ID   TIG_CAUVN               Reviewed;         452 AA.
AC   B8GX17; O87705;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 2.
DT   03-AUG-2022, entry version 87.
DE   RecName: Full=Trigger factor;
DE            Short=TF;
DE            EC=5.2.1.8;
DE   AltName: Full=PPIase;
GN   Name=tig; OrderedLocusNames=CCNA_02042;
OS   Caulobacter vibrioides (strain NA1000 / CB15N) (Caulobacter crescentus).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Caulobacterales;
OC   Caulobacteraceae; Caulobacter.
OX   NCBI_TaxID=565050;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NA1000 / CB15N;
RX   PubMed=20472802; DOI=10.1128/jb.00255-10;
RA   Marks M.E., Castro-Rojas C.M., Teiling C., Du L., Kapatral V.,
RA   Walunas T.L., Crosson S.;
RT   "The genetic basis of laboratory adaptation in Caulobacter crescentus.";
RL   J. Bacteriol. 192:3678-3688(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-452.
RA   Jenal U., Fuchs T.;
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in protein export. Acts as a chaperone by
CC       maintaining the newly synthesized protein in an open conformation.
CC       Functions as a peptidyl-prolyl cis-trans isomerase (By similarity).
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[protein]-peptidylproline (omega=180) = [protein]-
CC         peptidylproline (omega=0); Xref=Rhea:RHEA:16237, Rhea:RHEA-
CC         COMP:10747, Rhea:RHEA-COMP:10748, ChEBI:CHEBI:83833,
CC         ChEBI:CHEBI:83834; EC=5.2.1.8;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Note=About half TF is bound to the
CC       ribosome near the polypeptide exit tunnel while the other half is free
CC       in the cytoplasm. {ECO:0000250}.
CC   -!- DOMAIN: Consists of 3 domains; the N-terminus binds the ribosome, the
CC       middle domain has PPIase activity, while the C-terminus has intrinsic
CC       chaperone activity on its own. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the FKBP-type PPIase family. Tig subfamily.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ACL95507.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP001340; ACL95507.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AJ010321; CAA09089.1; -; Genomic_DNA.
DR   RefSeq; YP_002517415.1; NC_011916.1.
DR   AlphaFoldDB; B8GX17; -.
DR   SMR; B8GX17; -.
DR   PRIDE; B8GX17; -.
DR   EnsemblBacteria; ACL95507; ACL95507; CCNA_02042.
DR   GeneID; 7333373; -.
DR   KEGG; ccs:CCNA_02042; -.
DR   PATRIC; fig|565050.3.peg.2000; -.
DR   HOGENOM; CLU_033058_2_2_5; -.
DR   OMA; KGIKTQF; -.
DR   OrthoDB; 932993at2; -.
DR   PhylomeDB; B8GX17; -.
DR   Proteomes; UP000001364; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003755; F:peptidyl-prolyl cis-trans isomerase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0006457; P:protein folding; IEA:UniProtKB-UniRule.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.10.3120.10; -; 1.
DR   Gene3D; 3.10.50.40; -; 1.
DR   Gene3D; 3.30.70.1050; -; 1.
DR   HAMAP; MF_00303; Trigger_factor_Tig; 1.
DR   InterPro; IPR046357; PPIase_dom_sf.
DR   InterPro; IPR001179; PPIase_FKBP_dom.
DR   InterPro; IPR005215; Trig_fac.
DR   InterPro; IPR008880; Trigger_fac_C.
DR   InterPro; IPR037041; Trigger_fac_C_sf.
DR   InterPro; IPR008881; Trigger_fac_ribosome-bd_bac.
DR   InterPro; IPR036611; Trigger_fac_ribosome-bd_sf.
DR   InterPro; IPR027304; Trigger_fact/SurA_dom_sf.
DR   PANTHER; PTHR30560; PTHR30560; 1.
DR   Pfam; PF00254; FKBP_C; 1.
DR   Pfam; PF05698; Trigger_C; 1.
DR   Pfam; PF05697; Trigger_N; 1.
DR   PIRSF; PIRSF003095; Trigger_factor; 1.
DR   SUPFAM; SSF102735; SSF102735; 1.
DR   SUPFAM; SSF109998; SSF109998; 1.
DR   TIGRFAMs; TIGR00115; tig; 1.
DR   PROSITE; PS50059; FKBP_PPIASE; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Chaperone; Cytoplasm; Isomerase;
KW   Reference proteome; Rotamase.
FT   CHAIN           1..452
FT                   /note="Trigger factor"
FT                   /id="PRO_0000378313"
FT   DOMAIN          169..254
FT                   /note="PPIase FKBP-type"
FT   CONFLICT        215
FT                   /note="P -> R (in Ref. 2; CAA09089)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   452 AA;  49767 MW;  5C05DB6A4B2D4D94 CRC64;
     MQIVEKSGEG LSRVFGVTVP ASELATRLEA RIAEVAPQMN VKGFRPGKVP TAHVRRLYGK
     ALMGEVIEQA LNETTTKVLE DNKLRPAGQP ELNPSSDMDK VIAGGEDLSF DLAVEVMPEF
     EPIDPTSIEL VKPVYKVSDE EVQEALDELA KQARTYEPRT GKSLKAKDGD QLLIDFVGTI
     DGVEFAGGKA EGAELVLGSG QFIPGFEDQL VGAKPGDDVV VKVKFPEEYQ AKDLAGKDAE
     FATKVQEVRA PVDGKADDEL AKRLGLSDLA ALTELLKSNL AGRYDNSSRF KLKRALLDVL
     DTKHDFPLPP RMVDAEFAGI WQQVEADKAR GGLPPEDAEK TEDQLKDEYK KIAERRVRLG
     LVLAEIGRKN DVVVTDQELT DAIMREARQY GAQAQQVFDM YRQRADLQAA LRAPIYEDKV
     VDLIFGKAKI EEKEVSKDEL LEEDDLPEGY GG