ID   TIM22_DEBHA             Reviewed;         182 AA.
AC   Q6BT35;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit TIM22;
GN   Name=TIM22; OrderedLocusNames=DEHA2D03872g;
OS   Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990
OS   / NBRC 0083 / IGC 2968) (Yeast) (Torulaspora hansenii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Debaryomyces.
OX   NCBI_TaxID=284592;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC       TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; CR382136; CAG86774.1; -; Genomic_DNA.
DR   RefSeq; XP_458635.1; XM_458635.1.
DR   AlphaFoldDB; Q6BT35; -.
DR   SMR; Q6BT35; -.
DR   STRING; 4959.XP_458635.1; -.
DR   EnsemblFungi; CAG86774; CAG86774; DEHA2D03872g.
DR   GeneID; 2901154; -.
DR   KEGG; dha:DEHA2D03872g; -.
DR   VEuPathDB; FungiDB:DEHA2D03872g; -.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_091077_1_0_1; -.
DR   InParanoid; Q6BT35; -.
DR   OMA; THSYAKN; -.
DR   OrthoDB; 1544601at2759; -.
DR   Proteomes; UP000000599; Chromosome D.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IEA:InterPro.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IEA:InterPro.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..182
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit TIM22"
FT                   /id="PRO_0000228090"
FT   TRANSMEM        43..63
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..143
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        38..116
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT   DISULFID        135..154
FT                   /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
SQ   SEQUENCE   182 AA;  19502 MW;  D04E42427C1BD330 CRC64;
     MAFGVYKVPE EQKTYAQMTP QEQAEEGAKK MVELMQSCPG KTVMAGVSGF FLGGFFGLFM
     ASMSYDVPIG TNAVSNIRDL PFKQQMKLQF SDMGKRTYSS AKNFGYIGMV YSGVECAIES
     LRAKHDIYNG VSAGCITGGG LAIRAGPQAA LVGCAGFAAF STAIDLYLRS DSASPPKNDY
     DE