ID   TIM21_NEUCR             Reviewed;         251 AA.
AC   Q7S8S5;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   25-MAY-2022, entry version 91.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim21;
DE   Flags: Precursor;
GN   Name=tim21; ORFNames=NCU08810;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Essential component of the TIM23 complex, a complex that
CC       mediates the translocation of transit peptide-containing proteins
CC       across the mitochondrial inner membrane. Required to keep the TOM and
CC       the TIM23 complexes in close contact. At some point, it is released
CC       from the TOM23 complex to allow protein translocation into the
CC       mitochondrial matrix (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM23 complex, at least composed of tim23,
CC       tim17, tim50 and tim21. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Single-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TIM21 family. {ECO:0000305}.
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DR   EMBL; CM002239; EAA32761.1; -; Genomic_DNA.
DR   RefSeq; XP_961997.1; XM_956904.3.
DR   AlphaFoldDB; Q7S8S5; -.
DR   SMR; Q7S8S5; -.
DR   STRING; 5141.EFNCRP00000008735; -.
DR   EnsemblFungi; EAA32761; EAA32761; NCU08810.
DR   GeneID; 3878145; -.
DR   KEGG; ncr:NCU08810; -.
DR   VEuPathDB; FungiDB:NCU08810; -.
DR   HOGENOM; CLU_089407_0_0_1; -.
DR   InParanoid; Q7S8S5; -.
DR   OMA; FRYVFVE; -.
DR   Proteomes; UP000001805; Chromosome 4, Linkage Group IV.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; IBA:GO_Central.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; IBA:GO_Central.
DR   Gene3D; 3.10.450.320; -; 1.
DR   InterPro; IPR013261; Tim21.
DR   InterPro; IPR038552; Tim21_IMS_sf.
DR   PANTHER; PTHR13032; PTHR13032; 1.
DR   Pfam; PF08294; TIM21; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Transit peptide; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   TRANSIT         1..51
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           52..251
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim21"
FT                   /id="PRO_0000043147"
FT   TOPO_DOM        52..90
FT                   /note="Mitochondrial matrix"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        91..111
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        112..251
FT                   /note="Mitochondrial intermembrane"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   251 AA;  27798 MW;  A65EFE39AD40B923 CRC64;
     MMKMLTGTSA GVRLLLSPAV VRQQATITTA ASRALPPTAA SVILVSSRRQ YATTQESSKR
     RSVTPFNDDG HVPWTRLSTG EKAGRAVQQT FNFGLVILGV VLTGGIAYLL FTDVFSPESK
     TAYFNRAVDR IRADPRCVAL LSPGDPKKIA AHGEETHNKW RRARPIAATV EKDNRGVEHL
     KMHFHVEGPR GSGVVGLHLT KQPGHWEHEY QTFYVDVRGH QRIYLENKEA EVAAAKKGGN
     KEFKFLGVKW N