TIM22_CRYNJ
ID TIM22_CRYNJ Reviewed; 187 AA.
AC P0CR88; Q55VM2; Q5KKL8;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 40.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN Name=TIM22; OrderedLocusNames=CNC02580;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. In the TIM22 complex, it
CC constitutes the voltage-activated and signal-gated channel. Forms a
CC twin-pore translocase that uses the membrane potential as external
CC driving force in 2 voltage-dependent steps (By similarity).
CC {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; AE017343; AAW42244.1; -; Genomic_DNA.
DR RefSeq; XP_569551.1; XM_569551.1.
DR AlphaFoldDB; P0CR88; -.
DR SMR; P0CR88; -.
DR STRING; 5207.AAW42244; -.
DR PaxDb; P0CR88; -.
DR EnsemblFungi; AAW42244; AAW42244; CNC02580.
DR GeneID; 3256470; -.
DR KEGG; cne:CNC02580; -.
DR VEuPathDB; FungiDB:CNC02580; -.
DR eggNOG; KOG3225; Eukaryota.
DR HOGENOM; CLU_091077_1_0_1; -.
DR InParanoid; P0CR88; -.
DR OMA; QGMESCA; -.
DR OrthoDB; 1544601at2759; -.
DR Proteomes; UP000002149; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..187
FT /note="Mitochondrial import inner membrane translocase
FT subunit TIM22"
FT /id="PRO_0000228089"
FT TRANSMEM 58..78
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DISULFID 52..127
FT /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ SEQUENCE 187 AA; 20059 MW; 3B232DF3CEF6B8B5 CRC64;
MSIPLPSAMP LLPPIYLPGQ EPLPAGTSDW ERQEMQTALK YQRYMGMVME SCPLKVTIAG
VGGLAIGGFF SLMSATFAYE DPLSRASNKL TTTRAQTMFV FKEMGRNMWS SGRGFAKVGM
VYSGVECCIE GYRAKNDIYN GVSAGFLTGA ILARNAGPTA MLGGGVAFAA FSGAIDWWLR
SAPADEI