A18.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 209-267 (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INDUCTION BY JASMONATE.
RX PubMed=17637677; DOI=10.1038/nature05960;
RA Thines B., Katsir L., Melotto M., Niu Y., Mandaokar A., Liu G., Nomura K.,
RA He S.Y., Howe G.A., Browse J.;
RT "JAZ repressor proteins are targets of the SCF(COI1) complex during
RT jasmonate signalling.";
RL Nature 448:661-665(2007).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17637675; DOI=10.1038/nature06006;
RA Chini A., Fonseca S., Fernandez G., Adie B., Chico J.M., Lorenzo O.,
RA Garcia-Casado G., Lopez-Vidriero I., Lozano F.M., Ponce M.R., Micol J.L.,
RA Solano R.;
RT "The JAZ family of repressors is the missing link in jasmonate
RT signalling.";
RL Nature 448:666-671(2007).
RN [7]
RP DOMAIN.
RX PubMed=17675405; DOI=10.1105/tpc.107.050708;
RA Yan Y., Stolz S., Chetelat A., Reymond P., Pagni M., Dubugnon L.,
RA Farmer E.E.;
RT "A downstream mediator in the growth repression limb of the jasmonate
RT pathway.";
RL Plant Cell 19:2470-2483(2007).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17499004; DOI=10.1016/j.tplants.2007.04.004;
RA Vanholme B., Grunewald W., Bateman A., Kohchi T., Gheysen G.;
RT "The tify family previously known as ZIM.";
RL Trends Plant Sci. 12:239-244(2007).
RN [9]
RP INTERACTION WITH COI1 AND MYC2, DOMAIN, AND MUTAGENESIS OF 223-ARG-LYS-224.
RX PubMed=18547396; DOI=10.1111/j.1365-313x.2008.03566.x;
RA Melotto M., Mecey C., Niu Y., Chung H.S., Katsir L., Yao J., Zeng W.,
RA Thines B., Staswick P., Browse J., Howe G.A., He S.Y.;
RT "A critical role of two positively charged amino acids in the Jas motif of
RT Arabidopsis JAZ proteins in mediating coronatine- and jasmonoyl isoleucine-
RT dependent interactions with the COI1 F-box protein.";
RL Plant J. 55:979-988(2008).
RN [10]
RP INDUCTION BY WOUNDING AND HERBIVORY.
RX PubMed=18223147; DOI=10.1104/pp.107.115691;
RA Chung H.S., Koo A.J., Gao X., Jayanty S., Thines B., Jones A.D., Howe G.A.;
RT "Regulation and function of Arabidopsis JASMONATE ZIM-domain genes in
RT response to wounding and herbivory.";
RL Plant Physiol. 146:952-964(2008).
RN [11]
RP FUNCTION, AND INTERACTION WITH TIFY10A/JAZ1; TIFY10B/JAZ2; TIFY6B/JAZ3;
RP TIFY5A/JAZ8; TIFY9/JAZ10 AND TIFY3A/JAZ11.
RX PubMed=19151223; DOI=10.1105/tpc.108.064097;
RA Chung H.S., Howe G.A.;
RT "A critical role for the TIFY motif in repression of jasmonate signaling by
RT a stabilized splice variant of the JASMONATE ZIM-domain protein JAZ10 in
RT Arabidopsis.";
RL Plant Cell 21:131-145(2009).
RN [12]
RP INTERACTION WITH MYC2, AND SUBUNIT.
RX PubMed=19309455; DOI=10.1111/j.1365-313x.2009.03852.x;
RA Chini A., Fonseca S., Chico J.M., Fernandez-Calvo P., Solano R.;
RT "The ZIM domain mediates homo- and heteromeric interactions between
RT Arabidopsis JAZ proteins.";
RL Plant J. 59:77-87(2009).
RN [13]
RP INTERACTION WITH AFPH2/NINJA.
