TIM22_NEUCR
ID TIM22_NEUCR Reviewed; 194 AA.
AC Q9C1E8; Q1K7H2; Q9HEE8;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 2.
DT 25-MAY-2022, entry version 85.
DE RecName: Full=Mitochondrial import inner membrane translocase subunit tim22;
GN Name=tim22; ORFNames=99H12.010, NCU03798;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=14668492; DOI=10.1091/mbc.e03-05-0272;
RA Vasiljev A., Ahting U., Nargang F.E., Go N.E., Habib S.J., Kozany C.,
RA Panneels V., Sinning I., Prokisch H., Neupert W., Nussberger S.,
RA Rapaport D.;
RT "Reconstituted TOM core complex and Tim9/Tim10 complex of mitochondria are
RT sufficient for translocation of the ADP/ATP carrier across membranes.";
RL Mol. Biol. Cell 15:1445-1458(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC mediates the import and insertion of multi-pass transmembrane proteins
CC into the mitochondrial inner membrane. In the TIM22 complex, it
CC constitutes the voltage-activated and signal-gated channel. Forms a
CC twin-pore translocase that uses the membrane potential as external
CC driving force in 2 voltage-dependent steps (By similarity).
CC {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBUNIT: Component of the TIM22 complex, whose core is composed of
CC TIM22 and TIM54, associated with the 70 kDa heterohexamer composed of
CC TIM9 and TIM10 (or TIM8 and TIM13). {ECO:0000250|UniProtKB:Q12328}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000250|UniProtKB:Q12328}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR EMBL; AF343074; AAK26643.1; -; Genomic_DNA.
DR EMBL; AL451018; CAC18237.2; -; Genomic_DNA.
DR EMBL; CM002240; EAA31951.3; -; Genomic_DNA.
DR RefSeq; XP_961187.3; XM_956094.3.
DR AlphaFoldDB; Q9C1E8; -.
DR SMR; Q9C1E8; -.
DR STRING; 5141.EFNCRP00000003464; -.
DR EnsemblFungi; EAA31951; EAA31951; NCU03798.
DR GeneID; 3877347; -.
DR KEGG; ncr:NCU03798; -.
DR VEuPathDB; FungiDB:NCU03798; -.
DR HOGENOM; CLU_091077_1_0_1; -.
DR InParanoid; Q9C1E8; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; IBA:GO_Central.
DR GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; IBA:GO_Central.
DR InterPro; IPR039175; TIM22.
DR PANTHER; PTHR14110; PTHR14110; 1.
PE 3: Inferred from homology;
KW Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Protein transport; Reference proteome; Translocation; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..194
FT /note="Mitochondrial import inner membrane translocase
FT subunit tim22"
FT /id="PRO_0000228092"
FT TRANSMEM 43..63
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 145..161
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 38..135
FT /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT DISULFID 154..173
FT /evidence="ECO:0000250|UniProtKB:A0A1D8PI78"
FT CONFLICT 72
FT /note="P -> S (in Ref. 1; AAK26643)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 194 AA; 19511 MW; 84941946CC33D031 CRC64;
MNFPGMPGGA APSGGAAPGG YDPNDPNIKM MQKAMESCFA KTVMSGGAGF ALGGVFGMFM
ASMAYDTPYH SPTTPGTGPG ANPAAAGIPG YKPVDLSSMP LKEQLKHGFK DMGQRSYSTA
KNFAKVGALF SGIECGIEGL RAKNDLGNGV AAGCLTGAIL AKNGGPQAAA VGCAGFAAFS
AAIDAWMRMP SEED
