ID   TIM16_KLULA             Reviewed;         139 AA.
AC   Q6CK35;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM16;
DE   AltName: Full=Presequence translocated-associated motor subunit PAM16;
GN   Name=PAM16; Synonyms=TIM16; OrderedLocusNames=KLLA0F13860g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. In the complex, it is required to regulate activity of mtHSP70
CC       (SSC1) via its interaction with PAM18/TIM14. May act by positioning
CC       PAM18/TIM14 in juxtaposition to mtHSP70 at the translocon to maximize
CC       ATPase stimulation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with PAM18. Component of the PAM complex, at least
CC       composed of mtHsp70, MGE1, TIM44, PAM16, PAM17 and PAM18 (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The J-like region, although related to the J domain does not
CC       stimulate ATPase activity of mtHSP70. It nevertheless mediates the
CC       heterodimerization with the J domain of PAM18 and is therefore
CC       essential for PAM complex function (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98412.1; -; Genomic_DNA.
DR   RefSeq; XP_455704.1; XM_455704.1.
DR   AlphaFoldDB; Q6CK35; -.
DR   SMR; Q6CK35; -.
DR   STRING; 28985.XP_455704.1; -.
DR   EnsemblFungi; CAG98412; CAG98412; KLLA0_F13860g.
DR   GeneID; 2894877; -.
DR   KEGG; kla:KLLA0_F13860g; -.
DR   eggNOG; KOG3442; Eukaryota.
DR   HOGENOM; CLU_101461_0_1_1; -.
DR   InParanoid; Q6CK35; -.
DR   OMA; RMFKIND; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IEA:EnsemblFungi.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:EnsemblFungi.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IEA:EnsemblFungi.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR005341; Tim16.
DR   PANTHER; PTHR12388; PTHR12388; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transport.
FT   CHAIN           1..139
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM16"
FT                   /id="PRO_0000214094"
FT   REGION          56..111
FT                   /note="J-like"
FT   REGION          115..139
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   139 AA;  15622 MW;  29E9724B5270DE99 CRC64;
     MAHRAFIQVI FTGAQVFGRA FAESYRQAAA QTAKQTANAS RGRGASAEYG GITLDESSKI
     LNIENEQDMN LDKINERFKY LFEINDKEKG GSFYLQSKIY RAAERLKYEL AEKEKAANPE
     LNQEQAKSSS TTESPELKK