ID   TIM21_ASHGO             Reviewed;         234 AA.
AC   Q75CX4;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM21;
DE   Flags: Precursor;
GN   Name=TIM21; OrderedLocusNames=ACL183W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to keep the TOM and
CC       the TIM23 complexes in close contact. At some point, it is released
CC       from the TOM23 complex to allow protein translocation into the
CC       mitochondrial matrix (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of TIM23,
CC       TIM17, TIM50 and TIM21. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM21 family. {ECO:0000305}.
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DR   EMBL; AE016816; AAS51045.1; -; Genomic_DNA.
DR   RefSeq; NP_983221.1; NM_208574.1.
DR   AlphaFoldDB; Q75CX4; -.
DR   SMR; Q75CX4; -.
DR   STRING; 33169.AAS51045; -.
DR   PRIDE; Q75CX4; -.
DR   EnsemblFungi; AAS51045; AAS51045; AGOS_ACL183W.
DR   GeneID; 4619317; -.
DR   KEGG; ago:AGOS_ACL183W; -.
DR   eggNOG; KOG4836; Eukaryota.
DR   HOGENOM; CLU_089043_1_0_1; -.
DR   InParanoid; Q75CX4; -.
DR   OMA; YEWGELS; -.
DR   Proteomes; UP000000591; Chromosome III.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.10.450.320; -; 1.
DR   InterPro; IPR013261; Tim21.
DR   InterPro; IPR038552; Tim21_IMS_sf.
DR   PANTHER; PTHR13032; PTHR13032; 1.
DR   Pfam; PF08294; TIM21; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..234
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM21"
FT                   /id="PRO_0000043140"
FT   TOPO_DOM        ?..71
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        93..234
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   234 AA;  25929 MW;  2E899BB344320AE0 CRC64;
     MLAFGSICGR LRAPAGAGLR VNVGKMPGRA WQVANGQPYS TFYAPPEQAG NRQKERRVTA
     WKKVRAAATF SASGMLVLGA AGVAGIVLYL ILSELFSPSG DTQIFNRAVS TVEGDAVARS
     LLQCEDGVHR SERLKAYGDS VGDDRWTRNR PISSTRRLDA SGREHYYMRF HVETGRRRGV
     VSLEAQQSDD SYQPEFVRMY LDVPGEKRHY LIRPEPSVAK PKGFLGLNWG PRKD