TIM22_ASPOR
ID TIM22_ASPOR Reviewed; 184 AA.
AC Q2UAP8;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 25-MAY-2022, entry version 74.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim22;
GN Name=tim22; ORFNames=AO090102000295;
OS Aspergillus oryzae (strain ATCC 42149 / RIB 40) (Yellow koji mold).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=510516;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 42149 / RIB 40;
RX PubMed=16372010; DOI=10.1038/nature04300;
RA Machida M., Asai K., Sano M., Tanaka T., Kumagai T., Terai G., Kusumoto K.,
RA Arima T., Akita O., Kashiwagi Y., Abe K., Gomi K., Horiuchi H.,
RA Kitamoto K., Kobayashi T., Takeuchi M., Denning D.W., Galagan J.E.,
RA Nierman W.C., Yu J., Archer D.B., Bennett J.W., Bhatnagar D.,
RA Cleveland T.E., Fedorova N.D., Gotoh O., Horikawa H., Hosoyama A.,
RA Ichinomiya M., Igarashi R., Iwashita K., Juvvadi P.R., Kato M., Kato Y.,
RA Kin T., Kokubun A., Maeda H., Maeyama N., Maruyama J., Nagasaki H.,
RA Nakajima T., Oda K., Okada K., Paulsen I., Sakamoto K., Sawano T.,
RA Takahashi M., Takase K., Terabayashi Y., Wortman J.R., Yamada O.,
RA Yamagata Y., Anazawa H., Hata Y., Koide Y., Komori T., Koyama Y.,
RA Minetoki T., Suharnan S., Tanaka A., Isono K., Kuhara S., Ogasawara N.,
RA Kikuchi H.;
RT "Genome sequencing and analysis of Aspergillus oryzae.";
RL Nature 438:1157-1161(2005).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. In the TIM22 complex, it
CC constitutes the voltage-activated and signal-gated channel. Forms a
CC twin-pore translocase that uses the membrane potential as external
CC driving force in 2 voltage-dependent steps (By similarity).
CC {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; AP007162; BAE61367.1; -; Genomic_DNA.
DR RefSeq; XP_001822500.1; XM_001822448.2.
DR AlphaFoldDB; Q2UAP8; -.
DR SMR; Q2UAP8; -.
DR STRING; 510516.Q2UAP8; -.
DR EnsemblFungi; BAE61367; BAE61367; AO090102000295.
DR GeneID; 5994545; -.
DR KEGG; aor:AO090102000295; -.
DR VEuPathDB; FungiDB:AO090102000295; -.
DR HOGENOM; CLU_091077_1_0_1; -.
DR OMA; THSYAKN; -.
DR Proteomes; UP000006564; Chromosome 4.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:EnsemblFungi.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IEA:EnsemblFungi.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IEA:EnsemblFungi.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:EnsemblFungi.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..184
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim22"
FT /id="PRO_0000228087"
FT TRANSMEM 8..28
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DISULFID 49..126
FT /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT DISULFID 145..164
FT /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ SEQUENCE 184 AA; 18708 MW; 30CEF59385C45D44 CRC64;
MNIPGMTTGM APAGATAGAG FPGAGAGMQG MSEQEQAMVK AMHAAMESCP VKTVISGTMG
FGLGGVFGLF MASMSYDSTF TPQGKAIMDL PWREQVRRGF KDMGSRSWSS AKNFGIVGAL
YSGTECCVEG LRAKNDLSNS VISGCITGGI LGAKAGPQAA AAGCAGFAAF SAAIDAYMRM
PSEE