ID   TIM14_YARLI             Reviewed;         148 AA.
AC   Q6CEU0;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM14;
DE   AltName: Full=Presequence translocated-associated motor subunit PAM18;
GN   Name=PAM18; Synonyms=TIM14; OrderedLocusNames=YALI0B12958g;
OS   Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Dipodascaceae; Yarrowia.
OX   NCBI_TaxID=284591;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CLIB 122 / E 150;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. In the complex, it is required to stimulate activity of mtHSP70
CC       (SSC1) (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with PAM16. Component of the PAM complex, at least
CC       composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The J domain is essential for co-chaperone activity and
CC       mediates the heterodimerization with the J-like domain of PAM16.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM14 family. {ECO:0000305}.
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DR   EMBL; CR382128; CAG83073.1; -; Genomic_DNA.
DR   RefSeq; XP_500822.1; XM_500822.1.
DR   AlphaFoldDB; Q6CEU0; -.
DR   SMR; Q6CEU0; -.
DR   STRING; 4952.CAG83073; -.
DR   EnsemblFungi; CAG83073; CAG83073; YALI0_B12958g.
DR   GeneID; 2906859; -.
DR   KEGG; yli:YALI0B12958g; -.
DR   VEuPathDB; FungiDB:YALI0_B12958g; -.
DR   HOGENOM; CLU_017633_13_0_1; -.
DR   InParanoid; Q6CEU0; -.
DR   OMA; KMGPSEA; -.
DR   Proteomes; UP000001300; Chromosome B.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IBA:GO_Central.
DR   GO; GO:0001671; F:ATPase activator activity; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   SUPFAM; SSF46565; SSF46565; 1.
PE   3: Inferred from homology;
KW   Chaperone; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..148
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM14"
FT                   /id="PRO_0000071117"
FT   TOPO_DOM        1..50
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..68
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        69..148
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          92..148
FT                   /note="J"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   148 AA;  15927 MW;  0A7B32C022B94A2E CRC64;
     MSTTPVQPLQ SEPLMDSETG VAPQIEAPQV PEGPKKGIDE QIFDYFAEHP VQATAATLVG
     LYALGAVFKR PAAGARGQFF KGGFENKMGP SEALQILSLR DAGLTMNKLK GQHRKIMLLN
     HPDRGGSPYV ATKINEAKSV LEKRGGLK