TIM14_SCHPO
ID TIM14_SCHPO Reviewed; 140 AA.
AC Q9UT37;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim14;
DE AltName: Full=Presequence translocated-associated motor subunit pam18;
GN Name=pam18; Synonyms=tim14; ORFNames=SPAC824.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
CC -!- FUNCTION: Essential component of the PAM complex, a complex required
CC for the translocation of transit peptide-containing proteins from the
CC inner membrane into the mitochondrial matrix in an ATP-dependent
CC manner. In the complex, it is required to stimulate activity of mtHSP70
CC (ssc1) (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with pam16. Component of the PAM complex, at least
CC composed of mtHsp70, mge1, tim44, pam16, pam17 and pam18 (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}.
CC -!- DOMAIN: The J domain is essential for co-chaperone activity and
CC mediates the heterodimerization with the J-like domain of PAM16.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM14 family. {ECO:0000305}.
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DR EMBL; CU329670; CAB57336.1; -; Genomic_DNA.
DR PIR; T39107; T39107.
DR RefSeq; NP_593445.1; NM_001018878.2.
DR AlphaFoldDB; Q9UT37; -.
DR SMR; Q9UT37; -.
DR BioGRID; 279229; 1.
DR STRING; 4896.SPAC824.06.1; -.
DR MaxQB; Q9UT37; -.
DR PaxDb; Q9UT37; -.
DR EnsemblFungi; SPAC824.06.1; SPAC824.06.1:pep; SPAC824.06.
DR GeneID; 2542780; -.
DR KEGG; spo:SPAC824.06; -.
DR PomBase; SPAC824.06; -.
DR VEuPathDB; FungiDB:SPAC824.06; -.
DR eggNOG; KOG0723; Eukaryota.
DR HOGENOM; CLU_017633_13_3_1; -.
DR InParanoid; Q9UT37; -.
DR OMA; MTKWEAA; -.
DR PhylomeDB; Q9UT37; -.
DR PRO; PR:Q9UT37; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:PomBase.
DR GO; GO:0001405; C:PAM complex, Tim23 associated import motor; ISO:PomBase.
DR GO; GO:0001671; F:ATPase activator activity; ISO:PomBase.
DR GO; GO:0030544; F:Hsp70 protein binding; ISM:PomBase.
DR GO; GO:0051082; F:unfolded protein binding; ISO:PomBase.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; ISO:PomBase.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR036869; J_dom_sf.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 3: Inferred from homology;
KW Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..140
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim14"
FT /id="PRO_0000071116"
FT TOPO_DOM 1..6
FT /note="Mitochondrial intermembrane"
FT /evidence="ECO:0000255"
FT TRANSMEM 7..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..140
FT /note="Mitochondrial matrix"
FT /evidence="ECO:0000255"
FT DOMAIN 51..113
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
SQ SEQUENCE 140 AA; 15271 MW; EA5EF4F6684B3ADA CRC64;
MSSAILLGVG IAATAAAGKI GVDAFRKYRN LNGGVKAFLK GGFESKMSRA EAIQILSLNN
RTLTRQKIKE AHRRLMLANH PDRGGSPYVA SKVNEAKSLL DADRSIRKFS SWALPVSKQR
SMPSVLEAVK WLEYSSIPKA
