ID   TIM10_DEBHA             Reviewed;          91 AA.
AC   Q6BHJ3;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 107.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM10;
GN   Name=TIM10; OrderedLocusNames=DEHA2G18106g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC       the import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. Also required for the transfer of beta-
CC       barrel precursors from the TOM complex to the sorting and assembly
CC       machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC       protein that protects the hydrophobic precursors from aggregation and
CC       guide them through the mitochondrial intermembrane space (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of TIM9 and 3 copies of
CC       TIM10, named soluble 70 kDa complex. Associates directly with the TIM22
CC       complex, whose core is composed of TIM22 and TIM54. Interacts with the
CC       transmembrane regions of multi-pass transmembrane proteins in transit
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Intermembrane side
CC       {ECO:0000250}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of TIM10 from cytoplasm into mitochondrion, the Cys
CC       residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; CR382139; CAG90834.1; -; Genomic_DNA.
DR   RefSeq; XP_462328.1; XM_462328.1.
DR   AlphaFoldDB; Q6BHJ3; -.
DR   SMR; Q6BHJ3; -.
DR   STRING; 4959.XP_462328.1; -.
DR   EnsemblFungi; CAG90834; CAG90834; DEHA2G18106g.
DR   GeneID; 2905266; -.
DR   KEGG; dha:DEHA2G18106g; -.
DR   VEuPathDB; FungiDB:DEHA2G18106g; -.
DR   eggNOG; KOG3480; Eukaryota.
DR   HOGENOM; CLU_162151_1_0_1; -.
DR   InParanoid; Q6BHJ3; -.
DR   OMA; ELEIEMM; -.
DR   OrthoDB; 1477334at2759; -.
DR   Proteomes; UP000000599; Chromosome G.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:InterPro.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR027100; Tim10.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   PANTHER; PTHR11038:SF16; PTHR11038:SF16; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..91
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM10"
FT                   /id="PRO_0000193617"
FT   MOTIF           38..63
FT                   /note="Twin CX3C motif"
FT   DISULFID        38..63
FT                   /evidence="ECO:0000250"
FT   DISULFID        42..59
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   91 AA;  10108 MW;  33E0A59CA8D9ED4B CRC64;
     MFGLGGAAPQ ISSQQKLQAA EAELDMVTGM FNQLVEQCHS KCINKTYNDS EVSKQEALCL
     DRCVAKYFET NVQVGEHMQK MGQSGQFMGR Q