ID   TIM10_PICSO             Reviewed;          90 AA.
AC   Q9P335; G8YH61;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim10;
GN   Name=TIM10;
GN   ORFNames=GNLVRS01_PISO0G04758g, GNLVRS01_PISO0H04759g, Piso0_003094;
OS   Pichia sorbitophila (strain ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC
OS   10061 / NRRL Y-12695) (Hybrid yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Millerozyma.
OX   NCBI_TaxID=559304;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695;
RX   PubMed=10806421;
RX   DOI=10.1002/(sici)1097-0061(200005)16:7<589::aid-yea555>3.0.co;2-g;
RA   Kayingo G., Potier S., Hohmann S., Prior B.A.;
RT   "Isolation and characterisation of the putative TIM10 homologue from the
RT   yeast Pichia sorbitophila: a putative component of the mitochondrial
RT   protein import system.";
RL   Yeast 16:589-596(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC MYA-4447 / BCRC 22081 / CBS 7064 / NBRC 10061 / NRRL Y-12695;
RX   DOI=10.1534/g3.111.000745;
RA   Leh Louis V., Despons L., Friedrich A., Martin T., Durrens P.,
RA   Casaregola S., Neuveglise C., Fairhead C., Marck C., Cruz J.A.,
RA   Straub M.-L., Kugler V., Sacerdot C., Uzunov Z., Thierry A., Weiss S.,
RA   Bleykasten C., De Montigny J., Jacques N., Jung P., Lemaire M., Mallet S.,
RA   Morel G., Richard G.-F., Sarkar A., Savel G., Schacherer J., Seret M.-L.,
RA   Talla E., Samson G., Jubin C., Poulain J., Vacherie B., Barbe V.,
RA   Pelletier E., Sherman D.J., Westhof E., Weissenbach J., Baret P.V.,
RA   Wincker P., Gaillardin C., Dujon B., Souciet J.-L.;
RT   "Pichia sorbitophila, an interspecies yeast hybrid reveals early steps of
RT   genome resolution following polyploidization.";
RL   G3 (Bethesda) 2:299-311(2012).
CC   -!- FUNCTION: Mitochondrial intermembrane chaperone that participates in
CC       the import and insertion of multi-pass transmembrane proteins into the
CC       mitochondrial inner membrane. Also required for the transfer of beta-
CC       barrel precursors from the TOM complex to the sorting and assembly
CC       machinery (SAM complex) of the outer membrane. Acts as a chaperone-like
CC       protein that protects the hydrophobic precursors from aggregation and
CC       guide them through the mitochondrial intermembrane space (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterohexamer; composed of 3 copies of TIM9 and 3 copies of
CC       TIM10, named soluble 70 kDa complex. Associates directly with the TIM22
CC       complex, whose core is composed of TIM22 and TIM54. Interacts with the
CC       transmembrane regions of multi-pass transmembrane proteins in transit
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000269|PubMed:10806421}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:10806421}; Intermembrane side
CC       {ECO:0000269|PubMed:10806421}.
CC   -!- DOMAIN: The twin CX3C motif contains 4 conserved Cys residues that form
CC       2 disulfide bonds in the mitochondrial intermembrane space. However,
CC       during the transit of TIM10 from cytoplasm into mitochondrion, the Cys
CC       residues probably coordinate zinc, thereby preventing folding and
CC       allowing its transfer across mitochondrial outer membrane (By
CC       similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the small Tim family. {ECO:0000305}.
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DR   EMBL; AJ243940; CAB82171.1; -; Genomic_DNA.
DR   EMBL; AJ243940; CAB82172.1; -; Genomic_DNA.
DR   EMBL; FO082053; CCE79998.1; -; Genomic_DNA.
DR   EMBL; FO082052; CCE80763.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9P335; -.
DR   SMR; Q9P335; -.
DR   STRING; 559304.Q9P335; -.
DR   EnsemblFungi; CCE79998; CCE79998; GNLVRS01_PISO0G04758g.
DR   EnsemblFungi; CCE80763; CCE80763; GNLVRS01_PISO0H04759g.
DR   eggNOG; KOG3480; Eukaryota.
DR   HOGENOM; CLU_162151_1_0_1; -.
DR   InParanoid; Q9P335; -.
DR   OMA; CIPDVND; -.
DR   Proteomes; UP000005222; Chromosome G.
DR   Proteomes; UP000005222; Chromosome H.
DR   GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0042719; C:mitochondrial intermembrane space protein transporter complex; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:InterPro.
DR   Gene3D; 1.10.287.810; -; 1.
DR   InterPro; IPR027100; Tim10.
DR   InterPro; IPR004217; Tim10-like.
DR   InterPro; IPR035427; Tim10-like_dom_sf.
DR   PANTHER; PTHR11038:SF16; PTHR11038:SF16; 1.
DR   Pfam; PF02953; zf-Tim10_DDP; 1.
DR   SUPFAM; SSF144122; SSF144122; 1.
PE   3: Inferred from homology;
KW   Chaperone; Disulfide bond; Membrane; Metal-binding; Mitochondrion;
KW   Mitochondrion inner membrane; Protein transport; Reference proteome;
KW   Translocation; Transport; Zinc.
FT   CHAIN           1..90
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim10"
FT                   /id="PRO_0000228064"
FT   MOTIF           37..62
FT                   /note="Twin CX3C motif"
FT   DISULFID        37..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        41..58
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   90 AA;  9935 MW;  755FF7302FA9C62C CRC64;
     MFGLGGAPQI SSEQKLQAAE AELDMVTGMF NQLVDQCHSK CINKSYGDSD ITKQEALCLD
     RCVAKYFDTN VQVGEHMQKL GQSGQFMGRK