ID   TIM16_NEUCR             Reviewed;         141 AA.
AC   Q7S6S4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim16;
DE   AltName: Full=Presequence translocated-associated motor subunit pam16;
GN   Name=un-4; Synonyms=pam16, tim16; ORFNames=NCU05515;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Essential component of the PAM complex, a complex required
CC       for the translocation of transit peptide-containing proteins from the
CC       inner membrane into the mitochondrial matrix in an ATP-dependent
CC       manner. In the complex, it is required to regulate activity of mtHSP70
CC       (hsp70-5) via its interaction with tim14/pam18. May act by positioning
CC       tim14/pam18 in juxtaposition to mtHSP70 at the translocon to maximize
CC       ATPase stimulation (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Heterodimer with tim14/pam18. Component of the PAM complex, at
CC       least composed of hsp70-5/ssc1, grpe/mge1, tim44, un-4/pam16, pam17 and
CC       tim14/pam18 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Peripheral membrane protein {ECO:0000250}.
CC   -!- DOMAIN: The J-like region, although related to the J domain does not
CC       stimulate ATPase activity of mtHSP70. It nevertheless mediates the
CC       heterodimerization with the J domain of PAM18 and is therefore
CC       essential for PAM complex function (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM16/PAM16 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CM002241; EAA31241.1; -; Genomic_DNA.
DR   RefSeq; XP_960477.1; XM_955384.2.
DR   AlphaFoldDB; Q7S6S4; -.
DR   SMR; Q7S6S4; -.
DR   STRING; 5141.EFNCRP00000005530; -.
DR   EnsemblFungi; EAA31241; EAA31241; NCU05515.
DR   GeneID; 3876599; -.
DR   KEGG; ncr:NCU05515; -.
DR   VEuPathDB; FungiDB:NCU05515; -.
DR   HOGENOM; CLU_101461_0_1_1; -.
DR   InParanoid; Q7S6S4; -.
DR   OMA; AKYLIQI; -.
DR   Proteomes; UP000001805; Chromosome 5, Linkage Group VI.
DR   GO; GO:0001405; C:PAM complex, Tim23 associated import motor; IEA:EnsemblFungi.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0019904; F:protein domain specific binding; IEA:EnsemblFungi.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 1.10.287.110; -; 1.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR005341; Tim16.
DR   PANTHER; PTHR12388; PTHR12388; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transport.
FT   CHAIN           1..141
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim16"
FT                   /id="PRO_0000214095"
FT   REGION          59..117
FT                   /note="J-like"
FT   REGION          119..141
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   141 AA;  15805 MW;  8FDF293024EC167E CRC64;
     MAYRLITQVV VVGSRVLGRA FAEAYKQAAA SSQYQRAQQK NGNAATGRAS LTSGMTLDEA
     CKILNVNKPA DGTAANMEEV MERFKRLFDA NDPEKGGSFY LQSKVVRARE RLEAEIKPKM
     EEKQAEEEVK EGWNPKIYKD R