TILB_DROME
ID TILB_DROME Reviewed; 395 AA.
AC Q9VR52;
DT 25-JAN-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Protein tilB;
DE AltName: Full=Touch insensitive larva B protein;
GN Name=tilB; ORFNames=CG14620;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20215474; DOI=10.1534/genetics.110.114009;
RA Kavlie R.G., Kernan M.J., Eberl D.F.;
RT "Hearing in Drosophila requires TilB, a conserved protein associated with
RT ciliary motility.";
RL Genetics 185:177-188(2010).
CC -!- FUNCTION: Cilium-specific protein required for cilia structures in
CC Johnston's sensory organ. Plays a role in motility of cilia and
CC flagella. {ECO:0000269|PubMed:20215474}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:20215474}. Cell
CC projection, dendrite {ECO:0000269|PubMed:20215474}. Cell projection,
CC cilium, flagellum {ECO:0000269|PubMed:20215474}. Note=Detected in the
CC inner and outer dendritic segment and basal body.
CC -!- TISSUE SPECIFICITY: Expressed in chordotonal sense organs, Johnston's
CC organ and sperm (at protein level). Expressed in ciliated cells.
CC Expressed in the head and testis. {ECO:0000269|PubMed:20215474}.
CC -!- DEVELOPMENTAL STAGE: Expressed in ciliated cells of the chordotonal
CC organs, neurons of Johnston's organ and femoral chordotonal organs,
CC olfactory sensilla on the antenna in late stage embryos and larvae.
CC {ECO:0000269|PubMed:20215474}.
CC -!- DISRUPTION PHENOTYPE: Larvae mutants are insensitive to touch and have
CC defects in larval locomotion. Adult mutants show defects in motility
CC and coordination and are deaf; lack antenna oscillations and mechanical
CC amplification of stimulus-induced antennal vibrations. Mutant males can
CC mate but are sterile because of amotile sperm; tail axonemes lacked the
CC inner dynein arms. {ECO:0000269|PubMed:20215474}.
CC -!- SIMILARITY: Belongs to the tilB family. {ECO:0000305}.
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DR EMBL; AE014298; AAF50954.1; -; Genomic_DNA.
DR RefSeq; NP_608460.1; NM_134616.3.
DR AlphaFoldDB; Q9VR52; -.
DR SMR; Q9VR52; -.
DR BioGRID; 59404; 2.
DR IntAct; Q9VR52; 2.
DR STRING; 7227.FBpp0077037; -.
DR PaxDb; Q9VR52; -.
DR EnsemblMetazoa; FBtr0077345; FBpp0077037; FBgn0014395.
DR GeneID; 33130; -.
DR KEGG; dme:Dmel_CG14620; -.
DR UCSC; CG14620-RA; d. melanogaster.
DR CTD; 33130; -.
DR FlyBase; FBgn0014395; tilB.
DR VEuPathDB; VectorBase:FBgn0014395; -.
DR eggNOG; KOG0531; Eukaryota.
DR GeneTree; ENSGT00940000158506; -.
DR HOGENOM; CLU_034806_0_1_1; -.
DR InParanoid; Q9VR52; -.
DR OMA; QHRAVIV; -.
DR OrthoDB; 800324at2759; -.
DR PhylomeDB; Q9VR52; -.
DR BioGRID-ORCS; 33130; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 33130; -.
DR PRO; PR:Q9VR52; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0014395; Expressed in testis and 10 other tissues.
DR ExpressionAtlas; Q9VR52; baseline and differential.
DR Genevisible; Q9VR52; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0030425; C:dendrite; IDA:UniProtKB.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0035082; P:axoneme assembly; IMP:FlyBase.
DR GO; GO:0060285; P:cilium-dependent cell motility; IMP:UniProtKB.
DR GO; GO:0045433; P:male courtship behavior, veined wing generated song production; IMP:FlyBase.
DR GO; GO:0036158; P:outer dynein arm assembly; IBA:GO_Central.
DR GO; GO:0007605; P:sensory perception of sound; IMP:FlyBase.
DR GO; GO:0010378; P:temperature compensation of the circadian clock; IMP:FlyBase.
DR Gene3D; 3.80.10.10; -; 1.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR032675; LRR_dom_sf.
DR PROSITE; PS51450; LRR; 5.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Coiled coil; Cytoplasm; Flagellum;
KW Leucine-rich repeat; Reference proteome; Repeat.
FT CHAIN 1..395
FT /note="Protein tilB"
FT /id="PRO_0000414861"
FT REPEAT 20..44
FT /note="LRR 1"
FT REPEAT 46..65
FT /note="LRR 2"
FT REPEAT 66..89
FT /note="LRR 3"
FT REPEAT 90..110
FT /note="LRR 4"
FT DOMAIN 128..146
FT /note="LRRCT"
FT DOMAIN 264..355
FT /note="CS"
FT COILED 170..196
FT /evidence="ECO:0000255"
SQ SEQUENCE 395 AA; 45608 MW; 069201406B0083F5 CRC64;
MVLITEELVR KKSEHNERLI STLEEISLHQ EDIEVIEHIQ NWCRDLKILL LQSNLIARLE
NLHKLKRLEY LNVAINNIER VENLEGCESL SKLDLTLNFI RELTSVESLC GNYNLRELVL
IGNPCVDYPH YRDYVVATLP QLNSLDCVEI TPSERLRALR ELSKNRSIIV QKQVEQDIER
DEQRIRVAKQ QSALAEHCAG IEDEEERIKA FWQAKSEHCP EIRTEIARQH RLGRERHETK
SPLDPLKPQR NLFAPCGRPY NLNQAKLPFK FRDEADHYLL QLEVYRHLDT SLIDVDVQTT
YTRVTVKKKI FQIAYSEEVK PDESTVQRSQ ITGHLVVNLK KLKVNELLIA KKSPTKSPAA
PFDAGKKDGK PEEAFHGGVV DISNICAPED LPDLI
