ID   TIM22_XENTR             Reviewed;         186 AA.
AC   Q5M7K0;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Mitochondrial import inner membrane translocase subunit Tim22;
GN   Name=timm22; Synonyms=tim22;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential core component of the TIM22 complex, a complex that
CC       mediates the import and insertion of multi-pass transmembrane proteins
CC       into the mitochondrial inner membrane. In the TIM22 complex, it
CC       constitutes the voltage-activated and signal-gated channel. Forms a
CC       twin-pore translocase that uses the membrane potential as external
CC       driving force in 2 voltage-dependent steps (By similarity).
CC       {ECO:0000250|UniProtKB:Q12328}.
CC   -!- SUBUNIT: Core component of the TIM22 complex.
CC       {ECO:0000250|UniProtKB:Q9Y584}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC       {ECO:0000250|UniProtKB:Q9Y584}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the Tim17/Tim22/Tim23 family. {ECO:0000305}.
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DR   EMBL; BC088600; AAH88600.1; -; mRNA.
DR   RefSeq; NP_001011397.1; NM_001011397.1.
DR   AlphaFoldDB; Q5M7K0; -.
DR   SMR; Q5M7K0; -.
DR   STRING; 8364.ENSXETP00000037599; -.
DR   PaxDb; Q5M7K0; -.
DR   Ensembl; ENSXETT00000041721; ENSXETP00000041721; ENSXETG00000019259.
DR   GeneID; 496870; -.
DR   KEGG; xtr:496870; -.
DR   CTD; 29928; -.
DR   Xenbase; XB-GENE-5754351; timm22.
DR   eggNOG; KOG3225; Eukaryota.
DR   HOGENOM; CLU_091077_0_0_1; -.
DR   InParanoid; Q5M7K0; -.
DR   OrthoDB; 1544601at2759; -.
DR   PhylomeDB; Q5M7K0; -.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   Bgee; ENSXETG00000019259; Expressed in egg cell and 12 other tissues.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0042721; C:TIM22 mitochondrial import inner membrane insertion complex; ISS:UniProtKB.
DR   GO; GO:0030943; F:mitochondrion targeting sequence binding; IBA:GO_Central.
DR   GO; GO:0008320; F:protein transmembrane transporter activity; IBA:GO_Central.
DR   GO; GO:0045039; P:protein insertion into mitochondrial inner membrane; ISS:UniProtKB.
DR   InterPro; IPR039175; TIM22.
DR   PANTHER; PTHR14110; PTHR14110; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW   Protein transport; Reference proteome; Translocation; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..186
FT                   /note="Mitochondrial import inner membrane translocase
FT                   subunit Tim22"
FT                   /id="PRO_0000228083"
FT   TRANSMEM        66..86
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DISULFID        61..133
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
FT   DISULFID        152..171
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y584"
SQ   SEQUENCE   186 AA;  19472 MW;  49823AA32FFDFCCA CRC64;
     MGGSVSPPGT GEGTLQYSLI MEHLVGDKRR PKEVIPGGLG GIPTPIKSEE QKMMERVMES
     CGFKAALACV GGFVLGGAFG VFTAGIDTNV GFDPKDPYRT PTAKEVLKDM GQRGMSYAKN
     FAIVGAMFSC TECLVESYRG KSDWKNSVIS GCITGGAIGF RAGLKAGALG CGGFAAFSAV
     IDYYLR