ID   TIM21_KLULA             Reviewed;         234 AA.
AC   Q6CW96;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM21;
DE   Flags: Precursor;
GN   Name=TIM21; OrderedLocusNames=KLLA0B05786g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to keep the TOM and
CC       the TIM23 complexes in close contact. At some point, it is released
CC       from the TOM23 complex to allow protein translocation into the
CC       mitochondrial matrix (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of TIM23,
CC       TIM17, TIM50 and TIM21. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM21 family. {ECO:0000305}.
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DR   EMBL; CR382122; CAH02186.1; -; Genomic_DNA.
DR   RefSeq; XP_451793.1; XM_451793.1.
DR   AlphaFoldDB; Q6CW96; -.
DR   SMR; Q6CW96; -.
DR   STRING; 28985.XP_451793.1; -.
DR   EnsemblFungi; CAH02186; CAH02186; KLLA0_B05786g.
DR   GeneID; 2897309; -.
DR   KEGG; kla:KLLA0_B05786g; -.
DR   eggNOG; KOG4836; Eukaryota.
DR   HOGENOM; CLU_089043_1_0_1; -.
DR   InParanoid; Q6CW96; -.
DR   OMA; YEWGELS; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IEA:EnsemblFungi.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IEA:EnsemblFungi.
DR   Gene3D; 3.10.450.320; -; 1.
DR   InterPro; IPR013261; Tim21.
DR   InterPro; IPR038552; Tim21_IMS_sf.
DR   PANTHER; PTHR13032; PTHR13032; 1.
DR   Pfam; PF08294; TIM21; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..234
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM21"
FT                   /id="PRO_0000043146"
FT   TOPO_DOM        ?..71
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        93..234
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   234 AA;  26566 MW;  F5CD70806DBB6228 CRC64;
     MFCQFISRPA QSLSGKFSSK TLCLPLIASP VRIKHTVQGY STFNAQQNAG NKQQKKKIPL
     WPRIRAFTTF TFSGILVIGA TGLAGVVIYL IGAELFSPSG DTQIFNRAVS KVENDEVARR
     LLKCNDTETS TERLKAYGEI LTDDRWTRNR PISSTRRIDK EGKEHYLMRF HVESKQKMGI
     VHVEAKESDV NYQPDFVSMY LDIPGEKRHY LIKPKLSIVK PKGFLGVNWG PKRD