BSC5_YEAST
ID BSC5_YEAST Reviewed; 489 AA.
AC P53755; D6W1P4; Q66R20;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Bypass of stop codon protein 5;
GN Name=BSC5; OrderedLocusNames=YNR069C; ORFNames=N3555;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169873;
RA Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K.,
RA Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K.,
RA Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M.,
RA Beinhauer J.D., Boskovic J., Buitrago M.J., Bussereau F., Coster F.,
RA Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F.,
RA Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C.,
RA Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A.,
RA Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H.,
RA Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L.,
RA Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R.,
RA Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D.,
RA Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A.,
RA Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C.,
RA Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F.,
RA Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G.,
RA Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M.,
RA Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its
RT evolutionary implications.";
RL Nature 387:93-98(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP GENE NAME.
RX PubMed=12711673; DOI=10.1093/nar/gkg330;
RA Namy O., Duchateau-Nguyen G., Hatin I., Hermann-Le Denmat S., Termier M.,
RA Rousset J.-P.;
RT "Identification of stop codon readthrough genes in Saccharomyces
RT cerevisiae.";
RL Nucleic Acids Res. 31:2289-2296(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-111 AND SER-350, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: May be a component of the ubiquitination pathway.
CC {ECO:0000250}.
CC -!- MISCELLANEOUS: Open reading frame exhibits genomic organization
CC compatible with a translational readthrough-dependent mode of
CC expression.
CC -!- SIMILARITY: Belongs to the BUL1 family. {ECO:0000305}.
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DR EMBL; Z71684; CAA96351.1; -; Genomic_DNA.
DR EMBL; AY723863; AAU09780.1; -; Genomic_DNA.
DR EMBL; BK006947; DAA10610.1; -; Genomic_DNA.
DR PIR; S63401; S63401.
DR RefSeq; NP_014467.3; NM_001183246.3.
DR AlphaFoldDB; P53755; -.
DR BioGRID; 35895; 22.
DR DIP; DIP-1400N; -.
DR IntAct; P53755; 4.
DR MINT; P53755; -.
DR STRING; 4932.YNR069C; -.
DR iPTMnet; P53755; -.
DR MaxQB; P53755; -.
DR PaxDb; P53755; -.
DR PRIDE; P53755; -.
DR EnsemblFungi; YNR069C_mRNA; YNR069C; YNR069C.
DR GeneID; 855806; -.
DR KEGG; sce:YNR069C; -.
DR SGD; S000005352; BSC5.
DR VEuPathDB; FungiDB:YNR069C; -.
DR eggNOG; ENOG502QSAC; Eukaryota.
DR GeneTree; ENSGT00940000176311; -.
DR HOGENOM; CLU_631849_0_0_1; -.
DR OMA; EHESPRN; -.
DR BioCyc; YEAST:G3O-33373-MON; -.
DR PRO; PR:P53755; -.
DR Proteomes; UP000002311; Chromosome XIV.
DR RNAct; P53755; protein.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0034450; F:ubiquitin-ubiquitin ligase activity; IBA:GO_Central.
DR GO; GO:0006513; P:protein monoubiquitination; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR InterPro; IPR039634; Bul1-like.
DR InterPro; IPR007519; Bul1_N.
DR PANTHER; PTHR31904; PTHR31904; 1.
DR Pfam; PF04425; Bul1_N; 1.
PE 1: Evidence at protein level;
KW Phosphoprotein; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..489
FT /note="Bypass of stop codon protein 5"
FT /id="PRO_0000064995"
FT REGION 1..42
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 11..35
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 111
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 350
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 209
FT /note="Y -> N (in Ref. 3; AAU09780)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 489 AA; 54831 MW; D2657B95F1CAF824 CRC64;
MQESKEPQNK FEGCQRISSS SSTLFGGTSF EEPRCGTSQG KEEDAFACNN GDHCSSITNV
QEDDFVLPEL LPSFEMYENL LSNIPQSSFD TYFPENPPFY EVASRNQSIP SEGESGNDMR
ILTGDIVGPD NHEVTVDGRR FASGPAESQI RNYDDTKGIP VENIYALPRI KTPIATELYV
TKTAPKFGQL PKHESMLREY TSGDIIHGYF TVENKSTKPI KFDMFYLTLE GTTSSKTQSP
FGIQKTTERI LRMVDMAASW SYNHEDVNTG EDLCGFFDSI DKTSFGLPNS RILNPGDKRK
KFFTFKIPNQ LLDVTCKHGH FSHSLLPPTL GFDRPSSSHP ELSTLKFSES LGYGRLSERG
SSLWLNDSSS GSLINYSINA MIVGKDVASG RVCLMSEKKY SIRIVPFGFQ NNPISREKCL
KDLEDFDIEI ANRLGMIEKV FSKIERAIPI HKEDIQEANR SDQLSPLRGK YEWNAVAGNT
ENGTLKKKH
