TIM14_CANAL
ID TIM14_CANAL Reviewed; 157 AA.
AC Q59SI2; A0A1D8PL20;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM14;
DE AltName: Full=Presequence translocated-associated motor subunit PAM18;
GN Name=PAM18; Synonyms=TIM14; OrderedLocusNames=CAALFM_C400520WA;
GN ORFNames=CaO19.11667, CaO19.4190;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Essential component of the PAM complex, a complex required
CC for the translocation of transit peptide-containing proteins from the
CC inner membrane into the mitochondrial matrix in an ATP-dependent
CC manner. In the complex, it is required to stimulate activity of mtHSP70
CC (SSC1) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with PAM16. Component of the PAM complex, at least
CC composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- DOMAIN: The J domain is essential for co-chaperone activity and
CC mediates the heterodimerization with the J-like domain of PAM16.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM14 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP017626; AOW28845.1; -; Genomic_DNA.
DR RefSeq; XP_712644.1; XM_707551.1.
DR AlphaFoldDB; Q59SI2; -.
DR SMR; Q59SI2; -.
DR STRING; 237561.Q59SI2; -.
DR GeneID; 3645747; -.
DR KEGG; cal:CAALFM_C400520WA; -.
DR CGD; CAL0000200634; PAM18.
DR VEuPathDB; FungiDB:C4_00520W_A; -.
DR HOGENOM; CLU_017633_13_0_1; -.
DR InParanoid; Q59SI2; -.
DR OMA; EGSAEWY; -.
DR OrthoDB; 1600166at2759; -.
DR PRO; PR:Q59SI2; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IBA:GO_Central.
DR GO; GO:0001671; F:ATPase activator activity; IBA:GO_Central.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR036869; J_dom_sf.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 3: Inferred from homology;
KW Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..157
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM14"
FT /id="PRO_0000071109"
FT TOPO_DOM 1..56
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 57..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..157
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT DOMAIN 99..157
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ SEQUENCE 157 AA; 17297 MW; 764D3BD9383B7366 CRC64;
MAPLEAPILA IPGENNTHQQ QQQQFQYTGQ LQKKKAAEGS IEWYFDQTIN FMGDHPVITG
IGAFAVAYFA AGFIKSNQPG INGKAFVKGG FGAKMTAKEA LQILNLKETN LSKLKLKEQH
RKLMMANHPD KGGSSYIATK INEAKDFLDK RGGMKPK