RX PubMed=20360743; DOI=10.1038/nature08854;
RA Pauwels L., Barbero G.F., Geerinck J., Tilleman S., Grunewald W.,
RA Perez A.C., Chico J.M., Bossche R.V., Sewell J., Gil E., Garcia-Casado G.,
RA Witters E., Inze D., Long J.A., De Jaeger G., Solano R., Goossens A.;
RT "NINJA connects the co-repressor TOPLESS to jasmonate signalling.";
RL Nature 464:788-791(2010).
RN [14]
RP INTERACTION WITH MYC2; MYC3 AND MYC4.
RX PubMed=21321051; DOI=10.1093/jxb/erq408;
RA Niu Y., Figueroa P., Browse J.;
RT "Characterization of JAZ-interacting bHLH transcription factors that
RT regulate jasmonate responses in Arabidopsis.";
RL J. Exp. Bot. 62:2143-2154(2011).
RN [15]
RP INTERACTION WITH MYC2 AND COI1, SUBCELLULAR LOCATION, AND MUTAGENESIS OF
RP 223-ARG-LYS-224 AND 233-LYS--LYS-235.
RX PubMed=23169619; DOI=10.1073/pnas.1210054109;
RA Withers J., Yao J., Mecey C., Howe G.A., Melotto M., He S.Y.;
RT "Transcription factor-dependent nuclear localization of a transcriptional
RT repressor in jasmonate hormone signaling.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:20148-20153(2012).
RN [16]
RP FUNCTION, AND INTERACTION WITH RHD6 AND RSL1.
RX PubMed=31988260; DOI=10.1105/tpc.19.00617;
RA Han X., Zhang M., Yang M., Hu Y.;
RT "Arabidopsis JAZ proteins interact with and suppress RHD6 transcription
RT factor to regulate jasmonate-stimulated root hair development.";
RL Plant Cell 32:1049-1062(2020).
RN [17]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 218-239.
RX PubMed=26258305; DOI=10.1038/nature14661;
RA Zhang F., Yao J., Ke J., Zhang L., Lam V.Q., Xin X.F., Zhou X.E., Chen J.,
RA Brunzelle J., Griffin P.R., Zhou M., Xu H.E., Melcher K., He S.Y.;
RT "Structural basis of JAZ repression of MYC transcription factors in
RT jasmonate signalling.";
RL Nature 525:269-273(2015).
CC -!- FUNCTION: Repressor of jasmonate responses. Jasmonoyl-isoleucine (JA-
CC Ile) specifically promotes COI1-TIFY7/JAZ9 interaction
CC (PubMed:19151223). Interacts with and suppresses RHD6 and RSL1
CC transcription factor activities to negatively regulate jasmonate-
CC stimulated root hair development (PubMed:31988260).
CC {ECO:0000269|PubMed:19151223, ECO:0000269|PubMed:31988260}.
CC -!- SUBUNIT: Homo- and heterodimer. Interacts with MYC2, MYC3, MYC4, COI1,
CC AFPH2/NINJA, TIFY10A/JAZ1, TIFY10B/JAZ2, TIFY6B/JAZ3, TIFY5A/JAZ8,
CC TIFY9/JAZ10 and TIFY3A/JAZ11 (PubMed:18547396, PubMed:19151223,
CC PubMed:19309455, PubMed:20360743, PubMed:21321051, PubMed:23169619).
CC Interacts with RHD6 and RSL1 (PubMed:31988260).
CC {ECO:0000269|PubMed:18547396, ECO:0000269|PubMed:19151223,
CC ECO:0000269|PubMed:19309455, ECO:0000269|PubMed:20360743,
CC ECO:0000269|PubMed:21321051, ECO:0000269|PubMed:23169619,
CC ECO:0000269|PubMed:31988260}.
