TIM22_CANAL
ID TIM22_CANAL Reviewed; 184 AA.
AC A0A1D8PI78;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 18-JAN-2017, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN Name=TIM22 {ECO:0000312|CGD:CAL0000200177};
GN OrderedLocusNames=CAALFM_C208310WA {ECO:0000312|EMBL:AOW27821.1};
GN ORFNames=orf19.1352 {ECO:0000312|CGD:CAL0000200177};
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [4]
RP DISULFIDE BOND, AND MUTAGENESIS OF CYS-118 AND CYS-156.
RX PubMed=27265872; DOI=10.1038/srep27484;
RA Wrobel L., Sokol A.M., Chojnacka M., Chacinska A.;
RT "The presence of disulfide bonds reveals an evolutionarily conserved
RT mechanism involved in mitochondrial protein translocase assembly.";
RL Sci. Rep. 6:27484-27484(2016).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. In the TIM22 complex, it
CC constitutes the voltage-activated and signal-gated channel. Forms a
CC twin-pore translocase that uses the membrane potential as external
CC driving force in 2 voltage-dependent steps (By similarity).
CC {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; CP017624; AOW27821.1; -; Genomic_DNA.
DR RefSeq; XP_710236.2; XM_705144.2.
DR AlphaFoldDB; A0A1D8PI78; -.
DR SMR; A0A1D8PI78; -.
DR STRING; 237561.A0A1D8PI78; -.
DR GeneID; 3648168; -.
DR KEGG; cal:CAALFM_C208310WA; -.
DR CGD; CAL0000200177; TIM22.
DR VEuPathDB; FungiDB:C2_08310W_A; -.
DR eggNOG; KOG3225; Eukaryota.
DR OMA; THSYAKN; -.
DR OrthoDB; 1544601at2759; -.
DR Proteomes; UP000000559; Chromosome 2.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:CGD.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 1: Evidence at protein level;
KW Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..184
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM22"
FT /id="PRO_0000442001"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 40..118
FT /evidence="ECO:0000305|PubMed:27265872"
FT DISULFID 137..156
FT /evidence="ECO:0000305|PubMed:27265872"
FT MUTAGEN 118
FT /note="C->S: Abolishes disulfide bonds; when associated
FT with S-156."
FT /evidence="ECO:0000269|PubMed:27265872"
FT MUTAGEN 156
FT /note="C->S: Abolishes disulfide bonds; when associated
FT with S-118."
FT /evidence="ECO:0000269|PubMed:27265872"
SQ SEQUENCE 184 AA; 19636 MW; BEDF30208452D149 CRC64;
MSLWGVYTGP QPPKKPLQEM TQEEQAEEGA RQMIGFMNSC PGKTVMAGVS GFALGGFFGL
FMASMAYDTP IGTDAVKHIS ELPFKQQMKL QFTDMAKRSY SSAKNFGYIG MVYSGVECTI
ESLRAKHDIY NGVSAGCITG AGLAIRAGPQ AALVGCAGFA AFSLAIDMYL NSDAAPPPKN
DYDI
