ID   TIM16_ASHGO             Reviewed;         136 AA.
AC   Q754J4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   25-MAY-2022, entry version 95.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM16;
DE   AltName: Full=Presequence translocated-associated motor subunit PAM16;
GN   Name=PAM16; Synonyms=TIM16; OrderedLocusNames=AFR078W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION, AND SEQUENCE REVISION TO 76 AND 78.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. In the complex, it is required to regulate activity of mtHSP70
CC       (SSC1) via its interaction with PAM18/TIM14. May act by positioning
CC       PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize
CC       ATPase stimulation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with PAM18. Component of the PAM complex, at least
CC       composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The J-like region, although related to the J domain does not
CC       stimulate ATPase activity of mtHSP70. It nevertheless mediates the
CC       heterodimerization with the J domain of PAM18 and is therefore
CC       essential for PAM complex function (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
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DR   EMBL; AE016819; AAS53449.2; -; Genomic_DNA.
DR   RefSeq; NP_985625.2; NM_210979.2.
DR   AlphaFoldDB; Q754J4; -.
DR   SMR; Q754J4; -.
DR   STRING; 33169.AAS53449; -.
DR   EnsemblFungi; AAS53449; AAS53449; AGOS_AFR078W.
DR   GeneID; 4621867; -.
DR   KEGG; ago:AGOS_AFR078W; -.
DR   eggNOG; KOG3442; Eukaryota.
DR   HOGENOM; CLU_101461_0_1_1; -.
DR   InParanoid; Q754J4; -.
DR   OMA; RMFKIND; -.
DR   Proteomes; UP000000591; Chromosome VI.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IEA:EnsemblFungi.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:EnsemblFungi.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR005341; Tim16.
DR   PANTHER; PTHR12388; PTHR12388; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transport.
FT   CHAIN           1..136
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM16"
FT                   /id="PRO_0000214088"
FT   REGION          58..114
FT                   /note="J-like"
FT   REGION          114..136
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   136 AA;  15233 MW;  316677ABAF868212 CRC64;
     MAHRVLVQVI FTGARVFGRA FTEAYKQTAA QMAKQGTSSA ARSQGGMTNE YGGITLDESC
     KILNIEENGP EMNLDKVEQR FKYLFDINDK EKGGSFYLQS KIYRAAERLK WELAQREQAD
     KGASAHPQDQ QPGPQN