ID   TIM22_ASHGO             Reviewed;         201 AA.
AC   Q75E80;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN   Name=TIM22; OrderedLocusNames=ABL148C;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; AE016815; AAS50623.1; -; Genomic_DNA.
DR   RefSeq; NP_982799.1; NM_208152.1.
DR   AlphaFoldDB; Q75E80; -.
DR   SMR; Q75E80; -.
DR   STRING; 33169.AAS50623; -.
DR   EnsemblFungi; AAS50623; AAS50623; AGOS_ABL148C.
DR   GeneID; 4618879; -.
DR   KEGG; ago:AGOS_ABL148C; -.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_091077_1_0_1; -.
DR   InParanoid; Q75E80; -.
DR   OMA; THSYAKN; -.
DR   Proteomes; UP000000591; Chromosome II.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..201
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM22"
FT                   /id="PRO_0000228086"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        146..162
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        167..187
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..135
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT   DISULFID        154..173
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ   SEQUENCE   201 AA;  21099 MW;  E5257C12FA240545 CRC64;
     MVYRGFGLEH ISPPVNKPFA EMTPEEQGER GAQMMMEFMT SCPGKSAISG VTGFALGGVF
     GLFMASMAYD TPLHTPAPVG AGPGAGIPGA PTLQQMADLP LKQQIKIQFA DMGRRAYSSA
     KNFGYIGMIY SGVECTIESL RAKNDLYNGV AAGCLTGGGL AYKSGPSAAL IGCAGFAAFS
     TAIDLYMRSE NGRPPKNDFD E