CC -!- INTERACTION:
CC Q8W4J8; Q9SV55: AFPH2; NbExp=9; IntAct=EBI-1792583, EBI-1787005;
CC Q8W4J8; Q9FIW5: ANAC094; NbExp=3; IntAct=EBI-1792583, EBI-25522986;
CC Q8W4J8; Q9LNJ5: BHLH13; NbExp=5; IntAct=EBI-1792583, EBI-4434261;
CC Q8W4J8; O04197: COI1; NbExp=7; IntAct=EBI-1792583, EBI-401159;
CC Q8W4J8; Q9LQT8: GAI; NbExp=10; IntAct=EBI-1792583, EBI-963606;
CC Q8W4J8; Q39204: MYC2; NbExp=19; IntAct=EBI-1792583, EBI-1792336;
CC Q8W4J8; Q9FIP9: MYC3; NbExp=5; IntAct=EBI-1792583, EBI-15845995;
CC Q8W4J8; O49687: MYC4; NbExp=8; IntAct=EBI-1792583, EBI-15406909;
CC Q8W4J8; Q9SLH3: RGA; NbExp=4; IntAct=EBI-1792583, EBI-963624;
CC Q8W4J8; Q8GXW1: RGL2; NbExp=3; IntAct=EBI-1792583, EBI-963665;
CC Q8W4J8; Q9LF53: RGL3; NbExp=3; IntAct=EBI-1792583, EBI-15681313;
CC Q8W4J8; Q9C9L2: TCP15; NbExp=3; IntAct=EBI-1792583, EBI-4426144;
CC Q8W4J8; Q9MAH8: TCP3; NbExp=3; IntAct=EBI-1792583, EBI-25522447;
CC Q8W4J8; Q9LVI4: TIFY6B; NbExp=4; IntAct=EBI-1792583, EBI-1792431;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00768,
CC ECO:0000269|PubMed:23169619}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8W4J8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8W4J8-2; Sequence=VSP_027851;
CC -!- INDUCTION: Up-regulated by jasmonate, wounding and herbivory.
CC {ECO:0000269|PubMed:17637677, ECO:0000269|PubMed:18223147}.
CC -!- DOMAIN: The jas domain (220-244) is necessary and sufficient for
CC interaction with COI1. {ECO:0000269|PubMed:17675405,
CC ECO:0000269|PubMed:18547396}.
CC -!- PTM: Ubiquitinated. Targeted for degradation by the SCF(COI1) E3
CC ubiquitin ligase-proteasome pathway during jasmonate signaling.
CC {ECO:0000250|UniProtKB:Q7XPM8}.
CC -!- SIMILARITY: Belongs to the TIFY/JAZ family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG52332.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF01974.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC011663; AAG52332.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE35101.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35102.1; -; Genomic_DNA.
DR EMBL; AY062515; AAL32593.1; -; mRNA.
DR EMBL; AY081676; AAM10238.1; -; mRNA.
DR EMBL; AK230165; BAF01974.1; ALT_INIT; mRNA.
DR PIR; D96731; D96731.
DR RefSeq; NP_001031264.4; NM_001036187.5. [Q8W4J8-2]
DR RefSeq; NP_177227.5; NM_105738.8. [Q8W4J8-1]
DR PDB; 4RS9; X-ray; 1.95 A; B=218-239.
DR PDB; 4YWC; X-ray; 2.40 A; C/D=218-239.
DR PDBsum; 4RS9; -.
DR PDBsum; 4YWC; -.
DR AlphaFoldDB; Q8W4J8; -.
DR SMR; Q8W4J8; -.
DR BioGRID; 28627; 29.
DR DIP; DIP-52213N; -.
DR ELM; Q8W4J8; -.
DR IntAct; Q8W4J8; 26.
DR STRING; 3702.AT1G70700.1; -.
DR PaxDb; Q8W4J8; -.
DR PRIDE; Q8W4J8; -.
DR ProteomicsDB; 234355; -. [Q8W4J8-1]
DR EnsemblPlants; AT1G70700.1; AT1G70700.1; AT1G70700. [Q8W4J8-1]
DR EnsemblPlants; AT1G70700.2; AT1G70700.2; AT1G70700. [Q8W4J8-2]
DR GeneID; 843407; -.
