ID   TIM22_DICDI             Reviewed;         179 AA.
AC   Q54QM0;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit tim22;
GN   Name=timm22; Synonyms=tim22; ORFNames=DDB_G0283865;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC       timm22, associated with the 70 kDa heterohexamer composed of timm9 and
CC       timm10. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000250};
CC       Multi-pass membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; AAFI02000057; EAL65547.1; -; Genomic_DNA.
DR   RefSeq; XP_638851.1; XM_633759.1.
DR   AlphaFoldDB; Q54QM0; -.
DR   SMR; Q54QM0; -.
DR   STRING; 44689.DDB0218527; -.
DR   PaxDb; Q54QM0; -.
DR   EnsemblProtists; EAL65547; EAL65547; DDB_G0283865.
DR   GeneID; 8624249; -.
DR   KEGG; ddi:DDB_G0283865; -.
DR   dictyBase; DDB_G0283865; timm22.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_1506115_0_0_1; -.
DR   InParanoid; Q54QM0; -.
DR   OMA; THSYAKN; -.
DR   PRO; PR:Q54QM0; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   3: Inferred from homology;
KW   Membrane; Mitochondrion; Mitochondrion inner membrane; Protein transport;
KW   Reference proteome; Translocation; Transmembrane; Transmembrane helix;
KW   Transport.
FT   CHAIN           1..179
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit tim22"
FT                   /id="PRO_0000331557"
FT   TRANSMEM        59..79
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   179 AA;  19475 MW;  0AC27365939AB332 CRC64;
     MANISDEELK KILSDNAHKF MFAENGVRDF LPNIKNIAPY NEMQYNLMDN CIVHGVRGMV
     MGGAFGFLFG ALFTPNSGFT PEPTTPTPLY RQVIDGFKEQ GRSGLRSAKS LSIITLVYTG
     TECAIEKARG RTDKLNPIYA GCTTGAVFAG RAGPMAAVGG CVGFAVFGMI MDHFMVFFF