ID   TIM22_CANGA             Reviewed;         193 AA.
AC   Q6FT37;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN   Name=TIM22; OrderedLocusNames=CAGL0G05654g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; CR380953; CAG59534.1; -; Genomic_DNA.
DR   RefSeq; XP_446607.1; XM_446607.1.
DR   AlphaFoldDB; Q6FT37; -.
DR   SMR; Q6FT37; -.
DR   STRING; 5478.XP_446607.1; -.
DR   EnsemblFungi; CAG59534; CAG59534; CAGL0G05654g.
DR   GeneID; 2888408; -.
DR   KEGG; cgr:CAGL0G05654g; -.
DR   CGD; CAL0130616; CAGL0G05654g.
DR   VEuPathDB; FungiDB:CAGL0G05654g; -.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_091077_1_0_1; -.
DR   InParanoid; Q6FT37; -.
DR   Proteomes; UP000002428; Chromosome G.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:EnsemblFungi.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IEA:EnsemblFungi.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IEA:EnsemblFungi.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:EnsemblFungi.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..193
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM22"
FT                   /id="PRO_0000228088"
FT   TRANSMEM        47..67
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..154
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        42..127
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT   DISULFID        146..165
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ   SEQUENCE   193 AA;  20242 MW;  71FA91A98E6401BD CRC64;
     MVYRGFGLEY LSPPEKKAFG ELSPDEQGER GAEMVVGFMS SCPGKSVISG ATGFALGGVL
     GLFMASMAYD TPLHTPVPGG MSGAVQQMAD LPLRQQVKLQ FADMGKRAYS SAKNFGYIGM
     IYAGVECAVE SLRAKNDIYN GITAGCITGG GLAYKSGPQA ALVGCAGFAA FSAAIDMYMK
     SEDGRPPEND FKQ