DR Gramene; AT1G70700.1; AT1G70700.1; AT1G70700. [Q8W4J8-1]
DR Gramene; AT1G70700.2; AT1G70700.2; AT1G70700. [Q8W4J8-2]
DR KEGG; ath:AT1G70700; -.
DR Araport; AT1G70700; -.
DR TAIR; locus:2033607; AT1G70700.
DR eggNOG; ENOG502RIU4; Eukaryota.
DR HOGENOM; CLU_1257656_0_0_1; -.
DR InParanoid; Q8W4J8; -.
DR OMA; RCRDRPV; -.
DR PRO; PR:Q8W4J8; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8W4J8; baseline and differential.
DR Genevisible; Q8W4J8; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0031347; P:regulation of defense response; IBA:GO_Central.
DR GO; GO:2000022; P:regulation of jasmonic acid mediated signaling pathway; IBA:GO_Central.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR GO; GO:0009611; P:response to wounding; IBA:GO_Central.
DR InterPro; IPR018467; CCT_CS.
DR InterPro; IPR040390; TIFY/JAZ.
DR InterPro; IPR010399; Tify_dom.
DR PANTHER; PTHR33077; PTHR33077; 1.
DR Pfam; PF09425; Jas_motif; 1.
DR Pfam; PF06200; tify; 1.
DR SMART; SM00979; TIFY; 1.
DR PROSITE; PS51320; TIFY; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Jasmonic acid signaling pathway;
KW Nucleus; Plant defense; Reference proteome; Transcription;
KW Transcription regulation; Ubl conjugation.
FT CHAIN 1..267
FT /note="Protein TIFY 7"
FT /id="PRO_0000300649"
FT DOMAIN 113..148
FT /note="Tify"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00650"
FT MOTIF 220..244
FT /note="Jas"
FT /evidence="ECO:0000255"
FT MOTIF 222..229
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT VAR_SEQ 167..190
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_027851"
FT MUTAGEN 223..224
FT /note="RK->AA: Loss of interaction with COI1 and resistant
FT to coronatine-mediated degradation. No effect on MYC2
FT binding and nuclear localization."
FT /evidence="ECO:0000269|PubMed:18547396,
FT ECO:0000269|PubMed:23169619"
FT MUTAGEN 223
FT /note="R->A: No effect."
FT MUTAGEN 224
FT /note="K->A: No effect."
FT MUTAGEN 233..235
FT /note="KRK->AAA: Loss of interaction with MYC2 and COI1 and
FT loss of nuclear localization."
FT /evidence="ECO:0000269|PubMed:23169619"
FT MUTAGEN 233..235
FT /note="KRK->ARA: Sensitive to coronatine-mediated
FT degradation and no effect on nuclear localization."
FT /evidence="ECO:0000269|PubMed:23169619"
FT MUTAGEN 233
FT /note="K->A: No effect on nuclear localization."
FT MUTAGEN 234
FT /note="R->A: Loss of interaction with COI1 and resistant to
FT coronatine-mediated degradation. Loss of nuclear
FT localization."
FT MUTAGEN 235
FT /note="K->A: No effect on interaction with MYC2 and nuclear
FT localization."
FT CONFLICT 133
FT /note="D -> N (in Ref. 3; AAL32593/AAM10238)"
FT /evidence="ECO:0000305"
FT HELIX 219..234
FT /evidence="ECO:0007829|PDB:4RS9"
SQ SEQUENCE 267 AA; 28842 MW; 855ACCF25C47B650 CRC64;
MERDFLGLSD KQYLSNNVKH EVNDDAVEER GLSTKAAREW GKSKVFATSS FMPSSDFQEA
KAFPGAYQWG SVSAANVFRR CQFGGAFQNA TPLLLGGSVP LPTHPSLVPR VASSGSSPQL
TIFYGGTISV FNDISPDKAQ AIMLCAGNGL KGETGDSKPV REAERMYGKQ IHNTAATSSS
SATHTDNFSR CRDTPVAATN AMSMIESFNA APRNMIPSVP QARKASLARF LEKRKERLMS
AMPYKKMLLD LSTGESSGMN YSSTSPT
