ID   TILS_CHLVU              Reviewed;         504 AA.
AC   O20163;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 71.
DE   RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic {ECO:0000255|HAMAP-Rule:MF_01161};
DE            EC=6.3.4.19 {ECO:0000255|HAMAP-Rule:MF_01161};
DE   AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase {ECO:0000255|HAMAP-Rule:MF_01161};
DE   AltName: Full=tRNA(Ile)-lysidine synthetase {ECO:0000255|HAMAP-Rule:MF_01161};
GN   Name=tilS {ECO:0000255|HAMAP-Rule:MF_01161}; Synonyms=ycf62;
OS   Chlorella vulgaris (Green alga).
OG   Plastid; Chloroplast.
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Trebouxiophyceae;
OC   Chlorellales; Chlorellaceae; Chlorella clade; Chlorella.
OX   NCBI_TaxID=3077;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IAM C-27 / Tamiya;
RX   PubMed=9159184; DOI=10.1073/pnas.94.11.5967;
RA   Wakasugi T., Nagai T., Kapoor M., Sugita M., Ito M., Ito S., Tsudzuki J.,
RA   Nakashima K., Tsudzuki T., Suzuki Y., Hamada A., Ohta T., Inamura A.,
RA   Yoshinaga K., Sugiura M.;
RT   "Complete nucleotide sequence of the chloroplast genome from the green alga
RT   Chlorella vulgaris: the existence of genes possibly involved in chloroplast
RT   division.";
RL   Proc. Natl. Acad. Sci. U.S.A. 94:5967-5972(1997).
CC   -!- FUNCTION: Ligates lysine onto the cytidine present at position 34 of
CC       the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an
CC       ATP-dependent manner. Cytidine is converted to lysidine, thus changing
CC       the amino acid specificity of the tRNA from methionine to isoleucine.
CC       {ECO:0000255|HAMAP-Rule:MF_01161}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + cytidine(34) in tRNA(Ile2) + L-lysine = AMP +
CC         diphosphate + H(+) + lysidine(34) in tRNA(Ile2);
CC         Xref=Rhea:RHEA:43744, Rhea:RHEA-COMP:10625, Rhea:RHEA-COMP:10670,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:32551,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:82748, ChEBI:CHEBI:83665,
CC         ChEBI:CHEBI:456215; EC=6.3.4.19; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01161};
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- DOMAIN: The N-terminal region contains the highly conserved SGGXDS
CC       motif, predicted to be a P-loop motif involved in ATP binding.
CC   -!- SIMILARITY: Belongs to the tRNA(Ile)-lysidine synthase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01161}.
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DR   EMBL; AB001684; BAA57935.1; -; Genomic_DNA.
DR   PIR; T07287; T07287.
DR   RefSeq; NP_045859.1; NC_001865.1.
DR   AlphaFoldDB; O20163; -.
DR   PRIDE; O20163; -.
DR   GeneID; 1457443; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016879; F:ligase activity, forming carbon-nitrogen bonds; IEA:UniProtKB-UniRule.
DR   GO; GO:0002097; P:tRNA wobble base modification; IEA:UniProt.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_01161; tRNA_Ile_lys_synt; 1.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR011063; TilS/TtcA_N.
DR   InterPro; IPR012094; tRNA_Ile_lys_synt.
DR   Pfam; PF01171; ATP_bind_3; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Chloroplast; Ligase; Nucleotide-binding; Plastid;
KW   tRNA processing.
FT   CHAIN           1..504
FT                   /note="tRNA(Ile)-lysidine synthase, chloroplastic"
FT                   /id="PRO_0000181815"
FT   BINDING         35..40
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01161"
SQ   SEQUENCE   504 AA;  59973 MW;  E2A4FD28192C6AF0 CRC64;
     MKKDSTIIST CNEFSLSSVK TKRVYKNNHL LCSVSGGQDS IVSFFFLVNF LYLSKNFKLI
     KSKLSSFSVA SKSAEKKRCF DCFFSLKKNK KINALINKEK KLSRLDLVFC QHFWQTQNFF
     CVEFLFQLTF FFDIPYTFVL SKNILVSENR ARGWRKKTFS RLLKIQKLST IVTGQTKTDT
     AEKSITNLLR GTSPKSFSLI TKANSKTTSS FFCFRLFRPK TFYLTLTGNK RERKVNRRIS
     FSFYRTQKKD SKKLRETRAP FFIEMASFSA FFGNTFQKKK SSNSFLLYKT RLSFSFCFSG
     ETREHSINSL KPLQNRNRYH ISKLVKFYKF PLTIDATNFS LNFSRNKIRH LFIPFIRFLF
     KLKFETLLLN FLYLVNLEHE QIENENLELK RVLNFLRINY LKKNFFSSNR RSFFVIQTGH
     KNNNVFPTKK KEKRWFYCNE IGISKIRKQC KIHEDPILET SLVIYFTSVL TTSRKYSLLQ
     NLLYNYREIE INFRQISKLE NSFW