ID   TIM22_BOVIN             Reviewed;         194 AA.
AC   Q5BIN4; A6QLX1;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   12-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim22;
GN   Name=TIMM22; Synonyms=TIM22;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal cerebellum;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIMM22, associated with peripheral protein FXC1/TIMM10B and the 70 kDa
CC       heterohexamer. In most cases, the 70 kDa complex is composed of TIMM9
CC       and TIMM10 (TIMM10A or TIMM10B). A small fraction of the 70 kDa complex
CC       is composed of TIMM8 (TIMM8A/DDP1 or TIMM8B/DDP2) and TIMM13. The TIM22
CC       complex also contains AGK and TIMM29. Interacts directly with TIMM9,
CC       TIMM10A and FXC1/TIMM10B. Interacts (when oxidized) with TIMM29;
CC       interaction is direct. {ECO:0000250|UniProtKB:Q9Y584}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y584}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- PTM: Disulfide bonds promote efficient assembly of the TIM22 complex.
CC       {ECO:0000250|UniProtKB:Q9Y584}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; BT021190; AAX31372.1; -; mRNA.
DR   EMBL; BC148116; AAI48117.1; -; mRNA.
DR   RefSeq; NP_001029670.1; NM_001034498.1.
DR   AlphaFoldDB; Q5BIN4; -.
DR   SMR; Q5BIN4; -.
DR   STRING; 9913.ENSBTAP00000011080; -.
DR   PaxDb; Q5BIN4; -.
DR   Ensembl; ENSBTAT00000011080; ENSBTAP00000011080; ENSBTAG00000008423.
DR   GeneID; 515555; -.
DR   KEGG; bta:515555; -.
DR   CTD; 29928; -.
DR   VEuPathDB; HostDB:ENSBTAG00000008423; -.
DR   VGNC; VGNC:49575; TIMM22.
DR   eggNOG; KOG3225; Eukaryota.
DR   GeneTree; ENSGT00390000016067; -.
DR   HOGENOM; CLU_091077_0_0_1; -.
DR   InParanoid; Q5BIN4; -.
DR   OMA; THSYAKN; -.
DR   OrthoDB; 1544601at2759; -.
DR   TreeFam; TF105836; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000008423; Expressed in oocyte and 106 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:UniProtKB.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..194
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim22"
FT                   /id="PRO_0000210297"
FT   TRANSMEM        74..94
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        123..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        170..190
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        69..141
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
FT   DISULFID        160..179
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
SQ   SEQUENCE   194 AA;  20156 MW;  7F18565E38396D75 CRC64;
     MAATAPKAGG SAPEAAASAE APLQYSLLLQ YLVGDKRQPR LLEPGSLGGI PSPAKSEEQK
     MIERAMESCA FKAALACVGG FVLGGAFGVF TAGIDTNVGF DPKDPYRTPT AREVLKDMGQ
     RGMSYAKNFA IVGAMFSCTE CLVESYRGKS DWKNSVISGC ITGGAIGFRA GLKAGVIGCG
     GFAAFSAAID YYLR