ID   BSHC_STAA1              Reviewed;         537 AA.
AC   A7X1B5;
DT   16-JUN-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Putative cysteine ligase BshC {ECO:0000255|HAMAP-Rule:MF_01867};
DE            EC=6.-.-.- {ECO:0000255|HAMAP-Rule:MF_01867};
GN   Name=bshC {ECO:0000255|HAMAP-Rule:MF_01867}; OrderedLocusNames=SAHV_1167;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Involved in bacillithiol (BSH) biosynthesis. May catalyze the
CC       last step of the pathway, the addition of cysteine to glucosamine
CC       malate (GlcN-Mal) to generate BSH. {ECO:0000255|HAMAP-Rule:MF_01867}.
CC   -!- SIMILARITY: Belongs to the BshC family. {ECO:0000255|HAMAP-
CC       Rule:MF_01867}.
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DR   EMBL; AP009324; BAF78050.1; -; Genomic_DNA.
DR   RefSeq; WP_000340465.1; NC_009782.1.
DR   AlphaFoldDB; A7X1B5; -.
DR   SMR; A7X1B5; -.
DR   KEGG; saw:SAHV_1167; -.
DR   HOGENOM; CLU_022249_0_0_9; -.
DR   OMA; TTGHQLN; -.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01867; BshC; 1.
DR   InterPro; IPR011199; Bacillithiol_biosynth_BshC.
DR   Pfam; PF10079; BshC; 1.
DR   PIRSF; PIRSF012535; UCP012535; 1.
DR   TIGRFAMs; TIGR03998; thiol_BshC; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Ligase.
FT   CHAIN           1..537
FT                   /note="Putative cysteine ligase BshC"
FT                   /id="PRO_0000378258"
FT   COILED          422..450
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01867"
SQ   SEQUENCE   537 AA;  62834 MW;  85D9D08BB2B3ADCD CRC64;
     MDCKVVSLNE KDQFIPKIKS SDPVITGLFQ YDAAQQTSFE KRMSKENNGR EAALANVIRE
     YMSDLKLSNE QELNIQHLAN GSKVVIGGQQ AGLFGGPLYT FHKIFSIITL SKELTDTHKQ
     QVVPVFWIAG EDHDFDEVNH TFVYNENHGS LHKVKYHTME MPETTVSRYY PDKAELKQTL
     KTMFIHMKET VHTQGLLEIC DRIIDQYDSW TDMFKALLHE TFKAYGVLFI DAQFEPLRKM
     EAPMFKKILK KHQLLDDAFR ATQQRTQNQG LNAMIQTDTN VHLFLHDENM RQLVSYDGKH
     FKLNKTDKTY IKEEIINIAE NQPELFSNNV VTRPLMEEWL FNTVAFVGGP SEIKYWAELK
     DVFELFDVEM PIVMPRLRIT YLNDRIEKLL SKYNIPLEKV LVDGVEGERS KFIREQASHQ
     FIEKVEGMIE QQRRLNKDLL DEVAGNQNNI NLVNKNNEIH IQQYDYLLKR YLLNIEREND
     ISMKQFREIQ ETLHPMGGLQ ERIWNPLQIL NDFGTDVFKP STYPPLSYTF DHIIIKP