ID   TIM10_DICDI             Reviewed;          88 AA.
AC   Q54NZ0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 97.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10;
GN   Name=timm10; Synonyms=tim10; ORFNames=DDB_G0284911;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the TIM22 complex, a complex that mediates the
CC       import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. The TIM22 complex forms a twin-pore
CC       translocase that uses the membrane potential as external driving force
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of timm9 and 3 copies of
CC       timm10, named soluble 70 kDa complex. Associates directly with the
CC       TIM22 complex, whose core is composed of timm22. Interacts with the
CC       transmembrane regions of multi-pass transmembrane proteins in transit
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of timm9/timm10 from cytoplasm into mitochondrion,
CC       the Cys residues probably coordinate zinc, thereby preventing folding
CC       and allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; AAFI02000073; EAL64919.1; -; Genomic_DNA.
DR   RefSeq; XP_639927.1; XM_634835.1.
DR   AlphaFoldDB; Q54NZ0; -.
DR   SMR; Q54NZ0; -.
DR   STRING; 44689.DDB0252758; -.
DR   PaxDb; Q54NZ0; -.
DR   EnsemblProtists; EAL64919; EAL64919; DDB_G0284911.
DR   GeneID; 8624839; -.
DR   KEGG; ddi:DDB_G0284911; -.
DR   dictyBase; DDB_G0284911; timm10.
DR   eggNOG; ENOG502RICN; Eukaryota.
DR   HOGENOM; CLU_2473661_0_0_1; -.
DR   InParanoid; Q54NZ0; -.
DR   OMA; CSAKCIS; -.
DR   PhylomeDB; Q54NZ0; -.
DR   PRO; PR:Q54NZ0; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IBA:GO_Central.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; ISS:dictyBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:dictyBase.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR027100; Tim10.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   PANTHER; PTHR11038:SF16; PTHR11038:SF16; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..88
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10"
FT                   /id="PRO_0000331559"
FT   REGION          63..88
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           25..49
FT                   /note="Twin CX3C motif"
FT   DISULFID        25..49
FT                   /evidence="ECO:0000250"
FT   DISULFID        29..45
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   88 AA;  9855 MW;  465AA8CFEBB632C8 CRC64;
     MDDVEMKVME MKMISKMFQG ILDACSAKCI SKYNEGDLNV GESVCAERCV QKWMETFKKV
     QSKMSGTQPG QEVPQEAPAA APEKKGWF