TIM16_DROME
ID TIM16_DROME Reviewed; 141 AA.
AC Q9VF08; Q9GP65;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit Tim16;
DE AltName: Full=Protein black pearl;
GN Name=blp; ORFNames=CG5268;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC TISSUE=Embryo;
RX PubMed=11179663; DOI=10.1016/s0378-1119(00)00548-5;
RA Becker S., Gehrsitz A., Bork P., Buchner S., Buchner E.;
RT "The black-pearl gene of Drosophila defines a novel conserved protein
RT family and is required for early larval survival.";
RL Gene 262:15-22(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Regulates ATP-dependent protein translocation into the
CC mitochondrial matrix (By similarity). Essential for larval development.
CC {ECO:0000250, ECO:0000269|PubMed:11179663}.
CC -!- SUBUNIT: Probable component of the PAM complex at least composed of a
CC mitochondrial HSP70 protein, Roe1, TIM44, blp/TIM16 and TIM14.
CC Associates with the TIM23 complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC Peripheral membrane protein {ECO:0000250}; Matrix side {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in distinct cells in the embryonic and
CC larval nervous system. {ECO:0000269|PubMed:11179663}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in early embryos.
CC {ECO:0000269|PubMed:11179663}.
CC -!- DOMAIN: The J-like region, although related to the J domain does not
CC have co-chaperone activity. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
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DR EMBL; AJ278732; CAC20095.1; -; mRNA.
DR EMBL; AE014297; AAF55254.1; -; Genomic_DNA.
DR EMBL; AY071034; AAL48656.1; -; mRNA.
DR RefSeq; NP_001287349.1; NM_001300420.1.
DR RefSeq; NP_524370.2; NM_079646.4.
DR AlphaFoldDB; Q9VF08; -.
DR SMR; Q9VF08; -.
DR BioGRID; 66991; 1.
DR IntAct; Q9VF08; 7.
DR STRING; 7227.FBpp0082657; -.
DR PaxDb; Q9VF08; -.
DR PRIDE; Q9VF08; -.
DR DNASU; 41937; -.
DR EnsemblMetazoa; FBtr0083203; FBpp0082657; FBgn0038387.
DR EnsemblMetazoa; FBtr0345214; FBpp0311409; FBgn0038387.
DR GeneID; 41937; -.
DR KEGG; dme:Dmel_CG5268; -.
DR CTD; 41937; -.
DR FlyBase; FBgn0038387; blp.
DR VEuPathDB; VectorBase:FBgn0038387; -.
DR eggNOG; KOG3442; Eukaryota.
DR GeneTree; ENSGT00390000012037; -.
DR HOGENOM; CLU_101461_3_0_1; -.
DR InParanoid; Q9VF08; -.
DR OMA; AKYLIQI; -.
DR OrthoDB; 1640138at2759; -.
DR PhylomeDB; Q9VF08; -.
DR BioGRID-ORCS; 41937; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Baldspot; fly.
DR GenomeRNAi; 41937; -.
DR PRO; PR:Q9VF08; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0038387; Expressed in Malpighian tubule and 18 other tissues.
DR ExpressionAtlas; Q9VF08; baseline and differential.
DR Genevisible; Q9VF08; DM.
DR GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR GO; GO:0002168; P:instar larval development; IMP:FlyBase.
DR GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR036869; J_dom_sf.
DR InterPro; IPR005341; Tim16.
DR PANTHER; PTHR12388; PTHR12388; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Protein transport; Reference proteome;
KW Translocation; Transport.
FT CHAIN 1..141
FT /note="Mitochondrial import inner membrane translocase
FT subunit Tim16"
FT /id="PRO_0000214087"
FT REGION 34..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 60..113
FT /note="J-like"
FT REGION 108..141
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 35
FT /note="A -> V (in Ref. 1; CAC20095)"
FT /evidence="ECO:0000305"
FT CONFLICT 85..87
FT /note="QVN -> HVI (in Ref. 1; CAC20095)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 141 AA; 15731 MW; 92981D4E1E1DB204 CRC64;
MAKYIAQIIV LGAQAVGRAF TKALRQEIAA SQEAARRAGG GKQGDKSAES NLRTGMTLEE
AKQILNIDDP KNVDAITKNY EHLFQVNERS KGGSFYIQSK VFRAKERLDH EIKAHEQPRS
SNTEAAQDTA EESQSRSRQR R
