ID   TIM16_DICDI             Reviewed;         113 AA.
AC   Q54SV6;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim16;
DE   AltName: Full=Presequence translocated-associated motor subunit pam16;
GN   Name=timm16; Synonyms=pam16, tim16; ORFNames=DDB_G0282195;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Regulates ATP-dependent protein translocation into the
CC       mitochondrial matrix. {ECO:0000250}.
CC   -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC       mitochondrial HSP70 protein, grepE, tim16 and tim14. Associates with
CC       the TIM23 complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}; Matrix side {ECO:0000250}.
CC   -!- DOMAIN: The J-like region, although related to the J domain does not
CC       have co-chaperone activity. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
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DR   EMBL; AAFI02000046; EAL66303.1; -; Genomic_DNA.
DR   RefSeq; XP_640279.1; XM_635187.1.
DR   AlphaFoldDB; Q54SV6; -.
DR   SMR; Q54SV6; -.
DR   STRING; 44689.DDB0238039; -.
DR   PaxDb; Q54SV6; -.
DR   EnsemblProtists; EAL66303; EAL66303; DDB_G0282195.
DR   GeneID; 8623453; -.
DR   KEGG; ddi:DDB_G0282195; -.
DR   dictyBase; DDB_G0282195; timm16.
DR   eggNOG; ENOG502R0BQ; Eukaryota.
DR   HOGENOM; CLU_101461_2_1_1; -.
DR   InParanoid; Q54SV6; -.
DR   OMA; AKYLIQI; -.
DR   PhylomeDB; Q54SV6; -.
DR   PRO; PR:Q54SV6; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; ISS:dictyBase.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; ISS:dictyBase.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR005341; Tim16.
DR   PANTHER; PTHR12388; PTHR12388; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transport.
FT   CHAIN           1..113
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim16"
FT                   /id="PRO_0000331205"
FT   REGION          56..108
FT                   /note="J-like"
SQ   SEQUENCE   113 AA;  12221 MW;  88ED00CC90DA1AA2 CRC64;
     MAARLIAKIV FTSGTVLVRS IQMAYKQALL QAESGMGAAA GSMDVKSKMS PIEARKILGL
     ENVETVSKED IDKKYNELLT INDPKDGGSE YLQIKISGAK HCLHSALKEG KKI