ID   TIM21_RAT               Reviewed;         245 AA.
AC   Q5U2X7;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim21;
DE   AltName: Full=TIM21-like protein, mitochondrial;
DE   Flags: Precursor;
GN   Name=Timm21; Synonyms=Tim21;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Participates in the translocation of transit peptide-
CC       containing proteins across the mitochondrial inner membrane. Also
CC       required for assembly of mitochondrial respiratory chain complex I and
CC       complex IV as component of the MITRAC (mitochondrial translation
CC       regulation assembly intermediate of cytochrome c oxidase complex)
CC       complex. Probably shuttles between the presequence translocase and
CC       respiratory-chain assembly intermediates in a process that promotes
CC       incorporation of early nuclear-encoded subunits into these complexes.
CC       {ECO:0000250|UniProtKB:Q9BVV7}.
CC   -!- SUBUNIT: Component of the TIM23 complex. Component of the MITRAC
CC       (mitochondrial translation regulation assembly intermediate of
CC       cytochrome c oxidase complex) complex, the core components of this
CC       complex being COA3/MITRAC12 and COX14. Interacts with COA3 AND MT-
CC       CO1/COX1. {ECO:0000250|UniProtKB:Q9BVV7}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the TIM21 family. {ECO:0000305}.
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DR   EMBL; BC085823; AAH85823.1; -; mRNA.
DR   RefSeq; NP_001008344.1; NM_001008343.1.
DR   AlphaFoldDB; Q5U2X7; -.
DR   SMR; Q5U2X7; -.
DR   STRING; 10116.ENSRNOP00000020376; -.
DR   iPTMnet; Q5U2X7; -.
DR   PhosphoSitePlus; Q5U2X7; -.
DR   jPOST; Q5U2X7; -.
DR   PaxDb; Q5U2X7; -.
DR   PRIDE; Q5U2X7; -.
DR   Ensembl; ENSRNOT00000020376; ENSRNOP00000020376; ENSRNOG00000015142.
DR   GeneID; 307210; -.
DR   KEGG; rno:307210; -.
DR   CTD; 29090; -.
DR   RGD; 1307279; Timm21.
DR   eggNOG; KOG4836; Eukaryota.
DR   GeneTree; ENSGT00390000011552; -.
DR   HOGENOM; CLU_099476_0_0_1; -.
DR   InParanoid; Q5U2X7; -.
DR   OMA; FRYVFVE; -.
DR   OrthoDB; 1232375at2759; -.
DR   PhylomeDB; Q5U2X7; -.
DR   TreeFam; TF315067; -.
DR   PRO; PR:Q5U2X7; -.
DR   Proteomes; UP000002494; Chromosome 18.
DR   Bgee; ENSRNOG00000015142; Expressed in heart and 20 other tissues.
DR   Genevisible; Q5U2X7; RN.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005744; C:TIM23 mitochondrial import inner membrane translocase complex; ISS:UniProtKB.
DR   GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; ISS:UniProtKB.
DR   GO; GO:0032981; P:mitochondrial respiratory chain complex I assembly; ISS:UniProtKB.
DR   GO; GO:0030150; P:protein import into mitochondrial matrix; ISS:UniProtKB.
DR   Gene3D; 3.10.450.320; -; 1.
DR   InterPro; IPR013261; Tim21.
DR   InterPro; IPR038552; Tim21_IMS_sf.
DR   PANTHER; PTHR13032; PTHR13032; 1.
DR   Pfam; PF08294; TIM21; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Mitochondrion; Protein transport; Reference proteome;
KW   Transit peptide; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   TRANSIT         1..18
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..245
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim21"
FT                   /id="PRO_0000043231"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          64..97
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        69..84
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   245 AA;  27876 MW;  10DCA7B2071B81ED CRC64;
     MICAFLRVVR HAEKLHGSLG RQLLLPHFVL TKACLKTQPL RWGLREQKKT VQPRTVLGFT
     QKTFWTQGPD PRKAKEDSSK QVSINRNQRE ETGVSTSQKV KEAGRDVTYL IVVLFGVSIT
     GSLLYTIFKE LFSSSSPNII YGKALGKCRT HPEVISVFGE PVKGYGEMSR RGRRQHVSFT
     EYANNGLKRI RVKFYIEGSE PGKQGTVHAE VEENPRSGQF DFRYIFVDVA PKRSIVVEDN
     RFQQS