ID   TIM10_DANRE             Reviewed;          88 AA.
AC   Q6DI06;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10;
GN   Name=timm10; Synonyms=tim10; ORFNames=zgc:86900;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC       the import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. May also be required for the transfer of
CC       beta-barrel precursors from the TOM complex to the sorting and assembly
CC       machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC       protein that protects the hydrophobic precursors from aggregation and
CC       guide them through the mitochondrial intermembrane space (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of TIMM9 and 3 copies of
CC       TIMM10/TIM10A, named soluble 70 kDa complex. The complex forms a 6-
CC       bladed alpha-propeller structure and associates with the TIMM22
CC       component of the TIM22 complex. Interacts with multi-pass transmembrane
CC       proteins in transit (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC       {ECO:0000250}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of TIMM10 from cytoplasm into mitochondrion, the Cys
CC       residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC075785; AAH75785.1; -; mRNA.
DR   RefSeq; NP_001002678.1; NM_001002678.3.
DR   AlphaFoldDB; Q6DI06; -.
DR   SMR; Q6DI06; -.
DR   STRING; 7955.ENSDARP00000091094; -.
DR   PaxDb; Q6DI06; -.
DR   Ensembl; ENSDART00000100321; ENSDARP00000091094; ENSDARG00000069116.
DR   GeneID; 436951; -.
DR   KEGG; dre:436951; -.
DR   CTD; 26519; -.
DR   ZFIN; ZDB-GENE-040718-427; timm10.
DR   eggNOG; KOG3480; Eukaryota.
DR   GeneTree; ENSGT00390000003068; -.
DR   HOGENOM; CLU_162151_2_0_1; -.
DR   InParanoid; Q6DI06; -.
DR   OMA; ELEIEMM; -.
DR   OrthoDB; 1477334at2759; -.
DR   PhylomeDB; Q6DI06; -.
DR   TreeFam; TF106193; -.
DR   Reactome; R-DRE-1268020; Mitochondrial protein import.
DR   PRO; PR:Q6DI06; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 1.
DR   Bgee; ENSDARG00000069116; Expressed in tail and 34 other tissues.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR027100; Tim10.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   PANTHER; PTHR11038:SF16; PTHR11038:SF16; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..88
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10"
FT                   /id="PRO_0000228054"
FT   MOTIF           29..54
FT                   /note="Twin CX3C motif"
FT   DISULFID        29..54
FT                   /evidence="ECO:0000250"
FT   DISULFID        33..50
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   88 AA;  9956 MW;  E1D7CF07A4733559 CRC64;
     MDPMKAQQLA AELEVEMMAD MYNRMTNACH RKCVPPHYKE AELSKGEAVC LDRCVAKYLD
     LHERLGRKLT ELSVQDEEVM RKAAAGTG