ID   TIFA_XENLA              Reviewed;         178 AA.
AC   Q2MHQ9;
DT   09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2006, sequence version 1.
DT   03-AUG-2022, entry version 51.
DE   RecName: Full=TRAF-interacting protein with FHA domain-containing protein A {ECO:0000303|PubMed:16023795};
GN   Name=tifa {ECO:0000303|PubMed:16023795};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH TRAF6, TISSUE SPECIFICITY,
RP   MUTAGENESIS OF GLU-172, AND DEVELOPMENTAL STAGE.
RX   PubMed=16023795; DOI=10.1016/j.gene.2005.05.016;
RA   Inoue J., Yagi S., Ishikawa K., Azuma S., Ikawa S., Semba K.;
RT   "Identification and characterization of Xenopus laevis homologs of
RT   mammalian TRAF6 and its binding protein TIFA.";
RL   Gene 358:53-59(2005).
CC   -!- FUNCTION: Adapter molecule that plays a key role in the activation of
CC       pro-inflammatory NF-kappa-B signaling following detection of bacterial
CC       pathogen-associated molecular pattern metabolites (PAMPs) (By
CC       similarity). Promotes activation of an innate immune response by
CC       inducing the oligomerization and polyubiquitination of TRAF6, which
CC       leads to the activation of TAK1 and IKK through a proteasome-
CC       independent mechanism (PubMed:16023795). {ECO:0000250|UniProtKB:Q96CG3,
CC       ECO:0000269|PubMed:16023795}.
CC   -!- SUBUNIT: Interacts with traf6. {ECO:0000269|PubMed:16023795}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96CG3}.
CC   -!- TISSUE SPECIFICITY: Highly expressed in spleen and moderately expressed
CC       in ovary. {ECO:0000269|PubMed:16023795}.
CC   -!- DEVELOPMENTAL STAGE: First detected in the neurula stage and then
CC       increased and almost reached a plateau during early tailbud stage.
CC       {ECO:0000269|PubMed:16023795}.
CC   -!- SIMILARITY: Belongs to the TIFA family. {ECO:0000305}.
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DR   EMBL; AB100961; BAE78590.1; -; mRNA.
DR   RefSeq; NP_001089151.1; NM_001095682.1.
DR   RefSeq; XP_018094693.1; XM_018239204.1.
DR   AlphaFoldDB; Q2MHQ9; -.
DR   SMR; Q2MHQ9; -.
DR   GeneID; 734184; -.
DR   KEGG; xla:734184; -.
DR   CTD; 734184; -.
DR   Xenbase; XB-GENE-951835; tifa.S.
DR   OMA; SIRFFNR; -.
DR   OrthoDB; 1381983at2759; -.
DR   Proteomes; UP000186698; Chromosome 1S.
DR   Bgee; 734184; Expressed in spleen and 19 other tissues.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0044198; F:zf-TRAF domain binding; IPI:UniProtKB.
DR   GO; GO:0007250; P:activation of NF-kappaB-inducing kinase activity; IDA:UniProtKB.
DR   GO; GO:0002753; P:cytoplasmic pattern recognition receptor signaling pathway; ISS:UniProtKB.
DR   GO; GO:0007249; P:I-kappaB kinase/NF-kappaB signaling; IEA:InterPro.
DR   GO; GO:0045087; P:innate immune response; ISS:UniProtKB.
DR   GO; GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB signaling; ISS:UniProtKB.
DR   GO; GO:0051260; P:protein homooligomerization; ISS:UniProtKB.
DR   CDD; cd00060; FHA; 1.
DR   InterPro; IPR000253; FHA_dom.
DR   InterPro; IPR008984; SMAD_FHA_dom_sf.
DR   InterPro; IPR033621; TIFA.
DR   PANTHER; PTHR31266; PTHR31266; 1.
DR   Pfam; PF00498; FHA; 1.
DR   SUPFAM; SSF49879; SSF49879; 1.
DR   PROSITE; PS50006; FHA_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Immunity; Innate immunity; Reference proteome.
FT   CHAIN           1..178
FT                   /note="TRAF-interacting protein with FHA domain-containing
FT                   protein A"
FT                   /id="PRO_0000391616"
FT   DOMAIN          49..105
FT                   /note="FHA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00086"
FT   MUTAGEN         172
FT                   /note="E->A: Loss of interaction with traf6."
FT                   /evidence="ECO:0000269|PubMed:16023795"
SQ   SEQUENCE   178 AA;  20683 MW;  F243A5F56C683D23 CRC64;
     MDKELIDVDT EQTISCLTLT LYHPNHQDQK LFRGINFSRK EEIKADAVVA FGRDYNVCRY
     PLLSNRVSRI QFNLQLFKHF NCSTSTFEIK NLSKKNKLYV DNLELDYLNK IELPKKCMIR
     FGDFQILAEI DEGDSDEKFE ICCEKSHVSL VQDSFIPTMQ PIPECGTLNA VEIDENEC