ID   TIM22_XENLA             Reviewed;         184 AA.
AC   Q5U4U5;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 55.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim22;
GN   Name=timm22; Synonyms=tim22;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Core component of the TIM22 complex.
CC       {ECO:0000250|UniProtKB:Q9Y584}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y584}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; BC084949; AAH84949.1; -; mRNA.
DR   RefSeq; NP_001088555.2; NM_001095086.1.
DR   AlphaFoldDB; Q5U4U5; -.
DR   SMR; Q5U4U5; -.
DR   DNASU; 495431; -.
DR   GeneID; 495431; -.
DR   CTD; 495431; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:UniProtKB.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..184
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim22"
FT                   /id="PRO_0000228082"
FT   TRANSMEM        64..84
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..133
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        59..131
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
FT   DISULFID        150..169
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
SQ   SEQUENCE   184 AA;  19389 MW;  052CC662F2A57D7E CRC64;
     MGSNVTPPGD GTLQYSLIMQ HLVGDKRRPV ELIPGGLGGI PTPIKPEEQK MMERVMESCG
     FKAALACVGG FVLGGAFGVF TAGIDTNVGF DPKDPLRTPT AKEVLRDMGQ RGMSYAKNFA
     IVGAMFSCTE CLVESYRGKS DWKNSVMSGC ITGGAIGFRA GLKAGVLGCG GFAAFSAVID
     YYLR