TIM14_GIBZE
ID TIM14_GIBZE Reviewed; 105 AA.
AC Q4I7T5; A0A0E0SE02; V6RE08;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2005, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM14;
DE AltName: Full=Presequence translocated-associated motor subunit PAM18;
GN Name=PAM18; Synonyms=TIM14; ORFNames=FGRRES_06723, FGSG_06723;
OS Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084
OS / PH-1) (Wheat head blight fungus) (Fusarium graminearum).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium.
OX NCBI_TaxID=229533;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=17823352; DOI=10.1126/science.1143708;
RA Cuomo C.A., Gueldener U., Xu J.-R., Trail F., Turgeon B.G., Di Pietro A.,
RA Walton J.D., Ma L.-J., Baker S.E., Rep M., Adam G., Antoniw J., Baldwin T.,
RA Calvo S.E., Chang Y.-L., DeCaprio D., Gale L.R., Gnerre S., Goswami R.S.,
RA Hammond-Kosack K., Harris L.J., Hilburn K., Kennell J.C., Kroken S.,
RA Magnuson J.K., Mannhaupt G., Mauceli E.W., Mewes H.-W., Mitterbauer R.,
RA Muehlbauer G., Muensterkoetter M., Nelson D., O'Donnell K., Ouellet T.,
RA Qi W., Quesneville H., Roncero M.I.G., Seong K.-Y., Tetko I.V., Urban M.,
RA Waalwijk C., Ward T.J., Yao J., Birren B.W., Kistler H.C.;
RT "The Fusarium graminearum genome reveals a link between localized
RT polymorphism and pathogen specialization.";
RL Science 317:1400-1402(2007).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1;
RX PubMed=26198851; DOI=10.1186/s12864-015-1756-1;
RA King R., Urban M., Hammond-Kosack M.C.U., Hassani-Pak K.,
RA Hammond-Kosack K.E.;
RT "The completed genome sequence of the pathogenic ascomycete fungus Fusarium
RT graminearum.";
RL BMC Genomics 16:544-544(2015).
CC -!- FUNCTION: Essential component of the PAM complex, a complex required
CC for the translocation of transit peptide-containing proteins from the
CC inner membrane into the mitochondrial matrix in an ATP-dependent
CC manner. In the complex, it is required to stimulate activity of mtHSP70
CC (SSC1) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with PAM16. Component of the PAM complex, at least
CC composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- DOMAIN: The J domain is essential for co-chaperone activity and
CC mediates the heterodimerization with the J-like domain of PAM16.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM14 family. {ECO:0000305}.
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DR EMBL; DS231666; ESU12853.1; -; Genomic_DNA.
DR EMBL; HG970335; CEF84665.1; -; Genomic_DNA.
DR RefSeq; XP_011326360.1; XM_011328058.1.
DR AlphaFoldDB; Q4I7T5; -.
DR SMR; Q4I7T5; -.
DR STRING; 5518.FGSG_06723P0; -.
DR EnsemblFungi; ESU12853; ESU12853; FGSG_06723.
DR GeneID; 23553842; -.
DR KEGG; fgr:FGSG_06723; -.
DR VEuPathDB; FungiDB:FGRAMPH1_01G23005; -.
DR eggNOG; KOG0723; Eukaryota.
DR HOGENOM; CLU_017633_13_3_1; -.
DR InParanoid; Q4I7T5; -.
DR Proteomes; UP000070720; Chromosome 4.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR036869; J_dom_sf.
DR SUPFAM; SSF46565; SSF46565; 1.
PE 3: Inferred from homology;
KW Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..105
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM14"
FT /id="PRO_0000071113"
FT TOPO_DOM 1..3
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 4..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..105
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT DOMAIN 52..105
FT /note="J"
SQ SEQUENCE 105 AA; 11293 MW; 3B978003032CAB7E CRC64;
MASVMAWGAG AAVAAFLGRA GLVAWRRSRG GVGAMGKAFY KGGFEAKMTK KEATLILSLN
ERAITKDKVR KAHRTLMLLN HPDRGGSPYL ATKVNEAKEF LDKNG
