ID   TIM14_CANGA             Reviewed;         153 AA.
AC   Q6FPU1;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM14;
DE   AltName: Full=Presequence translocated-associated motor subunit PAM18;
GN   Name=PAM18; Synonyms=TIM14; OrderedLocusNames=CAGL0J00935g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. In the complex, it is required to stimulate activity of mtHSP70
CC       (SSC1) (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with PAM16. Component of the PAM complex, at least
CC       composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The J domain is essential for co-chaperone activity and
CC       mediates the heterodimerization with the J-like domain of PAM16.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM14 family. {ECO:0000305}.
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DR   EMBL; CR380956; CAG60700.1; -; Genomic_DNA.
DR   RefSeq; XP_447753.1; XM_447753.1.
DR   AlphaFoldDB; Q6FPU1; -.
DR   SMR; Q6FPU1; -.
DR   STRING; 5478.XP_447753.1; -.
DR   EnsemblFungi; CAG60700; CAG60700; CAGL0J00935g.
DR   GeneID; 2889874; -.
DR   KEGG; cgr:CAGL0J00935g; -.
DR   CGD; CAL0133248; CAGL0J00935g.
DR   VEuPathDB; FungiDB:CAGL0J00935g; -.
DR   eggNOG; KOG0723; Eukaryota.
DR   HOGENOM; CLU_017633_13_0_1; -.
DR   InParanoid; Q6FPU1; -.
DR   OMA; EGSAEWY; -.
DR   Proteomes; UP000002428; Chromosome J.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IEA:EnsemblFungi.
DR   GO; GO:0001671; F:ATPase activator activity; IEA:EnsemblFungi.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IEA:EnsemblFungi.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   SMART; SM00271; DnaJ; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
PE   3: Inferred from homology;
KW   Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..153
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM14"
FT                   /id="PRO_0000071110"
FT   TOPO_DOM        1..43
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        44..66
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        67..153
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          96..153
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ   SEQUENCE   153 AA;  16156 MW;  5B8DF17CF04C8BFD CRC64;
     MDGTGISDGS SVTGDAAAGF PAGATQAPGS KQGMDLYFDN ALQYMGEHPV LAGVGGFLAL
     YVGAGVYKGV QTRLNGGKAA TQFLKGGFDP KMNAKEALQI LNLKENNLTT KKLKEVHRKI
     MLANHPDKGG SPYLATKINE AKDFLEKKGI VRK