ID   BSHC_BACP2              Reviewed;         539 AA.
AC   A8FCX1;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2007, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Putative cysteine ligase BshC {ECO:0000255|HAMAP-Rule:MF_01867};
DE            EC=6.-.-.- {ECO:0000255|HAMAP-Rule:MF_01867};
GN   Name=bshC {ECO:0000255|HAMAP-Rule:MF_01867}; OrderedLocusNames=BPUM_1405;
OS   Bacillus pumilus (strain SAFR-032).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=315750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SAFR-032;
RX   PubMed=17895969; DOI=10.1371/journal.pone.0000928;
RA   Gioia J., Yerrapragada S., Qin X., Jiang H., Igboeli O.C., Muzny D.,
RA   Dugan-Rocha S., Ding Y., Hawes A., Liu W., Perez L., Kovar C., Dinh H.,
RA   Lee S., Nazareth L., Blyth P., Holder M., Buhay C., Tirumalai M.R., Liu Y.,
RA   Dasgupta I., Bokhetache L., Fujita M., Karouia F., Eswara Moorthy P.,
RA   Siefert J., Uzman A., Buzumbo P., Verma A., Zwiya H., McWilliams B.D.,
RA   Olowu A., Clinkenbeard K.D., Newcombe D., Golebiewski L., Petrosino J.F.,
RA   Nicholson W.L., Fox G.E., Venkateswaran K., Highlander S.K.,
RA   Weinstock G.M.;
RT   "Paradoxical DNA repair and peroxide resistance gene conservation in
RT   Bacillus pumilus SAFR-032.";
RL   PLoS ONE 2:E928-E928(2007).
CC   -!- FUNCTION: Involved in bacillithiol (BSH) biosynthesis. May catalyze the
CC       last step of the pathway, the addition of cysteine to glucosamine
CC       malate (GlcN-Mal) to generate BSH. {ECO:0000255|HAMAP-Rule:MF_01867}.
CC   -!- SIMILARITY: Belongs to the BshC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01867}.
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DR   EMBL; CP000813; ABV62088.1; -; Genomic_DNA.
DR   RefSeq; WP_012009861.1; NC_009848.4.
DR   AlphaFoldDB; A8FCX1; -.
DR   SMR; A8FCX1; -.
DR   STRING; 315750.BPUM_1405; -.
DR   EnsemblBacteria; ABV62088; ABV62088; BPUM_1405.
DR   KEGG; bpu:BPUM_1405; -.
DR   eggNOG; COG4365; Bacteria.
DR   HOGENOM; CLU_022249_1_0_9; -.
DR   OMA; TTGHQLN; -.
DR   OrthoDB; 295429at2; -.
DR   Proteomes; UP000001355; Chromosome.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01867; BshC; 1.
DR   InterPro; IPR011199; Bacillithiol_biosynth_BshC.
DR   Pfam; PF10079; BshC; 1.
DR   PIRSF; PIRSF012535; UCP012535; 1.
DR   TIGRFAMs; TIGR03998; thiol_BshC; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Ligase; Reference proteome.
FT   CHAIN           1..539
FT                   /note="Putative cysteine ligase BshC"
FT                   /id="PRO_0000378220"
FT   COILED          249..270
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01867"
SQ   SEQUENCE   539 AA;  63193 MW;  394B468D00F450E1 CRC64;
     MQLTELSIRS QNLFIRDYIE EKKEMTTFFD YDIHSEHTWK KRYDDLMKMS FPREALADYM
     SAYHAKFESA AMRQNIEKIR DKRSVMVVGG QQAGLLTGPL YTIHKIISII QFAKEKETEL
     GVPVIPVFWV AGEDHDVDEI NFVYTSGEKG PVKQKLSLHN VKKAAAKRIP LDQEKTEIWL
     RDVFSTYEES AYTNDLLVQL LRCLRKSQTF TDFFEWIVCD LFEEDGLLLF NSGDLGVKPL
     ERTLFKHIVE TNDEVTNRLN ESQAAMKRAG YQPIIEAGDN QANLFYEYDE ERFLIEKENG
     RFFISEVGLT WTKEELLKEV EEHPERFSNN VVTRPLMQET LLPTLAFMAG HGEVNYWGEL
     KGIFEHFHLK MAPVLPRLHV TILERHIDKK LPVRELSVEE VLTNGVKEKK DAHFQQSLPD
     SFVQAVEHAK RELANAHGVM RQEALEIEPN FEQLLDKNAK FIEDQLQFVY QKVAQRVEEK
     EGYILRDFER IENSLKPLDA PQERIWNIMY FLNKYGPEFF KTFKNLPFSF QNKQQIVKL