ID   BSHC_BACLD              Reviewed;         538 AA.
AC   Q65JZ0; Q62VE1;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 88.
DE   RecName: Full=Putative cysteine ligase BshC {ECO:0000255|HAMAP-Rule:MF_01867};
DE            EC=6.-.-.- {ECO:0000255|HAMAP-Rule:MF_01867};
GN   Name=bshC {ECO:0000255|HAMAP-Rule:MF_01867};
GN   OrderedLocusNames=BLi01729, BL00850;
OS   Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 /
OS   NBRC 12200 / NCIMB 9375 / NCTC 10341 / NRRL NRS-1264 / Gibson 46).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=279010;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15383718; DOI=10.1159/000079829;
RA   Veith B., Herzberg C., Steckel S., Feesche J., Maurer K.H., Ehrenreich P.,
RA   Baeumer S., Henne A., Liesegang H., Merkl R., Ehrenreich A., Gottschalk G.;
RT   "The complete genome sequence of Bacillus licheniformis DSM13, an organism
RT   with great industrial potential.";
RL   J. Mol. Microbiol. Biotechnol. 7:204-211(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14580 / DSM 13 / JCM 2505 / CCUG 7422 / NBRC 12200 / NCIMB 9375
RC   / NCTC 10341 / NRRL NRS-1264 / Gibson 46;
RX   PubMed=15461803; DOI=10.1186/gb-2004-5-10-r77;
RA   Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J.,
RA   Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B.,
RA   Rasmussen M.D., Andersen J.T., Joergensen P.L., Larsen T.S., Sorokin A.,
RA   Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M.;
RT   "Complete genome sequence of the industrial bacterium Bacillus
RT   licheniformis and comparisons with closely related Bacillus species.";
RL   Genome Biol. 5:R77.1-R77.12(2004).
CC   -!- FUNCTION: Involved in bacillithiol (BSH) biosynthesis. May catalyze the
CC       last step of the pathway, the addition of cysteine to glucosamine
CC       malate (GlcN-Mal) to generate BSH. {ECO:0000255|HAMAP-Rule:MF_01867}.
CC   -!- SIMILARITY: Belongs to the BshC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01867}.
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DR   EMBL; CP000002; AAU23267.2; -; Genomic_DNA.
DR   EMBL; AE017333; AAU40624.1; -; Genomic_DNA.
DR   RefSeq; WP_011197956.1; NC_006322.1.
DR   AlphaFoldDB; Q65JZ0; -.
DR   SMR; Q65JZ0; -.
DR   STRING; 279010.BL00850; -.
DR   EnsemblBacteria; AAU23267; AAU23267; BL00850.
DR   KEGG; bld:BLi01729; -.
DR   KEGG; bli:BL00850; -.
DR   PATRIC; fig|279010.13.peg.1728; -.
DR   eggNOG; COG4365; Bacteria.
DR   HOGENOM; CLU_022249_1_0_9; -.
DR   OMA; TTGHQLN; -.
DR   OrthoDB; 295429at2; -.
DR   BioCyc; BLIC279010:BLI_RS08550-MON; -.
DR   Proteomes; UP000000606; Chromosome.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01867; BshC; 1.
DR   InterPro; IPR011199; Bacillithiol_biosynth_BshC.
DR   Pfam; PF10079; BshC; 1.
DR   PIRSF; PIRSF012535; UCP012535; 1.
DR   TIGRFAMs; TIGR03998; thiol_BshC; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Ligase; Reference proteome.
FT   CHAIN           1..538
FT                   /note="Putative cysteine ligase BshC"
FT                   /id="PRO_0000378219"
FT   COILED          454..482
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01867"
SQ   SEQUENCE   538 AA;  62300 MW;  928D57F8F4FC3553 CRC64;
     MQLTELSIQS QNPFVRDYIN GKKEIEPFFD YGLSNESWSV RLDDLSSRTY DRDALADYLL
     DYHRKFQSAT MNETIERLRD PKSVVVVGGQ QAGLLTGPLY TIHKIVSILL LAKQKEQELN
     VPVIPVFWVA GEDHDLEEIN HVYISDGGKV KKHKLPQSHW KKSQAAKTAL DAEKAEKWLD
     GIFASFEETE YTNDLLGHLR RCLRQSLSFT DFFEFLVADM FEQDGLILLN SGDPGIRNLE
     ARFFRQLLDK NDELTDSVKR QQELMKQLGY TPIIEGAAQH ANIFYERDGE RFLIEKENGA
     FFIKELHLQW SEAELCDLIC QNPEAFSNNV VTRPLMQEYL LPTLAFIAGP GEINYWGELK
     GAFQVMGYKM PPVVPRLQVT FLERHIEKKL DERGIELRES IEKGARAKKE QYFQDKVPSG
     FTDSVKQAKE KIENIHSAVR AEALEIDGSL GPLLEKNAGF IQDQLQFLEK TVIRRIEEKE
     NYILRDFDKI QTSIKPLDAP QERIWNIVYY LNKYGPDFLE KYKDLPYSFQ NMHQVVKL